Biofuel production based on carbohydrates from both brown and red macroalgae: Recent developments in key biotechnologies

International Journal of Molecular Sciences

Volumn 17, Issue 2, 2016, Pages

  Kawai, Shigeyuki ^a   Murata, Kousaku ^b  

^a KYOTO UNIVERSITY   (Japan)

^b SETSUNAN UNIVERSITY   (Japan)

Author keywords

3, 6 anhydro L galactose; Agarose; Alginate; Escherichia coli; Ethanol; Macroalgae; Mannitol; Saccharomyces cerevisiae; Sphingomonas sp. A1; Vibrio sp

Indexed keywords

ABC TRANSPORTER; AGAR; ALCOHOL; ALGINIC ACID; CARBOHYDRATE; CARRAGEENAN; LAMINARAN; MANNITOL; MANNITOL DEHYDROGENASE; BIOFUEL;

BIOFUEL PRODUCTION; BROWN MACROALGA; ESCHERICHIA COLI; FERMENTATION; GENE CLUSTER; GENETIC ENGINEERING; MACROALGA; METABOLISM; METABOLOMICS; NONHUMAN; OGATAEA; PROTEIN EXPRESSION; RED MACROALGA; REVIEW; RNA SEQUENCE; SACCHARIFICATION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES PARADOXUS; SPHINGOMONAS; BIOTECHNOLOGY; CARBOHYDRATE METABOLISM; SEAWEED;

BIOFUELS; BIOTECHNOLOGY; CARBOHYDRATE METABOLISM; ETHANOL; FERMENTATION; SEAWEED;

EID: 84957606402     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms17020145     Document Type: Review

References (86)

