Lupeol inhibits LPS-induced NF-kappa B signaling in intestinal epithelial cells and macrophages, and attenuates acute and chronic murine colitis

Life Sciences

Volumn 146, Issue , 2016, Pages 100-108

  Lee, Changhyun ^a,b   Lee, Jung Won ^a   Seo, Ji Yeon ^a,b   Hwang, Sung Wook ^a   Im, Jong Pil ^a   Kim, Joo Sung ^a,b  

^a Seoul National University   (South Korea)

^b Seoul National University Hospital Healthcare System Gangnam Center   (South Korea)

Author keywords

Inflammatory bowel diseases; Lupeol; Mice; NF kappa B; Pentacyclic triterpenes

Indexed keywords

ANTIINFLAMMATORY AGENT; DEXTRAN SULFATE; I KAPPA B ALPHA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 10; INTERLEUKIN 12; INTERLEUKIN 6; INTERLEUKIN 8; LIPOPOLYSACCHARIDE; LUPEOL; MESALAZINE; TUMOR NECROSIS FACTOR ALPHA; CYTOKINE; IL10 PROTEIN, MOUSE; NONSTEROID ANTIINFLAMMATORY AGENT; PENTACYCLIC TRITERPENE;

ACUTE DISEASE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIINFLAMMATORY ACTIVITY; ARTICLE; CELL LINE RAW 264.7; CHRONIC DISEASE; COLITIS; COLON MUCOSA; CONTROLLED STUDY; CYTOKINE PRODUCTION; DISEASE ACTIVITY SCORE; DNA BINDING; DRUG EFFECT; DRUG MECHANISM; DRUG POTENCY; DRUG SCREENING; ENZYME LINKED IMMUNOSORBENT ASSAY; GEL MOBILITY SHIFT ASSAY; HISTOPATHOLOGY; HUMAN; HUMAN CELL; IN VIVO STUDY; INTESTINE EPITHELIUM CELL; MACROPHAGE; MOUSE; NONHUMAN; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; WESTERN BLOTTING; ANIMAL; ANTAGONISTS AND INHIBITORS; BIOSYNTHESIS; C57BL MOUSE; CELLS; DRUG EFFECTS; EPITHELIUM CELL; GENETICS; INTESTINE MUCOSA; KNOCKOUT MOUSE; METABOLISM; PATHOLOGY; RAW 264.7 CELL LINE;

ANIMALS; ANTI-INFLAMMATORY AGENTS, NON-STEROIDAL; CELLS; COLITIS; CYTOKINES; EPITHELIAL CELLS; HUMANS; INTERLEUKIN-10; INTESTINAL MUCOSA; LIPOPOLYSACCHARIDES; MACROPHAGES; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; NF-KAPPA B; PENTACYCLIC TRITERPENES; RAW 264.7 CELLS; SIGNAL TRANSDUCTION;

EID: 84954213956     PISSN: 00243205     EISSN: 18790631     Source Type: Journal    
DOI: 10.1016/j.lfs.2016.01.001     Document Type: Article

References (33)

