Generation of mouse and human dendritic cells in vitro

Journal of Immunological Methods

Volumn 432, Issue , 2016, Pages 24-29

  Guo, Xueheng ^a   Zhou, Yifan ^a   Wu, Tao ^a   Zhu, Xinyi ^a   Lai, Wenlong ^a   Wu, Li ^a  

^a Tsinghua University   (China)

Author keywords

Dendritic cells; Flt3L; GM CSF; In vitro culture

Indexed keywords

CD34 ANTIGEN; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; BIOLOGICAL MARKER;

ANTIGEN PRESENTATION; ARTICLE; BONE MARROW CELL; CELL DIFFERENTIATION; CELL MIGRATION; DENDRITIC CELL; EMBRYONIC STEM CELL; GENE EXPRESSION; GRANULOCYTE; HEMATOPOIETIC STEM CELL; HUMAN; IN VITRO STUDY; LANGERHANS CELL; MACROPHAGE; MONOCYTE; MOUSE; NATURAL KILLER CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; STEM CELL; UMBILICAL CORD BLOOD; ANIMAL; CELL CULTURE; CELL CULTURE TECHNIQUE; CELL LINEAGE; CELL PROLIFERATION; IMMUNOLOGY; INFLAMMATION; METABOLISM; PHENOTYPE; SPECIES DIFFERENCE;

ANIMALS; BIOMARKERS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; CELL PROLIFERATION; CELLS, CULTURED; DENDRITIC CELLS; HUMANS; INFLAMMATION; MICE; PHENOTYPE; SPECIES SPECIFICITY;

EID: 84958087387     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2016.02.011     Document Type: Article

References (75)

