Heparin and vascular endothelial growth factor loaded poly(l-lactide-co-caprolactone) nanofiber covered stent-graft for aneurysm treatment

Journal of Biomedical Nanotechnology

Volumn 11, Issue 11, 2015, Pages 1947-1960

  Wang, Jing ^a   An, Qingzhu ^b   Li, Dawei ^a   Wu, Tong ^a   Chen, Weiming ^a   Sun, Binbin ^a   EI Hamshary, Hany ^c,d   Al Deyab, Salem S ^c   Zhu, Wei ^b   Mo, Xiumei ^a  

^a DONGHUA UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

^c KING SAUD UNIVERSITY   (Saudi Arabia)

^d Tanta University   (Egypt)

Author keywords

Covered Stent Graft; Heparin; Nanofiber; VEGF

Indexed keywords

CARDIOVASCULAR SYSTEM; CELL ENGINEERING; CELL PROLIFERATION; DEGRADATION; DISEASES; EMULSIFICATION; GRAFTING (CHEMICAL); NANOFIBERS; POLYSACCHARIDES; SCANNING ELECTRON MICROSCOPY; STENTS;

DIGITAL SUBTRACTION ANGIOGRAPHY; ELECTROSPUN NANOFIBERS; ENDOVASCULAR STENT-GRAFT; HEPARIN; LASER SCANNING CONFOCAL MICROSCOPES; STENT GRAFTS; VASCULAR ENDOTHELIAL GROWTH FACTOR; VEGF;

ENDOTHELIAL CELLS;

HEPARIN; MOLECULAR SCAFFOLD; POLITEF; POLY LACTIDE CO CAPROLACTONE NANOFIBER; RECOMBINANT VASCULOTROPIN; UNCLASSIFIED DRUG; VASCULOTROPIN 165; NANOFIBER; POLY(LACTIC ACID-CO-EPSILON-CAPROLACTONE); POLYESTER; VASCULOTROPIN A;

ANIMAL CELL; ANIMAL MODEL; ARTICLE; BARE METAL STENT; BRAIN ARTERY ANEURYSM; CELL CULTURE; CELL PROLIFERATION; CELL STRUCTURE; CONTROLLED STUDY; DEGRADATION; DEVICE SAFETY; DIGITAL SUBTRACTION ANGIOGRAPHY; DRUG RELEASE; ENDOTHELIUM CELL; FOLLOW UP; GUIDING CATHETER; IN VITRO STUDY; NANOEMULSION; NANOFABRICATION; NANOPHARMACEUTICS; NEW ZEALAND WHITE (RABBIT); NONHUMAN; PIG; POLITEF IMPLANT; SACCULAR ANEURYSM; THROMBOCYTE AGGREGATION; VASCULAR PATENCY; ANEURYSM; ANIMAL; BLOOD VESSEL PROSTHESIS; CELL SHAPE; CHEMISTRY; DRUG EFFECTS; RABBIT; STENT; TISSUE ENGINEERING; TISSUE SCAFFOLD;

ANEURYSM; ANIMALS; BLOOD VESSEL PROSTHESIS; CELL PROLIFERATION; CELL SHAPE; CELLS, CULTURED; HEPARIN; NANOFIBERS; POLYESTERS; RABBITS; STENTS; SWINE; TISSUE ENGINEERING; TISSUE SCAFFOLDS; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 84956583062     PISSN: 15507033     EISSN: 15507041     Source Type: Journal    
DOI: 10.1166/jbn.2015.2138     Document Type: Article

References (83)

