Isolation and generation of human dendritic cells

Current Protocols in Immunology

Volumn , Issue SUPPL.99, 2012, Pages

  Nair, Smita ^a   Archer, Gerald E ^a   Tedder, Thomas F ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Antigen presentation; Immune disease; Immunologic study in humans; Immunology; In vitro assays for immune cell function; Innate immunity

Indexed keywords

CD83 ANTIGEN; CYTOKINE; METRIZAMIDE; CANCER VACCINE; TUMOR ANTIGEN;

ARTICLE; B LYMPHOCYTE; CELL DIFFERENTIATION; CELL ISOLATION; CELL MATURATION; CELL SELECTION; CELL SUSPENSION; CRYOPRESERVATION; DENDRITIC CELL; DENSITY GRADIENT CENTRIFUGATION; HUMAN; IMMUNOMAGNETIC SEPARATION; LEUKAPHERESIS; LYMPHOCYTE CULTURE; MONOCYTE; MONONUCLEAR CELL; PRIORITY JOURNAL; T CELL DEPLETION; ANTIGEN PRESENTATION; CELL CULTURE; CELL PROLIFERATION; CELL SEPARATION; CULTURE TECHNIQUE; CYTOLOGY; FLOW CYTOMETRY; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; METHODOLOGY;

ANTIGEN PRESENTATION; ANTIGENS, NEOPLASM; CANCER VACCINES; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CELLS, CULTURED; CYTOKINES; DENDRITIC CELLS; FLOW CYTOMETRY; HUMANS; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE ACTIVATION;

EID: 84875813276     PISSN: 19343671     EISSN: 1934368X     Source Type: Journal    
DOI: 10.1002/0471142735.im0732s99     Document Type: Article

References (65)

