Subtle structural changes in the Asp251Gly/Gln307His P450 BM3 mutant responsible for new activity toward diclofenac, tolbutamide and ibuprofen

Archives of Biochemistry and Biophysics

Volumn 602, Issue , 2016, Pages 106-115

  Di Nardo, Giovanna ^a   Dell'Angelo, Valentina ^a   Catucci, Gianluca ^a   Sadeghi, Sheila J ^a   Gilardi, Gianfranco ^a  

^a UNIVERSITY OF TURIN   (Italy)

Author keywords

Biocatalysis; Conformational equilibrium; Cytochrome P450; Protein engineering

Indexed keywords

2 HYDROXYIBUPROFEN; 4 HYDROXYTOLBUTAMIDE; 4' HYDROXYDICLOFENAC; CYTOCHROME P450 BM3; CYTOCHROME P450 BM3 A2; DICLOFENAC DERIVATIVE; DRUG METABOLITE; IBUPROFEN DERIVATIVE; TOLBUTAMIDE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CYTOCHROME P450; DICLOFENAC; FLAVOCYTOCHROME P450 BM3 MONOXYGENASES; IBUPROFEN; PROTEIN BINDING; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; CRYSTALLIZATION; DISSOCIATION CONSTANT; DRUG BINDING; ELECTRON TRANSPORT; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME CONFORMATION; ENZYME STRUCTURE; GENE MUTATION; HYDROGEN BOND; IONIC STRENGTH; MELTING POINT; MOLECULAR DOCKING; MUTAGENESIS; OXIDATION REDUCTION STATE; PH; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; STEADY STATE; SUBSTRATE CONCENTRATION; THERMOSTABILITY; AMINO ACID SEQUENCE; BINDING SITE; CHEMISTRY; COMPARATIVE STUDY; COMPUTER SIMULATION; ENZYME ACTIVATION; GENETICS; MOLECULAR GENETICS; MUTATION; PROCEDURES; PROTEIN CONFORMATION; STRUCTURE ACTIVITY RELATION; ULTRASTRUCTURE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; COMPUTER SIMULATION; CYTOCHROME P-450 ENZYME SYSTEM; DICLOFENAC; ENZYME ACTIVATION; IBUPROFEN; MOLECULAR DOCKING SIMULATION; MOLECULAR SEQUENCE DATA; MUTATION; NADPH-FERRIHEMOPROTEIN REDUCTASE; PROTEIN BINDING; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP; TOLBUTAMIDE;

EID: 84951949905     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2015.12.005     Document Type: Article

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