1. R. A. Hoshi, R. Van Lith, M. C. Jen, J. B. Allen, K. A. Lapidos, and G. Ameer, The blood and vascular cell compatibility of heparin-modified ePTFE vascular grafts. Biomaterials 34, 30 (2013).
2. J. Wang, B. Zhou, X. Gu, M. Li, B. Gu, W. Wang, and Y. Li, Treatment of a canine carotid artery aneurysm model with a biodegradable nanofiber-covered stent: A prospective pilot study. Neurology India 61, 282 (2013).
3. A. Chandra, A. Suliman, and N. Angle, Spontaneous dissection of the carotid and vertebral arteries: The 10-year UCSD experience. Annals of Vascular Surgery 21, 178 (2007).
4. J. Wardlaw and P. White, The detection and management of unrup-tured intracranial aneurysms. Brain 123, 205 (2000).
5. I. Vulev and A. Klepanec, Aneurysm, edited by Y. Murai, Croatia InTech Publishers, Rijeka (2012), Vol. 1, pp. 249-268.
6. D. Angiletta, L. Maiellaro, D. Marinazzo, P. Wiesel, R. Pulli, and G. Regina, Endovascular management of type Ib endoleak complicating a juxtarenal aortic aneurysm previously treated with a multilayer stent. Annals Of Vascular Surgery 28, 5 (2014).
7. Y. D. Cho, H.-S. Kang, J. E. Kim, W.-S. Cho, H.-J. Kwon, H.-S. Koh, and M. H. Han, Modified protection using far proximal portion of self-expandable closed-cell stents for embolization of wide-necked intracranial aneurysms. Neuroradiology 56, 851 (2014).
8. X. H. He, W. T. Li, W. J. Peng, J. P. Lu, Q. Liu, and R. Zhao, Endovascular treatment of posttraumatic carotid-cavernous fistulas and pseudoaneurysms with covered stents. Journal Of Neuroimaging 24, 287 (2014).
9. S. K. Kim, J. Lee, J. R. Duncan, D. D. Picus, M. D. Darcy, and S. Sauk, Endovascular treatment of superior mesenteric artery pseu-doaneurysms using covered stents in six patients. AJR. American Journal of Roentgenology 203, 432 (2014).
10. M.-H. Li, Y.-Q. Zhu, C. Fang, W. Wang, P.-L. Zhang, Y.-S. Cheng, H.-Q. Tan, and J.-B. Wang, The feasibility and efficacy of treatment with a Willis covered stent in recurrent intracranial aneurysms after coiling. American Journal of Neuroradiology 29, 1395 (2008).
11. M.-H. Li, Y.-D. Li, H.-Q. Tan, Q.-Y. Luo, and Y.-S. Cheng, Treatment of distal internal carotid artery aneurysm with the willis covered stent: A prospective pilot study1. Radiology 253, 470 (2009).
12. H.-Q. Tan, M.-H. Li, Y.-D. Li, C. Fang, J.-B. Wang, W. Wang, J. Wang, P.-L. Zhang, and Y.-Q. Zhu, Endovascular reconstruction with the Willis covered stent for the treatment of large or giant intracranial aneurysms. Cerebrovascular Diseases 31, 154 (2010).
13. S. Kawaguchi, T. Sakaki, H. Iwahashi, K. Fujimoto, J.-I. Iida, H. Mishima, and N. Nishikawa, Effect of carotid artery stenting on ocular circulation and chronic ocular ischemic syndrome. Cere-brovascular Diseases 22, 402 (2006).
14. J. H. Yang, H.-Y. Choi, H. S. Nam, S. H. Kim, S. W. Han, and J. H. Heo, Mechanism of infarction involving ipsilateral carotid and posterior cerebral artery territories. Cerebrovascular Diseases 24, 445 (2007).
15. P. Jamshidi, K. Mahmoody, and P. Erne, Covered stents: A review. International Journal of Cardiology 130, 310 (2008).
16. G. J. Laarman, F. Kiemeneij, R. Mueller, G. Guagliumi, M. Cobaugh, P. W. Serruys, and I. I. T. I. Symbiot, Feasibility, safety, and preliminary efficacy of a novel ePTFE-covered self-expanding stent in saphenous vein graft lesions: The Symbiot II trial. Catheter-ization and Cardiovascular Interventions 64, 361 (2005).
17. S. Yamaguchi, T. Asakura, S. Miura, T. Ohki, Y. Kanaoka, H. Ohta, N. Yajima, and M. Nakaya, Nonanastomotic rupture of thoracic aortic Dacron graft treated by endovascular stent graft placement. General Thoracic and Cardiovascular Surgery 61, 414 (2013).
