Increasing galactose consumption by Saccharomyces cerevisiae through metabolic engineering of the GAL gene regulatory network

Nature Biotechnology

Volumn 18, Issue 12, 2000, Pages 1283-1286

  Ostergaard, Simon ^a   Olsson, Lisbeth ^a   Johnston, Mark ^b   Nielsen, Jens ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

GAL system; Gene regulation; Metabolic engineering; Saccharomyces cerevisiae

Indexed keywords

GAL PROTEIN; GALACTOSE; GENE PRODUCT; UNCLASSIFIED DRUG;

ARTICLE; BIOMASS; CARBOHYDRATE METABOLISM; ENZYME ACTIVATION; ENZYME ACTIVITY; FERMENTATION; FUNGUS MUTANT; GENE CONTROL; GLYCOLYSIS; NONHUMAN; OPERON; PRIORITY JOURNAL; REGULATOR GENE; SACCHAROMYCES CEREVISIAE;

BACTERIAL PROTEINS; GALACTOSE; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; GENETIC ENGINEERING; SACCHAROMYCES CEREVISIAE;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0033664269     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/82400     Document Type: Article

References (30)

1. Lessons from metabolic engineering for functional genomics and drug discovery
2. Cellular and metabolic engineering: An overview
3. Metabolic engineering of Saccharomyces cerevisiae
4. Overproduction of glycolytic enzymes in yeast
5. Enzyme kinetics and metabolic control
6. A strategy for increasing an in vivo flux by genetic manipulations
7. Responses of metabolic systems to large changes in enzyme activities and effectors
8. The cysteine-peptidase bleomycin hydrolase is a member of the galactose regulon in yeast
9. Physiological characterisation in aerobic batch cultivations of Saccharomyces cerevisiae strains harboring the MEL1 gene
10. Autogenous regulation of the Saccharomyces cerevisiae regulatory gene GAL80
11. Induced expression of bacterial β-glucosidase activity in Saccharomyces
12. Alteration of cell population structure due to cell lysis in Saccharomyces cerevisiae cells overexpressing the GAL4 gene
13. Negative effect of the transcriptional activator GAL4
14. A new efficient gene disruption cassette for repeated use in budding yeast
15. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae
16. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae
17. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli
18. Mutational analysis of the gal4-encoded transcriptional regulatory protein of Saccharomyces cerevisiae
19. Improved method for high efficiency transformation of intact yeast cells
20. Nondissociation of GAL4 and GAL80 in vivo after galactose induction
21. Quantitation of putative activator-target affinities predicts transcriptional activating potentials
22. Analysis of the galactose signal transduction pathway in Saccharomyces cerevisiae: Interaction between Gal3p and Gal80p
23. Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiae
24. Ssn6-Tup1 is a general repressor of transcription in yeast
25. Control of yeast GAL genes by MIG1 repressor: A transcriptional cascade in the glucose response
26. Repression by SSN6-TUP1 is directed by MIG1, a repressor/activator protein