1. Population Division of the Department of Economic and Social Affairs of the United Nations Secretariat. In World Population Prospects: The 2012 Revision, Highlights and Advance Tables; United Nations: New York, NY, USA, 2013.
2. Adams, J.M., Gallagher, J.A., Donnison, I.S. Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments. J. Appl. Phycol. 2009, 21, 569-574. [CrossRef]
3. Yanagisawa, M., Kawai, S., Murata, K. Strategies for the production of high concentrations of bioethanol from seaweeds: Production of high concentrations of bioethanol from seaweeds. Bioengineered 2013, 4, 224-235. [CrossRef] [PubMed]
4. Borines, M.G., de Leon, R.L., Cuello, J.L. Bioethanol production from the macroalgae Sargassum spp. Bioresour. Technol. 2013, 138, 22-29. [CrossRef] [PubMed]
5. Yanagisawa, M., Nakamura, K., Ariga, O., Nakasaki, K. Production of high concentrations of bioethanol from seaweeds that contain easily hydrolyzable polysaccharides. Process Biochem. 2011, 46, 2111-2116. [CrossRef]
6. Zhu, J.Y., Gleisner, R., Scott, C., Luo, X.L., Tian, S. High titer ethanol production from simultaneous enzymatic saccharification and fermentation of aspen at high solids: A comparison between SPORL and dilute acid pretreatments. Bioresour. Technol. 2011, 102, 8921-8929. [CrossRef] [PubMed]
7. Chen, H.Z., Han, Y.J., Xu, J. Simultaneous saccharification and fermentation of steam exploded wheat straw pretreated with alkaline peroxide. Process Biochem. 2008, 43, 1462-1466. [CrossRef]
8. Varga, E., Klinke, H.B., Reczey, K., Thomsen, A.B. High solid simultaneous saccharification and fermentation of wet oxidized corn stover to ethanol. Biotechnol. Bioeng. 2004, 88, 567-574. [CrossRef] [PubMed]
9. Food and Agriculture Organization. The State of World Fisheries and Aquaculture; Food and Agriculture Organization of the United Nations: Rome, Italy, 2014.
10. Wei, N., Quarterman, J., Jin, Y.S. Marine macroalgae: An untapped resource for producing fuels and chemicals. Trends Biotechnol. 2013, 31, 70-77. [CrossRef] [PubMed]
11. Myklestad, S. β-1,3-Glucans in Diatoms and Brown Seaweeds; Cambridge University Press: Cambridge, UK, 1978.
12. Read, S.M., Currie, G., Bacic, A. Analysis of the structural heterogeneity of laminarin by electrospray-ionisation-mass spectrometry. Carbohydr. Res. 1996, 281, 187-201. [CrossRef]
13. Rinaudo, M. Seaweed polysaccharides. In Comprehensive Glycoscience: From Chemistry to System Biology; Kamerling, J.P., Boons, G.-J., Lee, Y.C., Suzuki, A., Taniguchi, N., Voragen, A.G.J., Eds., Elsevier: Oxford, UK, 2007; Volume 2, pp. 691-735.
14. Black, W.A.P. The seasonal variation in weight and chemical composition of the common British Laminariaceae. J. Mar. Biol. Assoc. UK 1950, 29, 45-72. [CrossRef]
15. Horn, S.J., Aasen, I.M., Østgaard, K. Production of ethanol from mannitol by Zymobacter palmae. J. Ind. Microbiol. Biotechnol. 2000, 24, 51-57. [CrossRef]
16. Larsen, B., Salem, D.M.S.A., Sallam, M.A.E., Mishrikey, M.M., Beltagy, A.I. Characterization of the alginates from algae harvested at the Egyptian Red Sea coast. Carbohydr. Res. 2003, 338, 2325-2336. [CrossRef]
17. Kimura, T., Ueda, K., Kuroda, R., Akao, T., Shinohara, N., Ushirokawa, T., Fukagawa, A., Akimoto, T. The seasonal variation in polysaccharide content of brown alga akamoku Sargassum horneri collected off Oshima Island (Fukuoka Prefecture). Nippon Suisan Gakkaishi 2007, 73, 739-744. [CrossRef]
18. Wargacki, A.J.E., Leonard, M.N., Win, D.D., Regitsky, C.N.S., Santos, P.B., Kim, S.R., Cooper, R.M., Raisner, A., Herman, A.B., Sivitz, A., et al. An engineered microbial platform for direct biofuel production from brown macroalgae. Science 2012, 335, 308-313. [CrossRef] [PubMed]