18. H. S. Rangwala, C. N. Ionita, S. Rudin, and R. E. Baier, Partially polyurethane-covered stent for cerebral aneurysm treatment. Journal Of Biomedical Materials Research Part B-Applied Biomaterials 89B, 415 (2009).
19. J. J. Hu, W. C. Chao, P. Y. Lee, and C. H. Huang, Construction and characterization of an electrospun tubular scaffold for small-diameter tissue-engineered vascular grafts: A scaffold membrane approach. J. Mech. Behav. Biomed. Mater. 13, 140 (2012).
20. E. F. J. Chlupac and L. Bacakova, Blood vessel replacement: 50 years of development and tissue engineering paradigms in vascular surgery. Physiol. Res. 58, S119 (2009).
21. M. S. Lord, W. Yu, B. Cheng, A. Simmons, L. Poole-Warren, and J. M. Whitelock, The modulation of platelet and endothelial cell adhesion to vascular graft materials by perlecan. Biomaterials 30, 4898 (2009).
22. T. Chandy, G. S. Das, R. F. Wilson, and G. H. Rao, Use of plasma glow for surface-engineering biomolecules to enhance bloodcompat-ibility of Dacron and PTFE vascular prosthesis. Biomaterials 21, 699 (2000).
23. Z. Zhou and M. E. Meyerhoff, Preparation and characterization of polymeric coatings with combined nitric oxide release and immobilized active heparin. Biomaterials 26, 6506 (2005).
24. R. Hoover, R. Rosenberg, W. Haering, and M. Karnovsky, Inhibition of rat arterial smooth muscle cell proliferation by heparin. II. In vitro studies. Circulation Research 47, 578 (1980).
25. M. Princz and H. Sheardown, Heparin-modified dendrimer cross-linked collagen matrices for the delivery of basic fibroblast growth factor (FGF-2). Journal of Biomaterials Science, Polymer Edition 19, 1201 (2008).
26. O. Larm, R. Larsson, and P. Olsson, A new non-thrombogenic surface prepared by selective covalent binding of heparin via a modified reducing terminal residue. Artificial Cells, Blood Substitutes and Biotechnology 11, 161 (1983).
27. T. Asahara and J. M. Isner, Endothelial progenitor cells for vascular regeneration. Journal of Hematotherapy and Stem Cell Research 11, 171 (2002).
28. T. Asahara, T. Takahashi, H. Masuda, C. Kalka, D. Chen, H. Iwaguro, Y. Inai, M. Silver, and J. M. Isner, VEGF contributes to postnatal neovascularization by mobilizing bone marrow-derived endothelial progenitor cells. The EMBO Journal 18, 3964 (1999).
29. N. Ferrara, Vascular endothelial growth factor and the regulation of angiogenesis. Recent Progress in Hormone Research 55, 15; discussion 35-6 (1999).
30. R. D. Galiano, O. M. Tepper, C. R. Pelo, K. A. Bhatt, M. Callaghan, N. Bastidas, S. Bunting, H. G. Steinmetz, and G. C. Gurtner, Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells. The American Journal of Pathology 164, 1935 (2004).
31. E. Shantsila, T. Watson, and G. Y. Lip, Endothelial progenitor cells in cardiovascular disorders. Journal of the American College of Cardiology 49, 741 (2007).
32. C. Urbich and S. Dimmeler, Endothelial progenitor cells characterization and role in vascular biology. Circulation Research 95, 343 (2004).
33. A. H. Dorafshar, N. Angle, M. Bryer-Ash, D. Huang, M. M. Farooq, H. A. Gelabert, and J. A. Freischlag, Vascular endothelial growth factor inhibits mitogen-induced vascular smooth muscle cell proliferation. Journal of Surgical Research 114, 179 (2003).
34. H. Zhang, X. Jia, F. Han, J. Zhao, Y. Zhao, Y. Fan, and X. Yuan, Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration. Biomaterials 34, 2202 (2013).
35. F. Du, H. Wang, W. Zhao, D. Li, D. Kong, J. Yang, and Y. Zhang, Gradient nanofibrous chitosan/poly varepsilon-caprolactone scaffolds as extracellular microenvironments for vascular tissue engineering. Biomaterials 33, 762 (2012).
