Single guide RNA library design and construction

Cold Spring Harbor Protocols

Volumn 2016, Issue 3, 2016, Pages 283-288

  Wang, Tim ^a,b,c   Lander, Eric S ^a,c,d   Sabatini, David M ^a,b,c  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^c BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; GENE AMPLIFICATION; GENE SEQUENCE; GENE TARGETING; GENETIC SCREENING; MOLECULAR CLONING; MOLECULAR LIBRARY; NONHUMAN; POLYMERASE CHAIN REACTION; SINGLE GUIDE RNA LIBRARY; ANIMAL; GENE INACTIVATION; GENE LIBRARY; GENETICS; HUMAN; MOUSE; PROCEDURES;

RNA;

ANIMALS; CRISPR-CAS SYSTEMS; GENE KNOCKOUT TECHNIQUES; GENE LIBRARY; GENETIC TESTING; HUMANS; MICE; RNA;

EID: 84959495011     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot090803     Document Type: Article

References (2)

1. Heigwer F, Kerr G, Boutros M. 2014. E-CRISP: Fast CRISPR target site identification. Nat Methods 11: 122-123.
2. Xie S, Shen B, Zhang C, Huang X, Zhang Y. 2014. sgRNAcas9: A software package for designing CRISPR sgRNA and evaluating potential offtarget cleavage sites. PLoS One 9: e100448.