Tree diversity and species identity effects on soil fungi, protists and animals are context dependent

ISME Journal

Volumn 10, Issue 2, 2016, Pages 346-362

  Tedersoo, Leho ^a   Bahram, Mohammad ^b   Cajthaml, Tomáš ^c,d   Põlme, Sergei ^a   Hiiesalu, Indrek ^b   Anslan, Sten ^b   Harend, Helery ^b   Buegger, Franz ^e   Pritsch, Karin ^e   Koricheva, Julia ^f   Abarenkov, Kessy ^a  

^a UNIVERSITY OF TARTU   (Estonia)

^b UNIVERSITY OF TARTU   (Estonia)

^c INSTITUTE OF MICROBIOLOGY   (Czech Republic)

^d CHARLES UNIVERSITY   (Czech Republic)

^e INSTITUTE OF EPIDEMIOLOGY   (Germany)

^f UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOCORRELATION; BIOMASS; ECOSYSTEM FUNCTION; ENVIRONMENTAL EFFECT; EUKARYOTE; FOREST ECOSYSTEM; FUNGUS; GENETIC ANALYSIS; PLANT COMMUNITY; PROTIST; SOIL BIOTA; SOIL-VEGETATION INTERACTION; SPECIES RICHNESS;

ESTONIA; FINLAND;

ANIMALIA; EUKARYOTA; FUNGI; PROTISTA;

SOIL;

ANIMAL; BIODIVERSITY; BIOMASS; BIOTA; CHEMISTRY; CLASSIFICATION; EUKARYOTE; FINLAND; FUNGUS; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; PARASITOLOGY; SOIL; TREE;

ANIMALS; BIODIVERSITY; BIOMASS; BIOTA; EUKARYOTA; FINLAND; FUNGI; SOIL; SOIL MICROBIOLOGY; TREES;

EID: 84955067712     PISSN: 17517362     EISSN: 17517370     Source Type: Journal    
DOI: 10.1038/ismej.2015.116     Document Type: Article

References (112)