36. G. Denas and V. Pengo, Current anticoagulant safety. Expert Opinion on Drug Safety 11, 401 (2012).
37. A. Gomez-Outes, M. L. Suarez-Gea, R. Lecumberri, E. Rocha, C. Pozo-Hernandez, and E. Vargas-Castrillon, New parenteral anticoagulants in development. Therapeutic Advances in Cardiovascular Disease 5, 33 (2011).
38. P. B. Salbach, M. Bruckmann, O. Turovets, J. Kreuzer, W. Kubler, and I. Walter-Sack, Heparin-mediated selective release of hepatocyte growth factor in humans. British Journal Of Clinical Pharmacology 50, 221 (2000).
39. G. Ranieri, R. Patruno, E. Ruggieri, S. Montemurro, P. Valerio, and D. Ribatti, Vascular endothelial growth factor (VEGF) as a target of bevacizumab in cancer: From the biology to the clinic. Current Medicinal Chemistry 13, 1845 (2006).
40. N. Ferrara, Role of vascular endothelial growth factor in Physiologic and Pathologic angiogenesis: Therapeutic implications. Seminars In Oncology 29, 10 (2002).
41. R. K. Jain, Tumor angiogenesis and accessibility: Role of vascular endothelial growth factor. Seminars In Oncology 29, 3 (2002).
42. S. Takeshita, L. Zheng, E. Brogi, M. Kearney, L. Pu, S. Bunting, N. Ferrara, J. Symes, and J. Isner, Therapeutic angiogenesis. A single intraarterial bolus of vascular endothelial growth factor augments revascularization in a rabbit ischemic hind limb model. Journal of Clinical Investigation 93, 662 (1994).
43. Y. Yang, X. Li, S. He, L. Cheng, F. Chen, S. Zhou, and J. Weng, Biodegradable ultrafine fibers with core-sheath structures for protein delivery and its optimization. Polymers for Advanced Technologies 22, 1842 (2011).
44. X. Xu, X. Chen, P. Ma, X. Wang, and X. Jing, The release behavior of doxorubicin hydrochloride from medicated fibers prepared by emulsion-electrospinning. Eur. J. Pharm Biopharm. 70, 165 (2008).
45. W. Ji, Y. Sun, F. Yang, J. J. van den Beucken, M. Fan, Z. Chen, and J. A. Jansen, Bioactive electrospun scaffolds delivering growth factors and genes for tissue engineering applications. Pharm Res. 28, 1259 (2011).
46. Y. Yang, X. Li, M. Qi, S. Zhou, and J. Weng, Release pattern and structural integrity of lysozyme encapsulated in core-sheath structured poly(DL-lactide) ultrafine fibers prepared by emulsion electro-spinning. Eur. J. Pharm Biopharm. 69, 106 (2008).
47. X. Zhao and M. Hadjiargyrou, Induction of cell migration in vitro by an electrospun PDGF-BB/PLGA/PEG-PLA nanofibrous scaffold. Journal of Biomedical Nanotechnology 7, 823 (2011).
48. K. T. Shalumon, S. Sowmya, D. Sathish, K. P. Chennazhi, S. V. Nair, and R. Jayakumar, Effect of incorporation of nanoscale bioactive glass and hydroxyapatite in PCL/chitosan nanofibers for bone and periodontal tissue engineering. Journal of Biomedical Nanotechnology 9, 430 (2013).
49. M. Jaiswal, A. Gupta, A. K. Agrawal, M. Jassal, A. K. Dinda, and V. Koul, Bi-Layer composite dressing of gelatin nanofibrous mat and poly vinyl alcohol hydrogel for drug delivery and wound healing application: In-vitro and in-vivo studies. Journal Of Biomedical Nanotechnology 9, 1495 (2013).
50. Q. Wang, N. Zhang, X. W. Hu, J. H. Yang, and Y. M. Du, Algi-nate/polyethylene glycol blend fibers and their properties for drug controlled release. Journal Of Biomedical Materials Research Part A 82A, 122 (2007).
51. L. M. Jiang, H. Z. Sun, A. L. Yuan, K. Zhang, D. W. Li, C. Li, C. Shi, X. W. Li, K. Gao, C. Y. Zheng, B. Yang, and H. C. Sun, Enhancement of osteoinduction by continual simvastatin release from poly(lactic-co-glycolic acid)-hydroxyapatite-simvastatin nano-fibrous scaffold. Journal of Biomedical Nanotechnology 9, 1921 (2013).