1. Castiello, L., Sabatino, M., Jin, P., Clayberger, C., Marincola, F.M., Krensky, A.M., and Stroncek, D.F. 2011. Monocyte-derived DC maturation strategies and related pathways: A transcriptional view. Cancer Immunol. Immunother. 60:457-466.
2. Caux, C., Dezutter-Dambuyant,C., Schmitt, D., and Banchereau, J. 1992. GM-CSF and TNF-a cooperate in the generation of dendritic Langerhans cells. Nature 360:258-261.
3. Egner,W. and Hart, D.N.J. 1995. The phenotype of freshly isolated and cultured human bone marrow allostimulatory cells: Possible heterogeneity in bone marrow dendritic cell populations. Immunology 85:611-620.
4. Egner, W., Prickett, T.C.R., and Hart, D.N.J. 1992. Adhesion molecule expression: A comparison of human blood dendritic cells, monocytes, and macrophages. Transplant. Proc. 24:2318.
5. Egner, W., Andreesen, R., and Hart, D.N.J. 1993a. Allostimulatory cells in fresh human blood: Heterogeneity in antigen-presenting cell populations. Transplantation 56:945-950.
6. Egner, W., McKenzie, J.L., Smith, S.M., Beard, M.E.J., and Hart, D.N.J. 1993b. Identification of potent mixed leukocyte reaction-stimulatory cells in human bone marrow. J. Immunol. 150:3043-3053.
7. Engel, P., Wagner, N., and Tedder, T.F. 1995. CD83 workshop report. In Leukocyte Typing V. White Cell Differentiation Antigens (S.F. Schlossman, L. Boumsell, W. Gilks, J.M. Harlan, T. Kishimoto, C. Morimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds.) pp. 693-695. Oxford University Press, Oxford.
8. Fanger, N.A., Wardwell, K., Shen, L., Tedder, T.F., and Guyre, P.M. 1996. Type I (CD64) and type II (CD32) Fc? receptor-mediated phagocytosis by human blood dendritic cells. J. Immunol. 156:541-548.
9. Feuerstein, B., Berger, T.G., Maczek, C., Roder, C., Schreiner, D., Hirsch, U., Haendle, I., Leisgang, W., Glaser, A., Kuss, O., Diepgen, T.L., Schuler, G., and Schuler-Thurner, B. 2000. A method for the production of cryopreserved aliquots of antigen-preloaded, mature dendritic cells ready for clinical use. J. Immunol.Methods 245:15-29.
10. Freudenthal, P.S. and Steinman, R.M. 1990. The distinct surface of human blood dendritic cells, as observed after an improved isolation method. Proc. Natl. Acad. Sci. U.S.A. 87:7689-7702.
11. Gilboa, E. 2007. DC-based cancer vaccines. J. Clin. Invest. 117:1195-1203.
12. Grage-Griebenow, E., Lorenzen, D., Fetting, R., Flad, H.D., and Ernst, M. 1993. Phenotypical and functional characterization of Fc? receptor I (CD64)-negative monocytes, a minor human monocyte subpopulation with a high accessory and antiviral activity. Eur. J. Immunol. 23:3126-3135.
13. Hart, D.N.J. andMcKenzie, J.L. 1988. Isolation and characterization of human tonsil dendritic cells. J. Exp. Med. 168:157-170.
14. Jonuleit, H., Kuhn, U., Muller, G., Steinbrink, K., Paragnik, L., Schmitt, E., Knop, J., and Enk, A.H. 1997. Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27:3135-3142.
15. Kabel, P.J., De Haan-Meulman, M., Voorbij, H.A.M., Kleingeld, M., Knol, E.F., and Drexhage, H.A. 1989. Accessory cells with the morphology and marker pattern of dendritic cells can be obtained from elutriator-purified blood monocyte fractions. An enhancing effect of metrizamide in this differentiation. Immunobiology 179:395-411.
16. Kalinski, P., Vieira, P.L., Schuitemaker, J.H., de Jong, E.C., and Kapsenberg, M.L. 2001. Prostaglandin E(2) is a selective inducer of interleukin-12 p40 (IL-12p40) production and an inhibitor of bioactive IL-12p70 heterodimer. Blood 97:3466-3469.
17. King, P.D. and Katz, D.R. 1989. Human tonsillar dendritic cell-induced T cell responses: Analysis of molecular mechanisms using monoclonal antibodies. Eur. J. Immunol. 19:581-587.
18. Knight, S.C., Farrant, J., Bryant, A., Edwards, A.J., Burman, S., Lever, A., Clarke, J., and Webster, A.D.B. 1986. Non-adherent, low density cells from human peripheral blood contain dendritic cells and monocytes, both with veiled morphology. Immunology 57:595-603.
19. Lafontaine, M., Landry, D., and Montplaisir, S. 1992. The human thymic dendritic cell phenotype and its modification in culture. Cell. Immunol. 142:238-251.
20. Landi, A., Babiuk, L.A., and van Drunen Littelvan den Hurk, S. 2011. Dendritic cells matured by a prostaglandin E2-containing cocktail can produce high levels of IL-12p70 and are moremature and Th1-biased than dendritic cells treated with TNF-alpha or LPS. Immunobiology 216:649-662.
21. Landry, D., Doyon, L., Poudrier, J., Lafontaine,M., Pelletier, M., and Montplaisir, S. 1990. Accessory function of human thymic dendritic cells in Con A-induced proliferation of autologous thymocyte subsets. J. Immunol. 144:836-843.