1. Abarenkov K, Nilsson RH, Larsson K-H, Alexander IJ, Eberhardt U, Erland S et al. (2010a). The UNITE database for molecular identification of fungi-recent updates and future perspectives. New Phytol 186: 281-285.
2. Abarenkov K, Tedersoo L, Nilsson RH, Vellak K, Saar I, Veldre V et al. (2010b). PlutoF - A web based workbench for ecological and taxonomic research with an online implementation for fungal ITS sequences. Evol Bioinform Online 6: 189-196.
3. Adl SM, Gupta VVSR. (2006). Protists in soil ecology and forest nutrient cycling. Can J For Res 36: 1805-1817.
4. Ampoorter E, Baeten L, Koricheva J, Vanhellemont M, Verheyen K. (2014). Do diverse overstoreys induce diverse understoreys? Lessons learnt from an experimental-observational platform in Finland. Forest Ecol Manag 318: 206-215.
5. Anderson MJ. (2005). PERMANOVA: A FORTRAN Computer Program for Permutational Multivariate Analysis of Variance. University of Auckland: Auckland, New Zealand.
6. Anslan S, Tedersoo L. (2015). Performance of cytochrome c oxidase subunit I (COI), ribosomal DNA Large Subunit (LSU) and Internal Transcribed Spacer 2 (ITS2) in DNA barcoding of Collembola. Eur J Soil Biol 69: 1-7.
7. Aponte C, García LV, Marañón T. (2013). Tree species effects on nutrient cycling and soil biota: A feedback mechanism favouring species coexistence. Forest Ecol Manag 309: 36-46.
8. Arif C, Daniels C, Bayer T, Banguera-Hinestroza E, Barbrook A, Howe CJ et al. (2014). Assessing Symbiodinium diversity in scleractinian corals via nextgeneration-based genotyping of the ITS2 rDNA region. Mol Ecol 23: 4418-4433.
9. Ayres E, Steltzer H, Berg S, Wallenstein MD, Simmons BL, Wall DH. (2009). Tree species traits influence soil physical, chemical, and biological properties in high elevation forests. PLoS One 4: e5964.
10. Bachy C, Dolan JR, Lopez-Garcia P, Deschamps P, Moreira D. (2013). Accuracy of protist diversity assessments: morphology compared with cloning and direct pyrosequencing of 18 S rRNA genes and ITS regions using the conspicuous tintinnid ciliates as a case study. ISME J 7: 244-255.
11. Bagchi R, Gallery RE, Gripenberg S, Gurr SJ, Narayan L, Addis CE et al. (2014). Pathogens and insect herbivores drive rainforest plant diversity and composition. Nature 506: 85-88.
12. Bahram M, Kõljalg U, Courty P-E, Diedhiou A, Kjøller R, Põlme S et al. (2013). The distance-decay of similarity in communities of ectomycorrhizal fungi in different ecosystems and scales. J Ecol 101: 1335-1344.
13. Bahram M, Põlme S, Kõljalg U, Zarre S, Tedersoo L. (2012). Regional and local patterns of ectomycorrhizal fungal diversity and community structure along an altitudinal gradient in the Hyrcanian forests of northern Iran. New Phytol 193: 465-473.
14. Baldrian P, Kolarik M, Stursova M, Kopecky J, Valaskova V, Vetrovsky T et al. (2012). Active and total microbial communities in forest soil are largely different and highly stratified during decomposition. ISME J 6: 248-258.
15. Baldwin DS, Colloff MJ, Rees GN, Chariton AA, Watson GO, Court LN et al. (2013). Impacts of inundation and drought on eukaryote biodiversity in semi-arid floodplain soils. Mol Ecol 22: 1746-1758.
16. Bates ST, Clemente JC, Flores GE, Walters WA, Wegener Parfrey L et al. (2013). Global biogeography of highly diverse protistan communities in soil. ISME J 7: 652-659.
17. Baxter JW, Dighton J. (2005). Phosphorus source alters host plant response to ectomycorrhizal diversity. Mycorrhiza 15: 513-523.
18. Bengtsson-Palme J, Ryberg M, Hartmann M, Branco S, Wang Z, Godhe A et al. (2013). Improved software detection and extraction of ITS1 and ITS2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing data. Meth Ecol Evol 4: 914-919.
19. Benjamini Y, Hochberg Y. (2000). On the adaptive control of the false discovery rate in multiple testing with independent statistics. J Educ Behav Stat 25: 60-83.
20. Bik HM, Porazinska D, Creer S, Caporaso JG, Knight R, Thomas WK. (2012). Sequencing our way towards understanding global eukaryotic biodiversity. Trends Ecol Evol 27: 233-243.
21. Bligh EG, Dyer WJ. (1959). A rapid method of total lipid extraction and purification. Can J Biochem Phys 37: 911-917.