1. Aarntzen E.H., De Vries I.J., Lesterhuis W.J., Schuurhuis D., Jacobs J.F., Bol K., Schreibelt G., Mus R., De Wilt J.H., Haanen J.B., Schadendorf D., Croockewit A., Blokx W.A., Van Rossum M.M., Kwok W.W., Adema G.J., Punt C.J., Figdor C.G. Targeting CD4(+) T-helper cells improves the induction of antitumor responses in dendritic cell-based vaccination. Cancer Res. 2013, 73:19-29.
2. Allan R.S., Smith C.M., Belz G.T., van Lint A.L., Wakim L.M., Heath W.R., Carbone F.R. Epidermal viral immunity induced by CD8alpha + dendritic cells but not by Langerhans cells. Science 2003, 301:1925-1928.
3. Bachem A., Guttler S., Hartung E., Ebstein F., Schaefer M., Tannert A., Salama A., Movassaghi K., Opitz C., Mages H.W., Henn V., Kloetzel P.M., Gurka S., Kroczek R.A. Superior antigen cross-presentation and XCR1 expression define human CD11c + CD141+ cells as homologues of mouse CD8+ dendritic cells. J. Exp. Med. 2010, 207:1273-1281.
4. Balan S., Kale V.P., Limaye L.S. A simple two-step culture system for the large-scale generation of mature and functional dendritic cells from umbilical cord blood CD34+ cells. Transfusion 2009, 49:2109-2121.
5. Balan S., Kale V.P., Limaye L.S. A large number of mature and functional dendritic cells can be efficiently generated from umbilical cord blood-derived mononuclear cells by a simple two-step culture method. Transfusion 2010, 50:2413-2423.
6. Balan S., Ollion V., Colletti N., Chelbi R., Montanana-Sanchis F., Liu H., Vu Manh T.P., Sanchez C., Savoret J., Perrot I., Doffin A.C., Fossum E., Bechlian D., Chabannon C., Bogen B., Asselin-Paturel C., Shaw M., Soos T., Caux C., Valladeau-Guilemond J., Dalod M. Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells. J. Immunol. 2014, 193:1622-1635.
7. Becker M.R., Choi Y.S., Millar S.E., Udey M.C. Wnt signaling influences the development of murine epidermal Langerhans cells. J. Invest. Dermatol. 2011, 131:1861-1868.
8. Belz G.T., Nutt S.L. Transcriptional programming of the dendritic cell network. Nat. Rev. Immunol. 2012, 12:101-113.
9. Borkowski T.A., Letterio J.J., Farr A.G., Udey M.C. A role for endogenous transforming growth factor beta 1 in Langerhans cell biology: the skin of transforming growth factor beta 1 null mice is devoid of epidermal Langerhans cells. J. Exp. Med. 1996, 184:2417-2422.
10. Borkowski T.A., Letterio J.J., Mackall C.L., Saitoh A., Wang X.J., Roop D.R., Gress R.E., Udey M.C. A role for TGFbeta1 in langerhans cell biology. Further characterization of the epidermal Langerhans cell defect in TGFbeta1 null mice. J. Clin. Invest. 1997, 100:575-581.
11. Breton G., Lee J., Zhou Y.J., Schreiber J.J., Keler T., Puhr S., Anandasabapathy N., Schlesinger S., Caskey M., Liu K., Nussenzweig M.C. Circulating precursors of human CD1c + and CD141+ dendritic cells. J. Exp. Med. 2015, 212:401-413.
12. Caux C., Vanbervliet B., Massacrier C., Dezutter-Dambuyant C., de Saint-Vis B., Jacquet C., Yoneda K., Imamura S., Schmitt D., Banchereau J. CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF + TNF alpha. J. Exp. Med. 1996, 184:695-706.
13. Cebrian I., Visentin G., Blanchard N., Jouve M., Bobard A., Moita C., Enninga J., Moita L.F., Amigorena S., Savina A. Sec22b regulates phagosomal maturation and antigen crosspresentation by dendritic cells. Cell 2011, 147:1355-1368.
14. Chaperot L., Perrot I., Jacob M.C., Blanchard D., Salaun V., Deneys V., Lebecque S., Briere F., Bensa J.C., Plumas J. Leukemic plasmacytoid dendritic cells share phenotypic and functional features with their normal counterparts. Eur. J. Immunol. 2004, 34:418-426.
15. Chopin M., Allan R.S., Belz G.T. Transcriptional regulation of dendritic cell diversity. Front. Immunol. 2012, 3:26.
16. Chopin M., Seillet C., Chevrier S., Wu L., Wang H., Morse H.C., Belz G.T., Nutt S.L. Langerhans cells are generated by two distinct PU.1-dependent transcriptional networks. J. Exp. Med. 2013, 210:2967-2980.
17. Curti A., Fogli M., Ratta M., Tura S., Lemoli R.M. Stem cell factor and FLT3-ligand are strictly required to sustain the long-term expansion of primitive CD34 + DR-dendritic cell precursors. J. Immunol. 2001, 166:848-854.
18. Demoulin S., Roncarati P., Delvenne P., Hubert P. Production of large numbers of plasmacytoid dendritic cells with functional activities from CD34(+) hematopoietic progenitor cells: use of interleukin-3. Exp. Hematol. 2012, 40:268-278.
