DNA affinity capturing identifies new regulators of the heterologously expressed novobiocin gene cluster in Streptomyces coelicolor M512

Applied Microbiology and Biotechnology

Volumn 100, Issue 10, 2016, Pages 4495-4509

  Bekiesch, Paulina ^a,b   Franz Wachtel, Mirita ^a   Kulik, Andreas ^a   Brocker, Melanie ^c   Forchhammer, Karl ^a   Gust, Bertolt ^a,b   Apel, Alexander Kristian ^a,b  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b German Centre for Infection Research (DZIF)   (Germany)

^c FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

DNA affinity capturing assay; Heterologous expression; Novobiocin; Regulation; Streptomyces; Surface plasmon resonance

Indexed keywords

BACTERIA; BIOSYNTHESIS; DNA; MASS SPECTROMETRY; PLASMONS; PROTEINS; SURFACE PLASMON RESONANCE;

DNA AFFINITY; HETEROLOGOUS EXPRESSION; NOVOBIOCIN; REGULATION; STREPTOMYCES;

GENES;

ADPA PROTEIN; DNA; DNA BINDING PROTEIN; NDGR PROTEIN; NOVOBIOCIN; REGULATOR PROTEIN; SLBR PROTEIN; UNCLASSIFIED DRUG; WHIA PROTEIN; BACTERIAL DNA; BACTERIAL PROTEIN; CULTURE MEDIUM; GLUTATHIONE TRANSFERASE; HRDB PROTEIN, STREPTOMYCES; SIGMA FACTOR;

ANTIBIOTICS; BACTERIUM; BIOASSAY; CHEMICAL BINDING; DNA; GENE EXPRESSION; GENETIC ANALYSIS; MUTATION; PROTEIN;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; DNA AFFINITY CAPTURING ASSAY; DNA DETERMINATION; GENE CLUSTER; GENE DELETION; GENE OVEREXPRESSION; HETEROLOGOUS EXPRESSION; LIQUID CHROMATOGRAPHY; MUTAGENESIS; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; STREPTOMYCES; STREPTOMYCES COELICOLOR; STREPTOMYCES NIVEUS; SURFACE PLASMON RESONANCE; TANDEM MASS SPECTROMETRY; BACTERIAL GENE; BIOSYNTHESIS; CULTURE MEDIUM; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MULTIGENE FAMILY; PLASMID;

STREPTOMYCES; STREPTOMYCES CAERULEUS; STREPTOMYCES COELICOLOR;

BACTERIAL PROTEINS; CULTURE MEDIA; DNA, BACTERIAL; DNA-BINDING PROTEINS; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GLUTATHIONE TRANSFERASE; MULTIGENE FAMILY; NOVOBIOCIN; PLASMIDS; PROMOTER REGIONS, GENETIC; SIGMA FACTOR; STREPTOMYCES COELICOLOR;

EID: 84955249900     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-016-7306-1     Document Type: Article

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