19. Quain, D.E., Boulton, C.A. Growth and metabolism of mannitol by strains of Saccharomyces cerevisiae. J. Gen. Microbiol. 1987, 133, 1675-1684. [CrossRef] [PubMed]
20. Enquist-Newman, M., Faust, A.M., Bravo, D.D., Santos, C.N., Raisner, R.M., Hanel, A., Sarvabhowman, P., Le, C., Regitsky, D.D., Cooper, S.R., et al. Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. Nature 2014, 505, 239-243. [CrossRef] [PubMed]
21. Chujo, M., Yoshida, S., Ota, A., Murata, K., Kawai, S. Acquisition of the ability to assimilate mannitol by Saccharomyces cerevisiae through dysfunction of the general corepressor Tup1-Cyc8. Appl. Environ. Microbiol. 2015, 81, 9-16. [CrossRef] [PubMed]
22. Lee, H., Schneider, H. Ethanol production from xylitol and some other polyols by Pichia angophorae. Biotechnol. Lett. 1987, 9, 581-584. [CrossRef]
23. Horn, S.J., Aasen, I.M., Ostgaard, K. Ethanol production from seaweed extract. J. Ind. Microbiol. Biotechnol. 2000, 25, 249-254. [CrossRef]
24. Kim, N.J., Li, H., Jung, K., Chang, H.N., Lee, P.C. Ethanol production from marine algal hydrolysates using Escherichia coli KO11. Bioresour. Technol. 2011, 102, 7466-7469. [CrossRef] [PubMed]
25. Ota, A., Kawai, S., Oda, H., Iohara, K., Murata, K. Production of ethanol from mannitol by the yeast strain Saccharomyces paradoxus NBRC 0259. J. Biosci. Bioeng. 2013, 116, 327-332. [CrossRef] [PubMed]
26. Zhao, X.Q., Bai, F.W. Yeast flocculation: New story in fuel ethanol production. Biotechnol. Adv. 2009, 27, 849-856. [CrossRef] [PubMed]
27. Barnett, J.A. The catabolism of acyclic polyols by yeasts. J. Gen. Microbiol. 1968, 52, 131-159. [CrossRef]
28. Perfect, J.R., Rude, T.H., Wong, B., Flynn, T., Chaturvedi, V., Niehaus, W. Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene. J. Bacteriol. 1996, 178, 5257-5262. [PubMed]
29. Treitel, M.A., Carlson, M. Repression by SSN6-TUP1 Is directed by MIG1, a repressor activator protein. Proc. Natl. Acad. Sci. USA 1995, 92, 3132-3136. [CrossRef] [PubMed]
30. Murata, K., Kawai, S., Mikami, B., Hashimoto, W. Superchannel of bacteria: Biological significance and new horizons. Biosci. Biotechnol. Biochem. 2008, 72, 265-277. [CrossRef] [PubMed]
31. Takase, R., Ochiai, A., Mikami, B., Hashimoto, W., Murata, K. Molecular identification of unsaturated uronate reductase prerequisite for alginate metabolism in Sphingomonas sp. A1. Biochim. Biophys. Acta 2010, 1804, 1925-1936. [CrossRef] [PubMed]
32. Preiss, J., Ashwell, G. Alginic acid metabolism in bacteria I. Enzymatic formation of unsaturated oligosac-charides and 4-deoxy-L-erythro-5-hexoseulose uronic acid. J. Biol. Chem. 1962, 237, 309-316. [PubMed]
33. Preiss, J., Ashwell, G. Alginic acid metabolism in bacteria II. The enzymatic reduction of 4-deoxy-L-erythro-5-hexoseulose uronic acid to 2-keto-3-deoxy-D-gluconic acid. J. Biol. Chem. 1962, 237, 317-321. [PubMed]
34. Pouyssegur, J., Stoeber, F. Study of the common degradative pathway of hexuronates in Escherichia coli K 12. Purification, properties and individuality of 2-keto-3-deoxy-D-gluconnokinase. Biochimie 1971, 53, 771-781. [CrossRef]
35. Egan, S.E., Fliege, R., Tong, S., Shibata, A., Wolf, R.E., Jr., Conway, T. Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: Sequence analysis and localization of promoters for the edd-eda operon. J. Bacteriol. 1992, 174, 4638-4646. [PubMed]
36. Takase, R., Mikami, B., Kawai, S., Murata, K., Hashimoto, W. Structure-based conversion of the coenzyme requirement of a short-chain dehydrogenase/reductase involved in bacterial alginate metabolism. J. Biol. Chem. 2014, 289, 33198-33214. [CrossRef] [PubMed]
37. Takeda, H., Yoneyama, F., Kawai, S., Hashimoto, W., Murata, K. Bioethanol production from marine biomass alginate by genetically engineered bacteria. Energy Environ. Sci. 2011, 4, 2575-2581. [CrossRef]