22. Lee A.W., Truong, T., Bickham, K., Fonteneau, J.F., Larsson, M., Da Silva, I., Somersan, S., Thomas, E.K., and Bhardwaj, N. 2002. A clinical grade cocktail of cytokines and PGE2 results in uniform maturation of human monocytederived dendritic cells: Implications for immunotherapy. Vaccine 20:A8-A22.
23. Lenz, A., Heine, M., Schuler, G., and Romani, N. 1993. Human and murine dermis contain dendritic cells: Isolation bymeans of a novelmethod and phenotypical and functional characterization. J. Clin. Invest. 92:2587-2596.
24. Macagno, A., Napolitani, G., Lanzavecchia, A., and Sallusto, F. 2007. Duration, combination and timing: The signal integration model of dendritic cell activation. Trends Immunol. 28:227-233.
25. Markowicz, S. and Engleman, E.G. 1990. Granulocyte-macrophage colony-stimulating factor promotes differentiation and survival of human peripheral blood dendritic cells in vitro. J. Clin. Invest. 85:955-961.
26. Michiels, A., Tuyaerts, S., Bonehill, A., Corthals, J., Breckpot, K., Heirman, C., Van Meirvenne, S., Dullaers, M., Allard, S., Brasseur, F., van der Bruggen, P., and Thielemans, K. 2005. Electroporation of immature andmature dendritic cells: Implications for dendritic cell-based vaccines. Gene Ther. 12:772-782.
27. Nair, S.K., Boczkowski, D., Morse, M., Cumming, R.I., Lyerly, H.K., and Gilboa, E. 1998. Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA. Nat. Biotechnol. 16:364-369.
28. Nair, S.K.,Hull, S.,Coleman,D., Gilboa, E., Lyerly, H.K., and Morse, M.A. 1999. Induction of carcinoembryonic antigen (CEA)-specific cytotoxic T-lymphocyte responses in vitro using autologous dendritic cells loaded with CEA peptide or CEA RNA in patients withmetastatic malignancies expressing CEA. Int. J. Cancer 82:121-124.
29. Najar, H.M., Bru-Capdeville, A.C., Gieseler, R.K.H., and Peters, J.H. 1990. Differentiation of human monocytes into accessory cells at serumfree conditions. Eur. J. Immunol. 51:339-346.
30. Nestle, F.O., Zheng, X.-G., Thompson, C.B., Turka, L.A., and Nickoloff, B.J. 1993. Characterization of dermal dendritic cells obtained from normal human skin reveals phenotypic and functionally distinctive subsets. J. Immunol. 151:6535-6545.
31. Nicod, L.P., Lipscomb, M.F.,Weissler, J.C., Lyons, C.R., Alberton, J., and Toews, G.B. 1987. Mononuclear cells in human lung parenchyma: Characterization of a potent accessory cell not obtained by bronchoalveolar lavage. Am. Rev. Respir. Dis. 136:818-823.
32. O'Doherty U., Steinman, R.M., Peng, M., Cameron, P.U., Gezelter, S., Kopeloff, I., Swiggard, W.J., Pope, M., and Bhardwaj, N. 1993. Dendritic cells freshly isolated from human blood express CD4 and mature into typical immunostimulatory dendritic cells after culture in monocyte-conditioned medium. J. Exp. Med. 178:1067-1078.
33. Peters, J.H., Ruppert, J., Gieseler, R.K., Najar, H.M., and Xu, H. 1991. Differentiation of human monocytes into CD14 negative accessory cells: Do dendritic cells derive from the monocyte lineage? Pathobiology 59:122-126.
34. Prickett, T.C.R., McKenzie, J.L., and Hart, D.N.J. 1992. Adhesion molecules on human tonsil dendritic cells. Transplantation 53:483-490.
35. Reid, C.D., Fryer, P.R., Clifford, C., Kirk, A., Tikerpae, J., and Knight, S.C. 1990. Identification of hematopoietic progenitors of macrophages and dendritic Langerhans cells (DL-CFU) in human bone marrow and peripheral blood. Blood 76:1139-1149.
36. Reid, C.D., Stackpoole, A., Meager, A., and Tikerpae, J. 1992. Interactions of tumor necrosis factor with granulocyte-macrophage colonystimulating factor and other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34+ progenitors in human bone marrow. J. Immunol. 149:2681-2688.
37. Romani, N., Lenz, A., Glassel, H., Stossel, H., Stanzl, U., Majdic, O., Fritsch, P., and Schuler, G. 1989. Cultured human Langerhans cells resemble lymphoid dendritic cells in phenotype and function. J. Invest. Dermatol. 93:600-609.
38. Romani, N., Gruner, S., Brang, D., Kampgen, E., Lenz, A., Trockenbacher, B., Konwalinka, G., Fritsch, P.O., Steinman, R.M., and Schuler, G. 1994. Proliferating dendritic cell progenitors in human blood. J. Exp. Med. 180:83-93.
39. Romani, N., Reider, D., Heuer, M., Ebner, S., Kampgen, E., Eibl, B., Niederwieser, D., and Schuler, G. 1996. Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196:137-151.
40. Ruppert, J., Friedrichs, D., Xu, H., and Peters, J.H. 1991. IL-4 decreases the expression of the monocyte differentiation marker CD14, paralleled by an increasing accessory potency. Immunobiology 182:449-464.
41. Santiago-Schwarz, F., Belilos, E.,Diamond, B., and Carsons, S.E. 1992. TNF in combination with GM-CSF enhances the differentiation of neonatal cord blood stem cells into dendritic cells and macrophages. J. Leukocyte Biol. 52:274-281.
