Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris: Optimizing Yield and N-terminal Processing

Molecular Biotechnology

Volumn 57, Issue 11-12, 2015, Pages 1010-1017

  Tanghe, Magali ^a   Danneels, Barbara ^a   Camattari, Andrea ^b,c   Glieder, Anton ^c   Vandenberghe, Isabel ^a   Devreese, Bart ^a   Stals, Ingeborg ^a   Desmet, Tom ^a  

^a GHENT UNIVERSITY   (Belgium)

^b AGENCY FOR SCIENCE   (Singapore)

^c GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Auxiliary activity family 9; Cellulose hydrolysis; Lytic polysaccharide monooxygenase; N terminal processing; Pichia pastoris; Trichoderma reesei Cel61A (TrCel61A)

Indexed keywords

AMINO ACIDS; CELLULOSE; ENZYME ACTIVITY; ENZYMES; PROTEINS; RECOMBINANT PROTEINS;

AUXILIARY ACTIVITY FAMILY 9; CELLULOSE HYDROLYSIS; MONOOXYGENASES; N-TERMINALS; PICHIA PASTORIS; TRICHODERMA REESEI;

YEAST;

FUNGAL PROTEIN; TRICHODERMA REESEI CEL61A PROTEIN; UNCLASSIFIED DRUG; CELLULOSE; FUNGAL DNA; MATING FACTOR; MIXED FUNCTION OXIDASE; PEPTIDE; POLYSACCHARIDE;

ARTICLE; DEGRADATION; FERMENTATION; KOMAGATAELLA PASTORIS; N TERMINAL PROCESSING; NONHUMAN; PROCESSING; PROTEIN EXPRESSION; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE; AMINO ACID SEQUENCE; BIOMASS; BIOSYNTHESIS; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENETICS; HYDROLYSIS; METABOLISM; MOLECULAR GENETICS; PICHIA; TRICHODERMA;

AMINO ACID SEQUENCE; BIOMASS; CELLULOSE; DNA, FUNGAL; FERMENTATION; FUNGAL PROTEINS; HYDROLYSIS; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; PEPTIDES; PICHIA; POLYSACCHARIDES; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; TRICHODERMA;

EID: 84945469678     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-015-9887-9     Document Type: Article

References (41)