19. Dubsky P., Saito H., Leogier M., Dantin C., Connolly J.E., Banchereau J., Palucka A.K. IL-15-induced human DC efficiently prime melanoma-specific naive CD8+ T cells to differentiate into CTL. Eur. J. Immunol. 2007, 37:1678-1690.
20. Durand M., Segura E. The known unknowns of the human dendritic cell network. Front. Immunol. 2015, 6:129.
21. Fowler D.W., Copier J., Wilson N., Dalgleish A.G., Bodman-Smith M.D. Mycobacteria activate gammadelta T-cell anti-tumour responses via cytokines from type 1 myeloid dendritic cells: a mechanism of action for cancer immunotherapy. Cancer Immunol. Immunother. 2012, 61:535-547.
22. Fuertes Marraco S.A., Grosjean F., Duval A., Rosa M., Lavanchy C., Ashok D., Haller S., Otten L.A., Steiner Q.G., Descombes P., Luber C.A., Meissner F., Mann M., Szeles L., Reith W., Acha-Orbea H. Novel murine dendritic cell lines: a powerful auxiliary tool for dendritic cell research. Front. Immunol. 2012, 3:331.
23. Garcia F., Climent N., Guardo A.C., Gil C., Leon A., Autran B., Lifson J.D., Martinez-Picado J., Dalmau J., Clotet B., Gatell J.M., Plana M., Gallart T., Group D.M.O.S. A dendritic cell-based vaccine elicits T cell responses associated with control of HIV-1 replication. Sci Transl Med 2013, 5.
24. Hanke N.T., LaCasse C.J., Larmonier C.B., Alizadeh D., Trad M., Janikashvili N., Bonnotte B., Katsanis E., Larmonier N. PIAS1 and STAT-3 impair the tumoricidal potential of IFN-gamma-stimulated mouse dendritic cells generated with IL-15. Eur. J. Immunol. 2014, 44:2489-2499.
25. Helft J., Bottcher J., Chakravarty P., Zelenay S., Huotari J., Schraml B.U., Goubau D., Reis E.S.C. GM-CSF mouse bone marrow cultures comprise a heterogeneous population of CD11c(+)MHCII(+) macrophages and dendritic cells. Immunity 2015, 42:1197-1211.
26. Hiasa M., Abe M., Nakano A., Oda A., Amou H., Kido S., Takeuchi K., Kagawa K., Yata K., Hashimoto T., Ozaki S., Asaoka K., Tanaka E., Moriyama K., Matsumoto T. GM-CSF and IL-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through M-CSF receptor shedding by up-regulation of TNF-alpha converting enzyme (TACE). Blood 2009, 114:4517-4526.
27. Hubert P., Bousarghin L., Greimers R., Franzen-Detrooz E., Boniver J., Delvenne P. Production of large numbers of Langerhans' cells with intraepithelial migration ability in vitro. Exp. Dermatol. 2005, 14:469-477.
28. Hwang E.C., Lim M.S., Im C.M., Kwon D.D., Lee H.J., Nguyen-Pham T.N., Lee Y.K., Lee J.J. Generation of potent cytotoxic T lymphocytes against castration-resistant prostate cancer cells by dendritic cells loaded with dying allogeneic prostate cancer cells. Scand. J. Immunol. 2013, 77:117-124.
29. Inaba K., Inaba M., Romani N., Aya H., Deguchi M., Ikehara S., Muramatsu S., Steinman R.M. Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 1992, 176:1693-1702.
30. Ji J., Vijayaragavan K., Bosse M., Menendez P., Weisel K., Bhatia M. OP9 stroma augments survival of hematopoietic precursors and progenitors during hematopoietic differentiation from human embryonic stem cells. Stem Cells 2008, 26:2485-2495.
31. Jones H.E., Klein N., Dixon G.L. Human dendritic cell culture and bacterial infection. Methods Mol. Biol. 2012, 799:217-235.
32. Jongbloed S.L., Kassianos A.J., McDonald K.J., Clark G.J., Ju X., Angel C.E., Chen C.J., Dunbar P.R., Wadley R.B., Jeet V., Vulink A.J., Hart D.N., Radford K.J. Human CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens. J. Exp. Med. 2010, 207:1247-1260.
33. Jonuleit H., Kuhn U., Muller G., Steinbrink K., Paragnik L., Schmitt E., Knop J., Enk A.H. Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 1997, 27:3135-3142.
34. Klechevsky E., Banchereau J. Human dendritic cells subsets as targets and vectors for therapy. Ann. N. Y. Acad. Sci. 2013, 1284:24-30.
35. Klechevsky E., Morita R., Liu M., Cao Y., Coquery S., Thompson-Snipes L., Briere F., Chaussabel D., Zurawski G., Palucka A.K., Reiter Y., Banchereau J., Ueno H. Functional specializations of human epidermal Langerhans cells and CD14+ dermal dendritic cells. Immunity 2008, 29:497-510.