22. Borer ET, Seabloom EW, Tilman D. (2012). Plant diversity contrls arthropod biomass and temporal stability. Ecol Lett 15: 1457-1464.
23. Bragg L, Stone G, Imelfort M, Hugenholtz P, Tyson GW. (2012). Fast, accurate error-correction of amplicon pyrosequences using Acacia. Nat Methods 9: 425-426.
24. Bruelheide H, Nadrowski K, Assmann T, Bauhus J, Both S, Buscot F et al. (2014). Designing forest biodiversity experiments: general considerations. illustrated by a new large experiment in subtropical China. Methods Ecol Evol 5: 74-89.
25. Cardinale BJ, Matulich KL, Hooper DU, Byrnes JE, Duffy E, Gamfeldt L et al. (2011). The functional role of producer diversity in ecosystems. Am J Bot 98: 572-592.
26. Cardinale BJ, Srivastava DS, Duffy JE, Wright JP, Downing AL, Sankaran M et al. (2006). Effects of biodiversity on the functioning of trophic groups and ecosystems. Nature 443: 989-992.
27. Chariton AA, Court LN, Hartley DM, Coloff MJ, Hardy CM. (2010). Ecological assessment of estuarine sediments by pyrosequencing eukaryotic ribosomal DNA. Front Ecol 8: 233-238.
28. Clemmensen KE, Bahr A, Ovaskainen O, Dahlberg A, Ekblad A, Wallander H et al. (2013). Roots and associated fungi drive long-term carbon sequestration in boreal forest. Science 339: 1615-1618.
29. Coleman AW. (2009). Is there a molecular key to the level of 'biological species' in eukaryotes? A DNA guide. Mol Phyl Evol 50: 197-203.
30. Creer S, Fonseca VG, Porazinska DL, Giblin-Davis RM, Sung W, Power DM et al. (2010). Ultrasequencing of the meiofaunal biosphere: practice, pitfalls and promises. Mol Ecol 19: S4-S20.
31. Deagle BE, Jarman SN, Coissac E, Pompanon F, Taberlet P. (2014). DNA metabarcoding and the cytochrome c oxidase subunit I marker: not a perfect match. Biol Lett 10: 20140562.
32. de Barba M, Boyer MF, Rioux MD, Coissac E, Taberlet P. (2014). DNA metabarcoding multiplexing and validation of data accuracy for diet assessment: Application to omnivorous diet. Mol Ecol Res 14: 306-323.
33. de Wit P, Erseus C. (2010). Genetic variation and phylogeny of Scandinavian species of Grania (Annelida, Clitellata, Enchytraediae), with discovery of a cryptic species. J Zool Syst Evol Res 48: 285-293.
34. Edgar RC, Haas BJ, Clemente JC, Quince C, Knight R. (2011). UCHIME improves sensitivity and speed of chimera detection. Bioinformatics 27: 2194-2200.
35. Eisenhauer N, Dobies T, Cesarz S, Hobbie SE, Worm K, Reich PB et al. (2013). Plant diversity effects on soil food webs are stronger than those of elevated CO2 and N deposition in a long-term grassland experiment. Proc Natl Acad Sci USA 17: 6889-6894.
36. Fierer N, Jackson JA, Vilgalys R, Jackson RB. (2005). Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Appl Environ Microbiol 71: 4117-4120.
37. Frouz J, Liveckova M, Albrechtova J, Chronakova A, Cajthaml T, Pizl V et al. (2013). Is the effect of trees on soil properties mediated by soil fauna? A case study from post-mining sites. For Ecol Manage 309: 87-95.
38. Fu L, Niu B, Zhu Z, Wu S, Li W. (2012). CD-HIT: Accelerated for clustering the next-generation sequencing data. Bioinformatics 23: 3150-3152.
39. Gamfeldt L, Snäll T, Bagchi R, Jonsson M, Gustafsson L, Kjellander P et al. (2013). Higher levels of multiple ecosystem services are found in forests with more tree species. Nat Commun 4: 1340.
40. Hajibabaei M, Shokralla S, Zhou X, Singer GC, Baird DJ. (2011). Environmental barcoding: A next-generation sequencing approach for biomonitoring applications using river benthos. PLoS One 6: e17497.
41. Healy C, Gotelli NJ, Potvin C. (2008). Partitioning the effects of biodiversity and environmental heterogeneity for productivity and mortality in a tropical tree plantation. J Ecol 96: 903-913.
42. Hiiesalu I, Pärtel M, Davison J, Gerhold P, Metsis M, MooraM et al. (2014). Species richness of arbuscular mycorrhizal fungi: Associations with grassland plant richness and biomass. New Phytol 203: 233-234.
43. Hooper DU, Adair EC, Cardinale BJ, Byrnes JEK, Hungate BA, Matulich KA et al. (2012). A global synthesis reveals biodiversity loss as a major driver of ecosystem change. Nature 486: 105-108.