38. Hashimoto, W., Miyake, O., Momma, K., Kawai, S., Murata, K. Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J. Bacteriol. 2000, 182, 4572-4577. [CrossRef] [PubMed]
39. Miyake, O., Hashimoto, W., Murata, K. An exotype alginate lyase in Sphingomonas sp. A1: Overexpression in Escherichia coli, purification, and characterization of alginate lyase IV (A1-IV). Protein Expr. Purif. 2003, 29, 33-41. [CrossRef]
40. Yoon, H.J., Hashimoto, W., Miyake, O., Okamoto, M., Mikami, B., Murata, K. Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp A1 alginate lyases. Protein Expr. Purif. 2000, 19, 84-90. [CrossRef] [PubMed]
41. Murata, K., Inose, T., Hisano, T., Abe, S., Yonemoto, Y., Yamashita, T., Takagi, M., Sakaguchi, K., Kimura, A., Imanaka, T. Bacterial alginate lyase: Enzymology, genetics and application. J. Ferment. Bioeng. 1993, 76, 427-437. [CrossRef]
42. Yonemoto, Y., Murata, K., Kimura, A., Yamaguchi, H., Okayama, K. Bacterial alginate lyase: Characterization of alginate lyase-producing bacteria and purification of the enzyme. J. Ferment. Bioeng. 1991, 72, 152-157. [CrossRef]
43. Momma, K., Mishima, Y., Hashimoto, W., Mikami, B., Murata, K. Direct evidence for Sphingomonas sp A1 periplasmic proteins as macromolecule-binding proteins associated with the ABC transporter: Molecular insights into alginate transport in the periplasm. Biochemistry 2005, 44, 5053-5064. [CrossRef] [PubMed]
44. Momma, K., Okamoto, M., Mishima, Y., Mori, S., Hashimoto, W., Murata, K. A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules. J. Bacteriol. 2000, 182, 3998-4004. [CrossRef] [PubMed]
45. Hashimoto, W., Kawai, S., Murata, K. Bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applications. Bioeng. Bugs 2010, 1, 97-109. [CrossRef] [PubMed]
46. Maruyama, Y., Itoh, T., Kaneko, A., Nishitani, Y., Mikami, B., Hashimoto, W., Murata, K. Structure of a bacterial ABC transporter involved in the import of an acidic polysaccharide alginate. Structure 2015, 23, 1643-1654. [CrossRef] [PubMed]
47. Fujii, M., Yoshida, S., Murata, K., Kawai, S. Regulation of pH attenuates toxicity of a byproduct produced by an ethanologenic strain of Sphingomonas sp. A1 during ethanol fermentation from alginate. Bioengineered 2014, 5, 38-44. [CrossRef] [PubMed]
48. Santos, C.N., Regitsky, D.D., Yoshikuni, Y. Implementation of stable and complex biological systems through recombinase-assisted genome engineering. Nat. Commun. 2013, 4, 2503. [CrossRef] [PubMed]
49. Klemm, P., Hjerrild, L., Gjermansen, M., Schembri, M.A. Structure-function analysis of the self-recognizing Antigen 43 autotransporter protein from Escherichia coli. Mol. Microbiol. 2004, 51, 283-296. [CrossRef] [PubMed]
50. Nielsen, J., Larsson, C., van Maris, A., Pronk, J. Metabolic engineering of yeast for production of fuels and chemicals. Curr. Opin. Biotechnol. 2013, 24, 398-404. [CrossRef] [PubMed]
51. Ochiai, A., Hashimoto, W., Murata, K. A biosystem for alginate metabolism in Agrobacterium tumefaciens strain C58: Molecular identification of Atu3025 as an exotype family PL-15 alginate lyase. Res. Microbiol. 2006, 157, 642-649. [CrossRef] [PubMed]
52. Kado, C.I. Historical account on gaining insights on the mechanism of crown gall tumorigenesis induced by Agrobacterium tumefaciens. Front. Microbiol. 2014, 5. [CrossRef] [PubMed]
53. Kim, H.T., Chung, J.H., Wang, D., Lee, J., Woo, H.C., Choi, I.G., Kim, K.H. Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans 2-40. Appl. Microbiol. Biotechnol. 2012, 93, 2233-2239. [CrossRef] [PubMed]
54. Park, D., Jagtap, S., Nair, S.K. Structure of a PL17 family alginate lyase demonstrates functional similarities among exotype depolymerases. J. Biol. Chem. 2014, 289, 8645-8655. [CrossRef] [PubMed]