36. Labeur M.S., Roters B., Pers B., Mehling A., Luger T.A., Schwarz T., Grabbe S. Generation of tumor immunity by bone marrow-derived dendritic cells correlates with dendritic cell maturation stage. J. Immunol. 1999, 162:168-175.
37. Lee J., Breton G., Oliveira T.Y., Zhou Y.J., Aljoufi A., Puhr S., Cameron M.J., Sekaly R.P., Nussenzweig M.C., Liu K. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J. Exp. Med. 2015, 212:385-399.
38. Leon B., Martinez del Hoyo G., Parrillas V., Vargas H.H., Sanchez-Mateos P., Longo N., Lopez-Bravo M., Ardavin C. Dendritic cell differentiation potential of mouse monocytes: monocytes represent immediate precursors of CD8- and CD8+ splenic dendritic cells. Blood 2004, 103:2668-2676.
39. Lutz M.B., Kukutsch N., Ogilvie A.L., Rossner S., Koch F., Romani N., Schuler G. An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J. Immunol. Methods 1999, 223:77-92.
40. Mayer C.T., Ghorbani P., Nandan A., Dudek M., Arnold-Schrauf C., Hesse C., Berod L., Stuve P., Puttur F., Merad M., Sparwasser T. Selective and efficient generation of functional Batf3-dependent CD103+ dendritic cells from mouse bone marrow. Blood 2014, 124:3081-3091.
41. Merad M., Manz M.G. Dendritic cell homeostasis. Blood 2009, 113:3418-3427.
42. Merad M., Sathe P., Helft J., Miller J., Mortha A. The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting. Annu. Rev. Immunol. 2013, 31:563-604.
43. Mohib K., Wang L. Differentiation and characterization of dendritic cells from human embryonic stem cells. Curr. Protoc. Immunol. 2012, (Chapter 22, Unit 22F 11).
44. Nagao K., Ginhoux F., Leitner W.W., Motegi S., Bennett C.L., Clausen B.E., Merad M., Udey M.C. Murine epidermal Langerhans cells and langerin-expressing dermal dendritic cells are unrelated and exhibit distinct functions. Proc. Natl. Acad. Sci. U. S. A. 2009, 106:3312-3317.
45. Naik S.H., Proietto A.I., Wilson N.S., Dakic A., Schnorrer P., Fuchsberger M., Lahoud M.H., O'Keeffe M., Shao Q.X., Chen W.F., Villadangos J.A., Shortman K., Wu L. Cutting edge: generation of splenic CD8+ and CD8- dendritic cell equivalents in fms-like tyrosine kinase 3 ligand bone marrow cultures. J. Immunol. 2005, 174:6592-6597.
46. Naik S.H., Sathe P., Park H.Y., Metcalf D., Proietto A.I., Dakic A., Carotta S., O'Keeffe M., Bahlo M., Papenfuss A., Kwak J.Y., Wu L., Shortman K. Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo. Nat. Immunol. 2007, 8:1217-1226.
47. Nair S., Archer G.E., Tedder T.F. Isolation and generation of human dendritic cells. Curr. Protoc. Immunol. 2012, (Chapter 7, Unit7 32).
48. Nishimoto K.P., Tseng S.Y., Lebkowski J.S., Reddy A. Modification of human embryonic stem cell-derived dendritic cells with mRNA for efficient antigen presentation and enhanced potency. Regen. Med. 2011, 6:303-318.
49. Nuyts A.H., Ponsaerts P., Van Tendeloo V.F., Lee W.P., Stein B., Nagels G., D'Hooghe M.B., Willekens B., Cras P., Wouters K., Goossens H., Berneman Z.N., Cools N. Except for C-C chemokine receptor 7 expression, monocyte-derived dendritic cells from patients with multiple sclerosis are functionally comparable to those of healthy controls. Cytotherapy 2014, 16:1024-1030.
50. Palucka K., Banchereau J. Cancer immunotherapy via dendritic cells. Nat. Rev. Cancer 2012, 12:265-277.
51. Poulin L.F., Salio M., Griessinger E., Anjos-Afonso F., Craciun L., Chen J.L., Keller A.M., Joffre O., Zelenay S., Nye E., Le Moine A., Faure F., Donckier V., Sancho D., Cerundolo V., Bonnet D., Reis e Sousa C. Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells. J. Exp. Med. 2010, 207:1261-1271.
52. Proietto A.I., Mittag D., Roberts A.W., Sprigg N., Wu L. The equivalents of human blood and spleen dendritic cell subtypes can be generated in vitro from human CD34(+) stem cells in the presence of fms-like tyrosine kinase 3 ligand and thrombopoietin. Cell. Mol. Immunol. 2012, 9:446-454.
53. Pulendran B., Dillon S., Joseph C., Curiel T., Banchereau J., Mohamadzadeh M. Dendritic cells generated in the presence of GM-CSF plus IL-15 prime potent CD8+ Tc1 responses in vivo. Eur. J. Immunol. 2004, 34:66-73.
54. Sallusto F., Lanzavecchia A. Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. J. Exp. Med. 1994, 179:1109-1118.
55. Sathe P., Pooley J., Vremec D., Mintern J., Jin J.O., Wu L., Kwak J.Y., Villadangos J.A., Shortman K. The acquisition of antigen cross-presentation function by newly formed dendritic cells. J. Immunol. 2011, 186:5184-5192.