1. I. Atreya, R. Atreya, and M.F. Neurath NF-kappaB in inflammatory bowel disease J. Intern. Med. 263 2008 591 596
2. S. Bani, A. Kaul, B. Khan, S.F. Ahmad, K.A. Suri, B.D. Gupta, and et al. Suppression of T lymphocyte activity by lupeol isolated from Crataeva religiosa Phytother. Res. 20 2006 279 287
3. D.J. Berg, J. Zhang, J.V. Weinstock, H.F. Ismail, K.A. Earle, H. Alila, and et al. Rapid development of colitis in NSAID-treated IL-10-deficient mice Gastroenterology 123 2002 1527 1542
4. J. Chun, C. Lee, S.W. Hwang, J.P. Im, and J.S. Kim Ursolic acid inhibits nuclear factor-kappaB signaling in intestinal epithelial cells and macrophages, and attenuates experimental colitis in mice Life Sci. 110 2014 23 34
5. M.L. Clapper, M.A. Gary, R.A. Coudry, S. Litwin, W.C. Chang, K. Devarajan, and et al. 5-Aminosalicylic acid inhibits colitis-associated colorectal dysplasias in the mouse model of azoxymethane/dextran sulfate sodium-induced colitis Inflamm. Bowel Dis. 14 2008 1341 1347
6. M. Dey, P. Kuhn, D. Ribnicky, V. Premkumar, K. Reuhl, and I. Raskin Dietary phenethylisothiocyanate attenuates bowel inflammation in mice BMC Chem. Biol. 10 2010 4
7. L.A. Dieleman, B.U. Ridwan, G.S. Tennyson, K.W. Beagley, R.P. Bucy, and C.O. Elson Dextran sulfate sodium-induced colitis occurs in severe combined immunodeficient mice Gastroenterology 107 1994 1643 1652
8. S. Fiorucci, S. Orlandi, A. Mencarelli, G. Caliendo, V. Santagada, E. Distrutti, and et al. Enhanced activity of a hydrogen sulphide-releasing derivative of mesalamine (ATB-429) in a mouse model of colitis Br. J. Pharmacol. 150 2007 996 1002
9. D. Gilardi, G. Fiorino, M. Genua, M. Allocca, and S. Danese Complementary and alternative medicine in inflammatory bowel diseases: what is the future in the field of herbal medicine? Expert Rev. Gastroenterol. Hepatol. 8 2014 835 846
10. Y. Ikeda, A. Murakami, and H. Ohigashi Ursolic acid: an anti- and pro-inflammatory triterpenoid Mol. Nutr. Food Res. 52 2008 26 42
11. C. Jobin, S. Haskill, L. Mayer, A. Panja, and R.B. Sartor Evidence for altered regulation of I kappa B alpha degradation in human colonic epithelial cells J. Immunol. 158 1997 226 234 (Baltimore, MD: 1950)
12. J.S. Kim, and C. Jobin The flavonoid luteolin prevents lipopolysaccharide-induced NF-kappaB signalling and gene expression by blocking IkappaB kinase activity in intestinal epithelial cells and bone-marrow derived dendritic cells Immunology 115 2005 375 387
13. S.J. Koh, J.M. Kim, I.K. Kim, S.H. Ko, and J.S. Kim Anti-inflammatory mechanism of metformin and its effects in intestinal inflammation and colitis-associated colon cancer J. Gastroenterol. Hepatol. 29 2014 502 510
14. C.F. Krieglstein, W.H. Cerwinka, A.G. Sprague, F.S. Laroux, M.B. Grisham, V.E. Koteliansky, and et al. Collagen-binding integrin alpha1beta1 regulates intestinal inflammation in experimental colitis J. Clin. Invest. 110 2002 1773 1782
15. A. Kumari, and P. Kakkar Lupeol prevents acetaminophen-induced in vivo hepatotoxicity by altering the Bax/Bcl-2 and oxidative stress-mediated mitochondrial signaling cascade Life Sci. 90 2012 561 570
16. C. Lee, J. Chun, S.W. Hwang, S.J. Kang, J.P. Im, and J.S. Kim The effect of intestinal alkaline phosphatase on intestinal epithelial cells, macrophages and chronic colitis in mice Life Sci. 100 2014 118 124
17. C. Lee, J. Chun, S.W. Hwang, S.J. Kang, J.P. Im, and J.S. Kim Enalapril inhibits nuclear factor-kappaB signaling in intestinal epithelial cells and peritoneal macrophages and attenuates experimental colitis in mice Life Sci. 95 2014 29 39
18. T.K. Lee, A. Castilho, V.C. Cheung, K.H. Tang, S. Ma, and I.O. Ng Lupeol targets liver tumor-initiating cells through phosphatase and tensin homolog modulation Hepatology 53 2011 160 170 (Baltimore, MD)
19. L. Li, L. Wang, Z. Wu, L. Yao, Y. Wu, L. Huang, and et al. Anthocyanin-rich fractions from red raspberries attenuate inflammation in both RAW264.7 macrophages and a mouse model of colitis Sci. Rep. 4 2014
20. K.J. Maloy, and F. Powrie Intestinal homeostasis and its breakdown in inflammatory bowel disease Nature 474 2011 298 306
21. J.A. Marrero, K.A. Matkowskyj, K. Yung, G. Hecht, and R.V. Benya Dextran sulfate sodium-induced murine colitis activates NF-kappaB and increases galanin-1 receptor expression Am. J. Physiol. Gastrointest. Liver Physiol. 278 2000 G797 G804
22. M.F. Neurath, S. Pettersson, K.H. Meyerzum Buschenfelde, and W. Strober Local administration of antisense phosphorothioate oligonucleotides to the p65 subunit of NF-kappa B abrogates established experimental colitis in mice Nat. Med. 2 1996 998 1004
23. L.W. Peterson, and D. Artis Intestinal epithelial cells: regulators of barrier function and immune homeostasis Nat. Rev. Immunol. 14 2014 141 153
24. P. Renard, I. Ernest, A. Houbion, M. Art, H. Le Calvez, M. Raes, and et al. Development of a sensitive multi-well colorimetric assay for active NFkappaB Nucleic Acids Res. 29 2001 E21
25. M. Saleem Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene Cancer Lett. 285 2009 109 115
26. M. Saleem, F. Afaq, V.M. Adhami, and H. Mukhtar Lupeol modulates NF-kappaB and PI3K/Akt pathways and inhibits skin cancer in CD-1 mice Oncogene 23 2004 5203 5214
27. V. Saratha, and S.P. Subramanian Lupeol, a triterpenoid isolated from Calotropis gigantea latex ameliorates the primary and secondary complications of FCA induced adjuvant disease in experimental rats Inflammopharmacology 20 2012 27 37
28. H.R. Siddique, and M. Saleem Beneficial health effects of lupeol triterpene: a review of preclinical studies Life Sci. 88 2011 285 293
29. I. Singh, I. Bishnoi, V. Agarwal, and S. Bhatt Prospective randomized clinical trial comparing phytotherapy with potassium citrate in management of minimal burden (
30. K.S. Siveen, A.H. Nguyen, J.H. Lee, F. Li, S.S. Singh, A.P. Kumar, and et al. Negative regulation of signal transducer and activator of transcription-3 signalling cascade by lupeol inhibits growth and induces apoptosis in hepatocellular carcinoma cells Br. J. Cancer 111 2014 1327 1337
31. P.D. Smith, L.E. Smythies, R. Shen, T. Greenwell-Wild, M. Gliozzi, and S.M. Wahl Intestinal macrophages and response to microbial encroachment Mucosal Immunol. 4 2011 31 42
32. X.H. Wang, S.Y. Zhou, Z.Z. Qian, H.L. Zhang, L.H. Qiu, Z. Song, and et al. Evaluation of toxicity and single-dose pharmacokinetics of intravenous ursolic acid liposomes in healthy adult volunteers and patients with advanced solid tumors Expert Opin. Drug Metab. Toxicol. 9 2013 117 125
33. H.J. You, C.Y. Choi, J.Y. Kim, S.J. Park, K.S. Hahm, and H.G. Jeong Ursolic acid enhances nitric oxide and tumor necrosis factor-alpha production via nuclear factor-kappaB activation in the resting macrophages FEBS Lett. 509 2001 156 160