44. Huston MA. (1997). Hidden treatments in ecological experiments: re-evaluating the ecosystem function of biodiversity. Oecologia 110: 449-460.
45. Jumpponen A, Jones KL. (2009). Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperate Quercus macrocarpa phyllosphere. New Phytol 184: 438-448.
46. Katoh K, Standley DM. (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30: 772-780.
47. Koetschan C, Förster F, Keller A, Schleicher T, Ruderisch B, Schwarz R et al. (2010). The ITS2 Database III-sequences and structures for phylogeny. Nucleic Acid Res 38: D275-D279.
48. Kõljalg U, Nilsson RH, Abarenkov K, Tedersoo L, Taylor AFS, Bahram M et al. (2013). Towards a unified paradigm for sequence-based identification of Fungi. Mol Ecol 22: 5271-5277.
49. Lauber C, Hamady M, Knight R, Fierer N. (2009). Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale. Appl Environ Microbiol 75: 5111-5120.
50. Leake J, Johnson D, Donnelly D, Muckle G, Boddy L, Read DJ. (2004). Networks of power and influence: The role of mycorrhizal mycelium in controlling plant communities and agroecosystem functioning. Can J Bot 82: 1016-1045.
51. Legendre P. (2008). Studying beta diversity: ecological variation partitioning by multiple regression and canonical analysis. J Plant Ecol 1: 3-8.
52. Lentendu G, Wubet T, Chatzinotas A, Wilhelm C, Buscot F, Schlegel M. (2014). Effects of long-term differential fertilization on eukaryotic microbial communities in an arable soil: A multiple barcoding approach. Mol Ecol 23: 3341-3355.
53. Lindahl BD, Nilsson RH, Tedersoo L, Abarenkov K, Carlsen T, Kjøller R et al. (2013). Fungal community analysis by high-throughput sequencing of amplified markers - A user's guide. New Phytol 199: 288-299.
54. Lisboa FJG, Peres-Neto PR, Chaer GM, EdC Jesus, Mitchell RJ et al. (2014). Much beyond Mantel: bringing procrustes association metric to the plant and soil ecologist's toolbox. PLoS One 9: e101238.
55. Litaker RW, VanderseaMW, Kibler SR, Reece KS, Stokes NA, Lutzoni FM et al. (2007). Recognizing dinoflagellate species using ITS rDNA sequences. J Phycol 43: 344-355.
56. Lundberg DS, Yourstone S, Mieczkowski P, Jones CD, Dangl JL. (2013). Practical innovations for highthroughput amplicon sequencing. Nat Methods 10: 999-1002.
57. Magurran AE. (1988). Ecological Diversity and its Measurement. Princeton University Press: Princeton, NJ, USA.
58. Maherali H, Klironomos JN. (2007). Influence of phylogeny on fungal community assembly and ecosystem functioning. Science 316: 1746-1748.
59. Medinger R, Nolte V, Pandey RM, Jost S, Ottenwälder B, Schlötterer C et al. (2010). Diversity in a hidden world: potential and limitation of next-generation sequencing for surveys of molecular diversity of eukaryotic microorganisms. Mol Ecol 19: 32-40.
60. Moon-van der Staay SY, De Vachter R, Vaulot D. (2001). Oceanic 18 S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity. Nature 409: 607-610.
61. Nadrowski K, Wirth C, Scherer-Lorenzen M. (2010). Is forest diversity driving ecosystem function and service? Curr Opin Environ Sustain 2: 75-79.
62. Noge K, Mori N, Tanaka C, Nishida R, Tsuda M, Kuwahara Y. (2005). Identification of astigmatid mites using the second internal transcribed spacer (ITS2) region and its application for phylogenetic study. Exp Appl Acarol 35: 29-46.
63. Nuismer SL, Jordano P, Bascompte J. (2013). Coevolution and the architecture of mutualistic networks. Evolution 67: 338-354.
64. Pärtel M. (2002). Local plant diversity patterns and evolutionary history at the regional scale. Ecology 83: 2361-2366.
65. Pawlowski J, Audic S, Adl S. (2012). CBOL Protist Working Group: barcoding eukaryotic richness beyond the animal, plant, and fungal kingdoms. PLoS Biol 10: e1001419.
66. Peay KG, Baraloto C, Fine PVA. (2013). Strong coupling of plant and fungal community structure across western Amazonian rainforests. ISME J 7: 1852-1861.
67. Pellissier L, Niculita-Hirzel H, Dubuis A, Pagni M, Guex N, Ndiribe C et al. (2014). Soil fungal communities in grasslands are environmentally structured at a regional scale in the Alps. Mol Ecol 23: 4274-4290.
68. Põlme S, Bahram M, Kõljalg U, Tedersoo L. (2014). Global biogeography of Alnus-associated Frankia Actinobacteria. New Phytol 204: 979-988.
