Cell-free synthesis and multifold screening of Candida antarctica lipase B (CalB) variants after combinatorial mutagenesis of hot spots

Biotechnology Progress

Volumn 27, Issue 1, 2011, Pages 47-53

  Park, Chang Gil ^a   Kwon, Min A ^b   Song, Jae Kwang ^b   Kim, Dong Myung ^a  

^a Chungnam National University   (South Korea)

^b Korea Research Institute of Chemical Technology   (South Korea)

Author keywords

Cell free protein synthesis; Combinatorial mutagenesis; Expression screening; Lipase

Indexed keywords

96-WELL MICROTITER PLATE; CANDIDA ANTARCTICA LIPASE B; CELL FREE PROTEIN SYNTHESIS; CELL-FREE; CELL-FREE EXPRESSION; COMBINATORIAL MUTAGENESIS; E.COLI CELLS; ENZYMATIC ASSAY; ENZYME MOLECULES; EXPRESSION SCREENING; HOT SPOT; LARGE SCALE PRODUCTIONS; MICROBIAL CELLS; PICHIA PASTORIS; TARGET LOCATION; THERMAL STABILITY; WAX ESTERS;

ASSAYS; CANDIDA; CATALYSTS; COMBINATORIAL OPTIMIZATION; ENZYMES; ESTERIFICATION; ESTERS; INDUSTRIAL APPLICATIONS; ISOMERS; MUTAGENESIS; THERMODYNAMIC STABILITY; YEAST;

GENE EXPRESSION;

PRIMER DNA; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOSYNTHESIS; CANDIDA; CELL FREE SYSTEM; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HYDROLYSIS; METABOLISM; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PICHIA;

BASE SEQUENCE; CANDIDA; CELL-FREE SYSTEM; DNA PRIMERS; ENZYME STABILITY; HYDROLYSIS; LIPASE; MUTAGENESIS; PICHIA;

CANDIDA ANTARCTICA; PICHIA PASTORIS;

EID: 79851476421     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.532     Document Type: Article

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