55. Wang da, M., Kim, H.T., Yun, E.J., Kim do, H., Park, Y.C., Woo, H.C., Kim, K.H. Optimal production of 4-deoxy-L-erythro-5-hexoseulose uronic acid from alginate for brown macro algae saccharification by combining endo- and exo-type alginate lyases. Bioprocess Biosyst. Eng. 2014, 37, 2105-2111. [CrossRef] [PubMed]
56. Ekborg, N.A., Gonzalez, J.M., Howard, M.B., Taylor, L.E., Hutcheson, S.W., Weiner, R.M. Saccharophagus degradans gen. nov., sp. nov., a versatile marine degrader of complex polysaccharides. Int. J. Syst. Evol. Microbiol. 2005, 55, 1545-1549. [CrossRef] [PubMed]
57. Jagtap, S.S., Hehemann, J.H., Polz, M.F., Lee, J.K., Zhao, H. Comparative biochemical characterization of three exolytic oligoalginate lyases from Vibrio splendidus reveals complementary substrate scope, temperature, and pH adaptations. Appl. Environ. Microbiol. 2014, 80, 4207-4214. [CrossRef] [PubMed]
58. Ochiai, A., Yamasaki, M., Mikami, B., Hashimoto, W., Murata, K. Crystal structure of exotype alginate lyase Atu3025 from Agrobacterium tumefaciens. J. Biol. Chem. 2010, 285, 24519-24528. [CrossRef] [PubMed]
59. Hutcheson, S.W., Zhang, H., Suvorov, M. Carbohydrase systems of Saccharophagus degradans degrading marine complex polysaccharides. Mar. Drugs 2011, 9, 645-665. [CrossRef] [PubMed]
60. Takagi, T., Yokoi, T., Shibata, T., Morisaka, H., Kuroda, K., Ueda, M. Engineered yeast whole-cell biocatalyst for direct degradation of alginate from macroalgae and production of non-commercialized useful monosaccharide from alginate. Appl. Microbiol. Biotechnol. 2015, 18, 1-10. [CrossRef] [PubMed]
61. Wang, T.P., Chang, L.L., Chang, S.N., Wang, E.C., Hwang, L.C., Chen, Y.H., Wang, Y.M. Successful preparation and characterization of biotechnological grade agarose from indigenous Gelidium amansii of Taiwan. Process Biochem. 2012, 47, 550-554. [CrossRef]
62. Duckworth, M., Yaphe, W. The structure of agar. Part I. Fractionation of a complex mixture of polysaccharides. Carbohydr. Res. 1971, 16, 189-197. [CrossRef]
63. Campo, V.L., Kawano, D.F., da Silva, D.B., Carvalho, I. Carrageenans: Biological properties, chemical modifications and structural analysis—A review. Carbohydr. Polym. 2009, 77, 167-180. [CrossRef]
64. Yun, E.J., Lee, S., Kim, H.T., Pelton, J.G., Kim, S., Ko, H.J., Choi, I.G., Kim, K.H. The novel catabolic pathway of 3,6-anhydro-L-galactose, the main component of red macroalgae, in a marine bacterium. Environ. Microbiol. 2015, 17, 1677-1688. [CrossRef] [PubMed]
65. De Ley, J., Doudoroff, M. The metabolism of D-galactose in Pseudomonas saccharophila. J. Biol. Chem. 1957, 227, 745-757. [PubMed]
66. Wong, T.Y., Yao, X.T. The DeLey-Doudoroff pathway of galactose metabolism in Azotobacter vinelandii. Appl. Environ. Microbiol. 1994, 60, 2065-2068. [PubMed]
67. Hong, K.K., Vongsangnak, W., Vemuri, G.N., Nielsen, J. Unravelling evolutionary strategies of yeast for improving galactose utilization through integrated systems level analysis. Proc. Natl. Acad. Sci. USA 2011, 108, 12179-12184. [CrossRef] [PubMed]
68. Ostergaard, S., Olsson, L., Johnston, M., Nielsen, J. Increasing galactose consumption by Saccharomyces cerevisiae through metabolic engineering of the GAL gene regulatory network. Nat. Biotechnol. 2000, 18, 1283-1286. [PubMed]
69. Bro, C., Knudsen, S., Regenberg, B., Olsson, L., Nielsen, J. Improvement of galactose uptake in Saccharomyces cerevisiae through overexpression of phosphoglucomutase: Example of transcript analysis as a tool in inverse metabolic engineering. Appl. Environ. Microbiol. 2005, 71, 6465-6472. [CrossRef] [PubMed]