56. Savina A., Jancic C., Hugues S., Guermonprez P., Vargas P., Moura I.C., Lennon-Dumenil A.M., Seabra M.C., Raposo G., Amigorena S. NOX2 controls phagosomal pH to regulate antigen processing during crosspresentation by dendritic cells. Cell 2006, 126:205-218.
57. Savina A., Peres A., Cebrian I., Carmo N., Moita C., Hacohen N., Moita L.F., Amigorena S. The small GTPase Rac2 controls phagosomal alkalinization and antigen crosspresentation selectively in CD8(+) dendritic cells. Immunity 2009, 30:544-555.
58. Savina A., Vargas P., Guermonprez P., Lennon A.M., Amigorena S. Measuring pH, ROS production, maturation, and degradation in dendritic cell phagosomes using cytofluorometry-based assays. Methods Mol. Biol. 2010, 595:383-402.
59. Schlitzer A., McGovern N., Ginhoux F. Dendritic cells and monocyte-derived cells: ttwo complementary and integrated functional systems. Semin. Cell Dev. Biol. 2015.
60. Schmid M.A., Kingston D., Boddupalli S., Manz M.G. Instructive cytokine signals in dendritic cell lineage commitment. Immunol. Rev. 2010, 234:32-44.
61. Schotte R., Schmidlin H., Nagasawa M., Dontje W., Karrich J.J., Uittenbogaart C., Spits H., Blom B. Isolation and in vitro generation of gene-manipulated human plasmacytoid and conventional dendritic cells. Methods Mol. Biol. 2010, 595:67-85.
62. Schreurs M.W., Eggert A.A., de Boer A.J., Figdor C.G., Adema G.J. Generation and functional characterization of mouse monocyte-derived dendritic cells. Eur. J. Immunol. 1999, 29:2835-2841.
63. Silk K.M., Silk J.D., Ichiryu N., Davies T.J., Nolan K.F., Leishman A.J., Carpenter L., Watt S.M., Cerundolo V., Fairchild P.J. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells. Gene Ther. 2012, 19:1035-1040.
64. Steiner Q.G., Otten L.A., Hicks M.J., Kaya G., Grosjean F., Saeuberli E., Lavanchy C., Beermann F., McClain K.L., Acha-Orbea H. In vivo transformation of mouse conventional CD8alpha+ dendritic cells leads to progressive multisystem histiocytosis. Blood 2008, 111:2073-2082.
65. Steinman R.M. Decisions about dendritic cells: past, present, and future. Annu. Rev. Immunol. 2012, 30:1-22.
66. Teijeira A., Russo E., Halin C. Taking the lymphatic route: dendritic cell migration to draining lymph nodes. Semin. Immunopathol. 2014, 36:261-274.
67. Tseng S.Y., Nishimoto K.P., Silk K.M., Majumdar A.S., Dawes G.N., Waldmann H., Fairchild P.J., Lebkowski J.S., Reddy A. Generation of immunogenic dendritic cells from human embryonic stem cells without serum and feeder cells. Regen. Med. 2009, 4:513-526.
68. Tuyaerts S., Aerts J.L., Corthals J., Neyns B., Heirman C., Breckpot K., Thielemans K., Bonehill A. Current approaches in dendritic cell generation and future implications for cancer immunotherapy. Cancer Immunol. Immunother. 2007, 56:1513-1537.
69. Vodyanik M.A., Bork J.A., Thomson J.A., Slukvin I.I. Human embryonic stem cell-derived CD34+ cells: efficient production in the coculture with OP9 stromal cells and analysis of lymphohematopoietic potential. Blood 2005, 105:617-626.
70. Vremec D., Segura E. The purification of large numbers of antigen presenting dendritic cells from mouse spleen. Methods Mol. Biol. 2013, 960:327-350.
71. Whiteside T.L., Ferris R.L., Szczepanski M., Tublin M., Kiss J., Johnson R., Johnson J.T. Dendritic cell-based autologous tumor vaccines for head and neck squamous cell carcinoma: promise vs reality. Head Neck 2015.
72. Wu L., Liu Y.J. Development of dendritic-cell lineages. Immunity 2007, 26:741-750.
73. Xu Y., Zhan Y., Lew A.M., Naik S.H., Kershaw M.H. Differential development of murine dendritic cells by GM-CSF versus Flt3 ligand has implications for inflammation and trafficking. J. Immunol. 2007, 179:7577-7584.
74. Zeng J., Shahbazi M., Wu C., Toh H.C., Wang S. Enhancing immunostimulatory function of human embryonic stem cell-derived dendritic cells by CD1d overexpression. J. Immunol. 2012, 188:4297-4304.
75. Zhan Y., Carrington E.M., van Nieuwenhuijze A., Bedoui S., Seah S., Xu Y., Wang N., Mintern J.D., Villadangos J.A., Wicks I.P., Lew A.M. GM-CSF increases cross-presentation and CD103 expression by mouse CD8(+) spleen dendritic cells. Eur. J. Immunol. 2011, 41:2585-2595.