1. Vu, V. V., Beeson, W. T., Span, E. A., et al. (2014). A family of starch-active polysaccharide monooxygenases. Proc Natl Acad Sci USA,111, 13822–13827.
2. Levasseur, A., Drula, E., Lombard, V., et al. (2013). Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes. Biotechnol Biofuels,6, 41.
3. Lo Leggio, L., Simmons, T. J., Poulsen, J.-C. N., et al. (2015). Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase. Nat Commun,6, 5961.
4. Horn, S. J., Vaaje-Kolstad, G., Westereng, B., et al. (2012). Novel enzymes for the degradation of cellulose. Biotechnol Biofuels,5, 45.
5. Dimarogona, M., Topakas, E., Olsson, L., et al. (2012). Lignin boosts the cellulase performance of a GH-61 enzyme from Sporotrichum thermophile. Bioresour Technol,110, 480–487.
6. Beeson, W. T., Phillips, C. M., Cate, J. H. D., et al. (2012). Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases. J Am Chem Soc,134, 890–892.
7. Li, X., Beeson, W. T., Phillips, C. M., et al. (2012). Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases. Structure,20, 1051–1061.
8. Ekwe, E., Morgenstern, I., Tsang, A., et al. (2013). Non-hydrolytic cellulose active proteins: research progress and potential application in biorefineries. Ind Biotechnol,9, 123–131.
9. Dimarogona, M., Topakas, E., & Christakopoulos, P. (2013). Recalcitrant polysaccharide degradation by novel oxidative biocatalysts. Appl Microbiol Biotechnol,97, 8455–8465.
10. Karkehabadi, S., Hansson, H., Kim, S., et al. (2008). The first structure of a glycoside hydrolase family 61 member, Cel61B from Hypocrea jecorina, at 1.6 A resolution. J Mol Biol,383, 144–154.
11. Westereng, B., Ishida, T., Vaaje-Kolstad, G., et al. (2011). The putative endoglucanase PcGH61D from Phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose. PLoS ONE,6, e27807.
12. Kittl, R., Kracher, D., Burgstaller, D., et al. (2012). Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay. Biotechnol Biofuels,5, 79.
13. Bey, M., Zhou, S., Poidevin, L., et al. (2013). Cello-oligosaccharide oxidation reveals differences between two lytic polysaccharide monooxygenases (family GH61) from Podospora anserina. Appl Environ Microbiol,79, 488–496.
14. Daly, R., & Hearn, M. T. W. (2005). Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J Mol Recognit,18, 119–138.
15. Quinlan, R. J., Sweeney, M. D., Lo Leggio, L., et al. (2011). Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components. Proc Natl Acad Sci USA,108, 15079–15084.
16. Hegde, R. S., & Bernstein, H. D. (2006). The surprising complexity of signal sequences. Trends Biochem Sci,31, 563–571.
17. Merino, S. T., Cherry, J., & Ave, D. (2007). Progress and challenges in enzyme development for biomass utilization. Adv Biochem Eng Biotechnol,108, 95–120.
18. Wilson, D. B. (2009). Cellulases and biofuels. Curr Opin Biotechnol,20, 295–299.
19. Kubicek, C. P., Mikus, M., Schuster, A., et al. (2009). Metabolic engineering strategies for the improvement of cellulase production by Hypocrea jecorina. Biotechnol Biofuels,2, 19.
20. Saloheimo, M., Nakari-Setälä, T., Tenkanen, M., et al. (1997). cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast. Eur J Biochem,249, 584–591.
21. Karlsson, J., Saloheimo, M., Siika-Aho, M., et al. (2001). Homologous expression and characterization of Cel61A (EG IV) of Trichoderma reesei. Eur J Biochem,268, 6498–6507.
22. Näätsaari, L., Mistlberger, B., Ruth, C., et al. (2012). Deletion of the pichia pastoris ku70 homologue facilitates platform strain generation for gene expression and synthetic biology. PLoS ONE,7, e39720.
23. Sanchis, J., Fernández, L., Carballeira, J. D., et al. (2008). Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates. Appl Microbiol Biotechnol,81, 387–397.
24. Gibson, D. G., Young, L., Chuang, R., et al. (2009). Enzymatic assembly of DNA molecules up to several hundred kilobases.,6, 12–16.
25. Lin-Cereghino, J., Wong, W. W., Xiong, S., et al. (2005). Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris. Biotechniques,38, 44–48.
26. Weis, R., Luiten, R., Skranc, W., et al. (2004). Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena. FEMS Yeast Res,5, 179–189.
27. De Winter, K., Šimčíková, D., Schalck, B., et al. (2013). Chemoenzymatic synthesis of α-l-rhamnosides using recombinant α-l-rhamnosidase from Aspergillus terreus. Bioresour Technol,147, 640–644.
28. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature,227, 680–685.
29. Sun, L. W., Zhao, Y., Niu, L. P., et al. (2011). A rapid method for determining the concentration of recombinant protein secreted from Pichia pastoris. J Phys: Conf Ser,276, 012144.
30. Wood, T. M. (1988). Preparation of crystalline, amorphous, and dyed cellulase substrates. Methods Enzymol,160, 19–25.
31. Forsberg, Z., Vaaje-Kolstad, G., Westereng, B., et al. (2011). Cleavage of cellulose by a CBM33 protein. Protein Sci,20, 1479–1483.
32. Cregg, J. M., Cereghino, J. L., Shi, J., et al. (2000). Recombinant protein expression in Pichia pastoris. Mol Biotechnol,16, 23–52.
33. Hohenblum, H., Gasser, B., Maurer, M., et al. (2004). Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol Bioeng,85, 367–375.
34. Mellitzer, A., Weis, R., Glieder, A., et al. (2012). Expression of lignocellulolytic enzymes in Pichia pastoris. Microb Cell Fact,11, 61.
35. Sygmund, C., Kracher, D., Scheiblbrandner, S., et al. (2012). Characterization of the two Neurospora crassa cellobiose dehydrogenases and their connection to oxidative cellulose degradation. Appl Environ Microbiol,78, 6161–6171.
36. Cereghino, J. L., & Cregg, J. M. (2000). Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev,24, 45–66.
37. Govindappa, N., Hanumanthappa, M., Venkatarangaiah, K., et al. (2014). A new signal sequence for recombinant protein secretion in Pichia pastoris. J Microbiol Biotechnol,24, 337–345.
38. Isaksen, T., Westereng, B., Aachmann, F. L., et al. (2014). A C4-oxidizing lytic polysaccharide monooxygenase cleaving both cellulose and cello-oligosaccharides. J Biol Chem,289, 2632–2642.
39. Vu, V. V., Beeson, W. T., Phillips, C. M., et al. (2014). Determinants of regioselective hydroxylation in the fungal polysaccharide monooxygenases. J Am Chem Soc,136, 562–565.
40. Forsberg, Z., Mackenzie, A. K., Sorlie, M., et al. (2014). Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases. Proc Natl Acad Sci,111, 8446–8451.
41. Ahmad, M., Hirz, M., Pichler, H., et al. (2014). Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production. Appl Microbiol Biotechnol,98, 5301–5317.