69. Põlme S, BahramM, Yamanaka T, Nara K, DaiYC, GrebencT et al. (2013). Biogeography of ectomycorrhizal fungi associated with alders (Alnus spp. ) in relation to biotic and abiotic variables at the global scale. New Phytol 198: 1239-1249.
70. Porazinska DL, Bardgett RD, Blaauw MB, Hunt HW, Parsons AN, Seastedt TR et al. (2003). Relationships at the aboveground-belowground interface: plants, soil biota and soil processes. Ecol Monogr 73: 377-395.
71. Porazinska DL, Giblin-Davis RM, Faller L, Farmerie W, Kanzaki N, Morris K et al. (2009). Evaluating highthroughput sequencing as a method for metagenomic analysis of nematode diversity. Mol Ecol Res 9: 1439-1450.
72. Prescott CE, Grayston SJ. (2013). Tree species influence on microbial communities in litter and soil: current knowledge and research needs. For Ecol Manage 309: 19-27.
73. R Core Development Team (2014). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing: Vienna, Austria. Available from http://www. R-project. org/.
74. Ramirez KS, Leff JW, Barberan A, Bates ST, Betley J, Crowther TW et al. (2014). Biogeographic patterns in below-ground diversity in New York City's Central Park are similar to those observed globally. Proc R Soc B 281: 20141988.
75. Read D, Leake JR, Perez-Moreno J. (2004). Mycorrhizal fungi as drivers of ecosystem processes in heathland and boreal forest biomes. Can J Bot 82: 1243-1263.
76. Reich PB, Tilman D, Isbell F, Mueller K, Hobbie SE, Flynn DFB et al. (2012). Impacts of biodiversity loss escalate through time as redundancy fades. Science 336: 589-592.
77. Rousk J, Baath E, Brokes PC, Lauber CL, Lozupone C, Caporaso JG et al. (2010). Soil bacterial and fungal communities across a pH gradient in an arable soil. ISME J 4: 1340-1351.
78. Scherber C, Eisenhauer N, WeisserWW, Schmid B, Voigt W, Fischer M et al. (2010). Bottom-up levels of plant diversity on multitrophic interactions in a biodiversity experiment. Nature 468: 553-556.
79. Scherer-Lorenzen M, Potvin C, Koricheva J, Schmid B, Hector A, Bornik Z et al. (2006). The design of experimental tree plantations for functional biodiversity research. Ecol Stud 176: 348-375.
80. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA et al. (2012). Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA 109: 6241-6246.
81. Serna-Chavez H, Fierer N, van Bodegom PM. (2013). Global drivers and patterns of microbial abundance in soil. Glob Ecol Biogeogr 10: 1162-1172.
82. Smith DP, Peay KG. (2014). Sequence depth, not PCR replication, improves ecological inference from next generation DNA sequencing. PLoS One 9: e90234.
83. Šnajdr J, Cajthaml T, Valášková V, Merhautová V, Petranková M, Spetz P et al. (2011). Transformation of Quercus petraea litter: successive changes in litter chemistry are reflected in differential enzyme activity and changes in the microbial community composition. FEMS Microbiol Ecol 75: 291-303.
84. Šnajdr J, Valášková V, Merhautová V, Herinková J, Cajthaml T, Baldrian P. (2008). Spatial variability of enzyme activities and microbial biomass in the upper layers of Quercus petraea forest soil. Soil Biol Biochem 40: 2068-2075.
85. Soininen EM, Zinger L, Gielly L, Bellemain E, Bråthen KA, Brochmann C et al. (2013). Shedding new light on the diet of Norwegian lemmings: DNA metabarcoding of stomach content. Polar Biol 36: 1069-1076.
86. Soininen J. (2014). A quantitative analysis of species sorting across organisms and ecosystems. Ecology 95: 3284-3292.
87. Steudel B, Hector A, Friedl T, Löfke C, Lorenz M, WescheM et al. (2012). Biodiversity effects on ecosystem functioning change along environmental stress gradients. Ecol Lett 15: 1397-1405.
88. Štursova M, Šnajdr J, Cajthaml T, Barta J, Šantruckova H, Baldrian P. (2014). When the forest dies: The response of forest soil fungi to a bark beetle-induced tree dieback. ISME J 8: 1920-1931.
89. Szava-Kovats RC, Zobel M, Pärtel M. (2011). The localregional species richness relationship: new perspectives on the null-hypothesis. Oikos 121: 321-326.
90. Tang CQ, Leasi F, Obertegger U, Kieneke A, Cbarraclough TG, Fontaneto D. (2012). The widely used small subunit 18 S rDNA molecule greatly underestimates true diversity in biodiversity surveys of the meiofauna. Proc Natl Acad Sci USA 109: 16208-16212.
91. Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I et al. (2015a). Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43.
92. Tedersoo L, Bahram M, Dickie IA. (2014a). Does host plant richness explain diversity of ectomycorrhizal fungi? Re-evaluation of Gao et al. (2013) data sets reveals sampling effects. Mol Ecol 23: 992-995.
93. Tedersoo L, Bahram M, Põlme S, Anslan S, Riit T, Kõljalg U et al. (2015b). Analytical biases in microbial diversity studies. Science (in press).
94. Tedersoo L, Bahram M, Põlme S, Kõljalg U, Yorou NS, Wijesundera R et al. (2014b). Global diversity and geography of soil fungi. Science 346: 1078.
95. Tedersoo L, Bahram M, Ryberg M, Otsing E, Kõljalg U, Abarenkov K. (2014c). Global biogeography of the ectomycorrhizal /sebacina lineage (Fungi, Sebacinales) as revealed from comparative phylogenetics analyses. Mol Ecol 23: 4168-4183.
96. Tedersoo L, Naadel T, Bahram M, Pritsch K, Buegger F, Leal M et al. (2012). Enzymatic activities and stable isotope patterns of ectomycorrhizal fungi in relation to phylogeny and exploration types in an afrotropical rain forest. New Phytol 195: 832-843.
97. Tedersoo L, Nilsson RH, Abarenkov K, Jairus T, Sadam A, Saar I et al. (2010). 454 Pyrosequencing and Sanger sequencing of tropical mycorrhizal fungi provide similar results but reveal substantial methodological biases. New Phytol 188: 291-301.
98. Tedersoo L, Smith ME. (2013). Lineages of ectomycorrhizal fungi revisited: foraging strategies and novel lineages revealed by sequences from belowground. Fungal Biol Rev 27: 83-99.
99. van der Heijden EW, Kuyper TW. (2003). Ecological strategies of ectomycorrhizal fungi of Salix repens: root manipulation versus root replacement. Oikos 103: 668-680.
100. van der Heijden MGA, Streitwolf-Engel R, Riedl R, Siegrist S, Neudecker A, Ineichen K et al. (2006). The mycorrhizal contribution to plant productivity, plant nutrition and soil structure in experimental grassland. New Phytol 172: 739-752.
101. van der Heijden MGA, Wiemken A, Sanders IR. (2003). Different arbuscular mycorrhizal fungi alter coexistence and resource distribution between cooccurring plant. New Phytol 157: 569-578.
102. Vehviläinen H, Koricheva J, Ruohomäki K. (2008). Effects of stand tree species composition and diversity on abundance of predaory arthropods. Oikos 117: 935-943.
103. Vetrovsky T, Baldrian P. (2013). The variability of the 16 S rRNA gene in bacterial genomes and its consequences for bacterial community analyses. PLoS One 8: e57923.
104. Wagg C, Bender SF, Widmer F, van der Heijden MGA. (2014). Soil biodiversity and soil community composition determine ecosystem multifunctionality. Proc Natl Acad Sci USA 111: 5266-5270.
105. Wagg C, Jansa J, Schmid B, van der Heijden MGA. (2011). Belowground biodiversity effects of plant symbionts support aboveground productivity. Ecol Lett 14: 1001-1009.
106. Wang X-C, Liu C, Huang L, Bengtsson-Palme J, Chen H, Zhang J-H et al. (2015). ITS1: A DNA barcode better than ITS2 in eukaryotes? Mol Ecol Res (in press).
107. Wardle DA, Bardgett RD, Klironomos JN, Setälä H, van der Putten WH, Wall DH. (2004). Ecological linkages between aboveground and belowground biota. Science 304: 1629-1633.
108. Wardle DA. (1999). Is 'sampling effect' a problem for experiments investigating biodiversity-ecosystem function relationships? Oikos 87: 403-407.
109. Wardle DA. (2002). Communities and Ecosystems: Linking the Aboveground and Belowground Components. Princeton University Press: New York, NY, USA.
110. White TJ, Bruns TD, Lee S, Taylor J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH (eds). PCR Protocols: A Guide to Methods and Applications. Academic Press: London, UK, pp 315-322.
111. Yao H, Song J, Liu C, Luo K, Han J, Li Y et al. (2010). Use of ITS2 region as the universal DNA barcode for plants and animals. PLoS One 5: e13102.
112. Zhan A, Bailey SA, Heath DJ, MacIsaac HJ. (2014). Performance comparison of genetic markers for high-throughput sequencing-based biodiversity assessment in complex communities. Mol Ecol Res 14: 1049-1059.