52. T. H. B. Eriksen, E. Skovsen, and P. Fojan, Release of antimicrobial peptides from electrospun nanofibres as a drug delivery system. Journal Of Biomedical Nanotechnology 9, 492 (2013).
53. Y. Dai, J. Niu, J. Liu, L. Yin, and J. Xu, In situ encapsulation of lac-case in microfibers by emulsion electrospinning: Preparation, characterization, and application. Bioresour. Technol. 101, 8942 (2010).
54. X. Li, Y. Su, S. Liu, L. Tan, X. Mo, and S. Ramakrishna, Encapsulation of proteins in poly(L-lactide-co-caprolactone) fibers by emulsion electrospinning. Colloids Surf B Biointerfaces 75, 418 (2010).
55. X. Luo, C. Xie, H. Wang, C. Liu, S. Yan, and X. Li, Antitumor activities of emulsion electrospun fibers with core loading of hydrox-ycamptothecin via intratumoral implantation. Int. J. Pharm. 425, 19 (2012).
56. L. Tian, M. P. Prabhakaran, X. Ding, D. Kai, and S. Ramakrishna, Emulsion electrospun vascular endothelial growth factor encapsulated poly(l-lactic acid-co-s-caprolactone) nanofibers for sustained release in cardiac tissue engineering. Journal of Materials Science 47, 3272 (2011).
57. A. L. Yarin, Coaxial electrospinning and emulsion electrospinning of core-shell fibers. Polymers for Advanced Technologies 22, 310 (2011).
58. K. T. Shalumon, K. P. Chennazhi, S. V. Nair, and R. Jayakumar, Development of small diameter fibrous vascular grafts with outer wall multiscale architecture to improve cell penetration. Journal Of Biomedical Nanotechnology 9, 1299 (2013).
59. L. Jin, T. Wang, M.-L. Zhu, M. K. Leach, Y. I. Naim, J. M. Corey, Z.-Q. Feng, and Q. Jiang, Electrospun fibers and tissue engineering. Journal of Biomedical Nanotechnology 8, 1 (2012).
60. P. R. Sreerekha, D. Menon, S. V. Nair, and K. P. Chennazhi, Fabrication of fibrin based electrospun multiscale composite scaffold for tissue engineering applications. Journal Of Biomedical Nanotechnology 9, 790 (2013).
61. S. J. Xin, X. Y. Li, Q. Wang, R. Huang, X. L. Xu, Z. J. Lei, and H. B. Deng, Novel layer-by-layer structured nanofibrous mats coated by protein films for dermal regeneration. Journal Of Biomedical Nanotechnology 10, 803 (2014).
62. J. B. Lee, H. N. Park, W. K. Ko, M. S. Bae, D. N. Heo, D. H. Yang, and I. K. Kwon, Poly(L-lactic acid)/Hydroxyapatite nanocylinders as nanofibrous structure for bone tissue engineering scaffolds. Journal Of Biomedical Nanotechnology 9, 424 (2013).
63. I. H. L. Pereira, E. Ayres, L. Averous, G. Schlatter, A. Hebraud, A. C. C. de Paula, P. H. L. Viana, A. M. Goes, and R. L. Orefice, Differentiation of human adipose-derived stem cells seeded on mineralized electrospun co-axial poly(epsilon-caprolactone) (PCL)/gelatin nanofibers. Journal Of Materials Science-Materials In Medicine 25, 1137 (2014).
64. K. E. Park, H. K. Kang, S. J. Lee, B. M. Min, and W. H. Park, Biomimetic nanofibrous scaffolds: PreparatiC o on and characterizatr i ion of PGA/chitin blend nanofibers. Biomacromolecules 7, 635 (2006).
65. J. P. Chen, G. Y. Chang, and J. K. Chen, Electrospun colla-gen/chitosan nanofibrous membrane as wound dressing. Colloids And Surfaces A-Physicochemical and Engineering Aspects 313, 183 (2008).
66. F. Y Ding, H. B. Deng, Y. M. Du, X. W Shi, and Q. Wang, Emerging chitin and chitosan nanofibrous materials for biomedical applications. Nanoscale 6, 9477 (2014).
67. C. Wu, Q. An, D. Li, J. Wang, L. He, C. Huang, Y. Li, W Zhu, and X. Mo, A novel heparin loaded poly(l-lactide-co-caprolactone) covered stent for aneurysm therapy. Materials Letters 116, 39 (2014).