70. Lee, K.S., Hong, M.E., Jung, S.C., Ha, S.J., Yu, B.J., Koo, H.M., Park, S.M., Seo, J.H., Kweon, D.H., Park, J.C., et al. Improved galactose fermentation of Saccharomyces cerevisiae through inverse metabolic engineering. Biotechnol. Bioeng. 2011, 108, 621-631. [CrossRef] [PubMed]
71. Tschopp, J.F., Emr, S.D., Field, C., Schekman, R. GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae. J. Bacteriol. 1986, 166, 313-318. [PubMed]
72. Holden, H.M., Rayment, I., Thoden, J.B. Structure and function of enzymes of the Leloir pathway for galactose metabolism. J. Biol. Chem. 2003, 278, 43885-43888. [CrossRef] [PubMed]
73. Scott, A., Timson, D.J. Characterization of the Saccharomyces cerevisiae galactose mutarotase/UDP-galactose 4-epimerase protein, Gal10p. FEMS Yeast Res. 2007, 7, 366-371. [CrossRef] [PubMed]
74. Thoden, J.B., Sellick, C.A., Timson, D.J., Reece, R.J., Holden, H.M. Molecular structure of Saccharomyces cerevisiae Gal1p, a bifunctional galactokinase and transcriptional inducer. J. Biol. Chem. 2005, 280, 36905-36911. [CrossRef] [PubMed]
75. Christacos, N.C., Marson, M.J., Wells, L., Riehman, K., Fridovich-Keil, J.L. Subcellular localization of galactose-1-phosphate uridylyltransferase in the yeast Saccharomyces cerevisiae. Mol. Genet. Metab. 2000, 70, 272-280. [CrossRef] [PubMed]
76. Majumdar, S., Ghatak, J., Mukherji, S., Bhattacharjee, H., Bhaduri, A. UDPgalactose 4-epimerase from Saccharomyces cerevisiae. A bifunctional enzyme with aldose 1-epimerase activity. Eur. J. Biochem. 2004, 271, 753-759. [CrossRef] [PubMed]
77. Daugherty, J.P., Kraemer, W.F., Joshi, J.G. Purification and properties of phosphoglucomutase from Fleischmann’s yeast. Eur. J. Biochem. 1975, 57, 115-126. [CrossRef] [PubMed]
78. De Jongh, W.A., Bro, C., Ostergaard, S., Regenberg, B., Olsson, L., Nielsen, J. The roles of galactitol, galactose-1-phosphate, and phosphoglucomutase in galactose-induced toxicity in Saccharomyces cerevisiae. Biotechnol. Bioeng. 2008, 101, 317-326. [CrossRef] [PubMed]
79. Ostergaard, S.; Walloe, K.O., Gomes, S.G., Olsson, L., Nielsen, J. The impact of GAL6, GAL80, and MIG1 on glucose control of the GAL system in Saccharomyces cerevisiae. FEMS Yeast Res. 2001, 1, 47-55. [PubMed]
80. Nehlin, J.O., Carlberg, M., Ronne, H. Control of yeast GAL genes by MIG1 repressor—A transcriptional cascade in the glucose response. EMBO J. 1991, 10, 3373-3377. [PubMed]
81. Yun, E.J., Choi, I.G., Kim, K.H. Red macroalgae as a sustainable resource for bio-based products. Trends Biotechnol. 2015, 33, 247-249. [CrossRef] [PubMed]
82. Park, J.H., Hong, J.Y., Jang, H.C., Oh, S.G., Kim, S.H., Yoon, J.J., Kim, Y.J. Use of Gelidium amansii as a promising resource for bioethanol: A practical approach for continuous dilute-acid hydrolysis and fermentation. Bioresour. Technol. 2012, 108, 83-88. [CrossRef] [PubMed]
83. Kim, H.T., Lee, S., Kim, K.H., Choi, I.G. The complete enzymatic saccharification of agarose and its application to simultaneous saccharification and fermentation of agarose for ethanol production. Bioresour. Technol. 2012, 107, 301-306. [CrossRef] [PubMed]
84. Lee, C.H., Kim, H.T., Yun, E.J., Lee, A.R., Kim, S.R., Kim, J.H., Choi, I.G., Kim, K.H. A novel agarolytic β-galactosidase acts on agarooligosaccharides for complete hydrolysis of agarose into monomers. Appl. Environ. Microbiol. 2014, 80, 5965-5973. [CrossRef] [PubMed]
85. Barbeyron, T., Michel, G., Potin, P., Henrissat, B., Kloareg, B. L-Carrageenases constitute a novel family of glycoside hydrolases, unrelated to that of κ-carrageenases. J. Biol. Chem. 2000, 275, 35499-35505. [CrossRef] [PubMed]
86. Hasunuma, T., Ishii, J., Kondo, A. Rational design and evolutional fine tuning of Saccharomyces cerevisiae for biomass breakdown. Curr. Opin. Chem. Biol. 2015, 29, 1-9. [CrossRef] [PubMed]