42. Santiago-Schwarz, F., Divaris, N., Kay, C., and Carsons, S.E. 1993. Mechanisms of tumor necrosis factor-granulocyte-macrophage colony-stimulating factor-induced dendritic cell development. Blood 82:3019-3028.
43. Schreibelt, G., Tel, J., Sliepen, K.H., Benitez-Ribas, D., Figdor, C.G., Adema, G.J., and de Vries, I.J. 2010. Toll-like receptor expression and function in human dendritic cell subsets: implications for dendritic cell-based anti-cancer immunotherapy. Cancer Immunol. Immunother. 59:1573-1582.
44. Skalova, K., Mollova, K., and Michalek, J. 2010. Human myeloid dendritic cells for cancer therapy: Does maturation matter? Vaccine 28:5153-5160.
45. Spisek, R., Bretaudeau, L., Barbieux, I.,Meflah, K., and Gregoire,M. 2001. Standardized generation of fully mature p70 IL-12 secreting monocytederived dendritic cells for clinical use. Cancer Immunol. Immunother. 50:417-427.
46. Steinman, R.M. 1991. The dendritic cell system and its role in immunogenicity. Annu. Rev. Immunol. 9:271-296.
47. Steinman, R.M. andCohn, Z.A. 1973. Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution. J. Exp. Med. 137:1142-1162.
48. Steinman, R.M. and Banchereau, J. 2007. Taking dendritic cells into medicine. Nature 449:419-426.
49. Szabolcs, P., Moore, M.A.S., and Young, J.W. 1995. Expansion of immunostimulatory dendritic cells among the myeloid progeny of human CD34+ bone marrow precursors culturedwith c-kit ligand, granulocyte-macrophage colony-stimulating factor and TNF-a. J. Immunol. 154:5851-5861.
50. Thomas, R. and Lipsky, P.E. 1994. Human peripheral blood dendritic cell subsets. Isolation and characterization of precursor and mature antigen-presenting cells. J. Immunol. 153:4016-4028.
51. Thomas, R., Davis, L.S., and Lipsky, P.E. 1993. Isolation and characterization of human peripheral blood dendritic cells. J. Immunol. 150:821-834.
52. Thomas, R., Davis, L.S., and Lipsky, P.E. 1994. Rheumatoid synovium is enriched in mature antigen-presenting dendritic cells. J. Immunol. 152:2613-2623.
53. Ueno, H., Schmitt, N., Klechevsky, E., Pedroza-Gonzalez, A., Matsui, T., Zurawski, G., Oh, S., Fay, J., Pascual, V., Banchereau, J., and Palucka, K. 2010. Harnessing human dendritic cell subsets for medicine. Immunol. Rev. 234:199-212.
54. Van Voorhis,W.C., Hair, S.L., Steinman, R.M., and Kaplan, G. 1982. Human dendritic cells: Enrichment and characterization from peripheral blood. J. Exp. Med. 155:1172-1187.
55. Weissman, D., Li, Y., Ananworanich, J., Zhou, L.-J., Adelsberger, J., Tedder, T.F., Baseler,M., and Fauci, A.S. 1995. Three populations of cells with dendritic morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. U.S.A. 92:826-830.
56. Wilson, J., Cullup, H., Laurie, R., Sheng, Y., Palkova, A., Radford, K.J., Dickinson, A.M., Rice, A.M., Hart, D.N.J., and Munster, D.J. 2009. Antibody to the dendritic cell surface activation antigen CD83 prevents acute graftversus-host disease. J. Exp. Med. 206:387-398.
57. Wood, G.S. and Freudenthal, R.S. 1992. CD5 monoclonal antibody reacts with human peripheral blood dendritic cells. Am. J. Path. 141:789-795.
58. Xu, H., Friendrichs, U., Gieseler, R.K.H., Ruppert, J., Ocklind, G., and Peters, J.H. 1992. Human blood dendritic cells exhibit a distinct T-cellstimulatory mechanism and differentiation pattern. Scand. J. Immunol. 36:689-696.
59. Young, J.W. and Steinman, R.M. 1988. Accessory cell requirements for the mixed-leukocyte reaction and polyclonal mitogens, as studied with a new technique for enriching blood dendritic cells. Cell. Immunol. 111:167-182.
60. Zhou, L.-J. and Tedder, T.F. 1995a. Human blood dendritic cells selectively expressCD83, amember of the immunoglobulin superfamily. J. Immunol. 154:3821-3835.
61. Zhou, L.-J. and Tedder, T.F. 1995b. A distinct pattern of cytokine gene expression by human CD83+ blood dendritic cells. Blood 86:3295-3301.
62. Zhou, L.-J. and Tedder, T.F. 1996. CD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells. Proc. Natl. Acad. Sci. U.S.A. 93:2588-2592.
63. Zhou, L.-J., Schwarting, R., Smith, H.M., and Tedder, T.F. 1992.Anovel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells and activated lymphocytes is a new member of the immunoglobulin superfamily. J. Immunol. 149:735-742.
64. Ziegler-Heitbrock, L., Ancuta, P., Crowe, S., Dalod, M., Grau, V., Hart, D.N., Leenen, P.J.M., Liu, Y.-J., MacPherson, G., Randolph, G.J., Scherberich, J., Schmitz, J., Shortman, K., Sozzani, S., Strobl, H., Zembala, M., Austyn, J.M., and Lutz, M.B. 2010. Nomenclature of monocytes and dendritic cells in blood. Blood 116:e74-e80.
65. Zvaifler, N.J., Steinman, R.M., Kaplan, G., Lau, L.L., and Rivelis, M. 1985. Identification of immunostimulatory dendritic cells in the synovial effusions of patients with rheumatoid arthritis. J. Clin. Invest. 76:789-800.