68. Z.-M. Huang, Y. Zhang, S. Ramakrishna, and C. Lim, Electrospinning and mechanical characterization of gelatin nanofibers. Polymer 45, 5361 (2004).
69. S. Yan, L. Xiaoqiang, T. Lianjiang, H. Chen, and M. Xiumei, Poly(l-lactide-co-s-caprolactone) electrospun nanofibers for encapsulating and sustained releasing proteins. Polymer 50, 4212 (2009).
70. K. Wang, Q. Huang, B. Hong, Y. Xu, W Zhao, J. Chen, R. Zhao, and J. Liu, Neck injury is critical to elastase-induced aneurysm model. American Journal of Neuroradiology 30, 1685 (2009).
71. R. B. M. J. B. Wissink, J. S. Pieper, A. A. Poot, G. H. M. Engbers, T. Beugelin, W G. van Aken, and J. Feijen, Immobilization of heparin to EDC/NHS-crosslinked collagen characterization and in vitro evaluation. Biomaterials 22, 151 (2001).
72. L. Liu, S. Guo, J. Chang, C. Ning, C. Dong, and D. Yan, Surface modification of polycaprolactone membrane via layer-by-layer deposition for promoting blood compatibility. J. Biomed. Mater. Res. B Appl. Biomater 87, 244 (2008).
73. P. Y. Tseng, S. S. Rele, X. L. Sun, and E. L. Chaikof, Membrane-mimetic films containing thrombomodulin and heparin inhibit tissue factor-induced thrombin generation in a flow model. Biomaterials 27, 2637 (2006).
74. X. Jia, C. Zhao, P. Li, H. Zhang, Y. Huang, H. Li, J. Fan, W Feng, X. Yuan, and Y. Fan, Sustained release of VEGF by coaxial electrospun dextran/PLGA fibrous membranes in vascular tissue engineering. Journal of Biomaterials Science, Polymer Edition 22, 1811 (2011).
75. S. I. Jeong, B. S. Kim, S. W. Kang, J. H. Kwon, Y. M. Lee, S. H. Kim, and Y. H. Kim, In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-epsilon-caprolactone) scaffolds. Biomaterials 25, 5939 (2004).
76. X. M. Mo, C. Y. Xu, M. Kotaki, and S. Ramakrishna, Electrospun P(LLA-CL) nanofiber: A biomimetic extracellular matrix for smooth muscle cell and endothelial cell proliferation. Biomaterials 25, 1883 (2004).
77. X. Li, Y. Su, X. Zhou, and X. Mo, Distribution of Sorbitan Monooleate in poly(l-lactide-co-s-caprolactone) nanofibers from emulsion electrospinning. Colloids and Surfaces B: Biointerfaces 69, 221 (2009).
78. L. Wu, H. Li, S. Li, X. Li, X. Yuan, X. Li, and Y. Zhang, Composite fibrous membranes of PLGA and chitosan prepared by coelectrospin-ning and coaxial electrospinning. J. Biomed. Mater. Res. A 92, 563 (2010).
79. D. B. Pike, S. S. Cai, K. R. Pomraning, M. A. Firpo, R. J. Fisher, X. Z. Shu, G. D. Prestwich, and R. A. Peattie, Heparin-regulated release of growth factors in vitro and angiogenic response in vivo to implanted hyaluronan hydrogels containing VEGF and bFGF. Biomaterials 27, 5242 (2006).
80. X. W. Y. Z. Zhang, Y. Feng, J. Li, C. T. Lim, and S. Ramakrishna, Coaxial electrospinning of (fluorescein isothiocyanate-conjugated bovine serum albumin)-encapsulated poly(e-caprolactone) nanofibers for sustained release. Biomacromolecules 7, 1049 (2006).
81. K. Zhang, A. Yin, C. Huang, C. Wang, X. Mo, S. S. Al-Deyab, and M. El-Newehy, Degradation of electrospun SF/P(LLA-CL) blended nanofibrous scaffolds in vitro. Polymer Degradation and Stability 96, 2266 (2011).
82. J. Wu, C. Huang, W. Liu, A. Yin, W. Chen, C. He, H. Wang, S. Liu, C. Fan, G. L. Bowlin, and X. Mo, Cell infiltration and vascularization in porous nanoyarn scaffolds prepared by dynamic liquid electrospinning. Journal of Biomedical Nanotechnology 10, 603 (2014).
83. M. I. Santos, K. Tuzlakoglu, S. Fuchs, M. E. Gomes, K. Peters, R. E. Unger, E. Piskin, R. L. Reis, and C. J. Kirkpatrick, Endothelial cell colonization and angiogenic potential of combined nano-and micro-fibrous scaffolds for bone tissue engineering. Biomaterials 29, 4306 (2008).