Characterization of the two Neurospora crassa cellobiose dehydrogenases and their connection to oxidative cellulose degradation

Applied and Environmental Microbiology

Volumn 78, Issue 17, 2012, Pages 6161-6171

  Sygmund, Christoph ^a   Kracher, Daniel ^a   Scheiblbrandner, Stefan ^a   Zahma, Kawah ^a   Felice, Alfons K G ^a   Harreither, Wolfgang ^a   Kittl, Roman ^a   Ludwig, Roland ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDIC PH; CARBOHYDRATE-BINDING MODULES; CATALYTIC CONSTANTS; CATALYTIC EFFICIENCIES; CELLOBIOSE DEHYDROGENASE; CELLULOSE DEGRADATION; CELLULOSE HYDROLYSIS; CELLULOSIC MATERIAL; COFACTORS; CYTOCHROME C; ELECTRON TRANSFER; ENZYME SYSTEMS; GLYCOSIDE HYDROLASES; IN-VIVO; MONOOXYGENASES; NEUROSPORA CRASSA; P. PASTORIS; PH-DEPENDENT; PICHIA PASTORIS; REDOX POTENTIALS; SECRETOME; SEQUENCE IDENTITY;

CARBOHYDRATES; DEGRADATION; PORPHYRINS; REDOX REACTIONS;

CELLULOSE;

CELLOBIOSE QUINONE OXIDOREDUCTASE; CELLOBIOSE-QUINONE OXIDOREDUCTASE; CELLULOSE; OXIDOREDUCTASE; RECOMBINANT PROTEIN;

CARBOHYDRATE; CATALYSIS; CELLULOSE; CYTOCHROME; DEGRADATION; ELECTRON; ENZYME ACTIVITY; FUNGUS; GENOME; HYDROLYSIS; OXIDATION; PH;

ARTICLE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; NEUROSPORA CRASSA; OXIDATION REDUCTION REACTION; PH; PICHIA;

CARBOHYDRATE DEHYDROGENASES; CELLULOSE; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; NEUROSPORA CRASSA; OXIDATION-REDUCTION; PICHIA; RECOMBINANT PROTEINS;

NEUROSPORA CRASSA; PICHIA PASTORIS;

EID: 84866154418     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01503-12     Document Type: Article

References (43)

1. Beeson WT, Phillips CM, Cate JHD, Marletta MA. 2012. Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases. J. Am. Chem. Soc. 134:890-892.
2. Cameron MD, Aust SD. 2001. Cellobiose dehydrogenase-an extracellular fungal flavocytochrome. Enzyme Microb. Technol. 28:129-138.
3. Coman V, Harreither W, Ludwig R, Haltrich D, Gorton L. 2007. Investigation of electron transfer between cellobiose dehydrogenase from Myriococcum thermophilum and gold electrodes. Chem. Analityczna 52:945-960.
4. Eberhart BM, Beck RS, Goolsby KM. 1977. Cellulase of Neurospora crassa. J. Bacteriol. 130:181-186.
5. Fan Z, et al. 2012. A novel biochemical route for fuels and chemicals production from cellulosic biomass. PLoS One 7:e31693. doi:10.1371/ journal.pone.0031693.
6. Haladjian J, Bianco P, Nunzi F, Bruschi M. 1994. A permselectivemembrane electrode for the electrochemical study of redox proteins. Application to cytochrome C552 from Thiobacillus ferrooxidans. Anal. Chim. Acta 289:15-20.
7. Harreither W, Coman V, Ludwig R, Haltrich D, Gorton L. 2007. Investigation of graphite electrodes modified with cellobiose dehydrogenase from the ascomycete Myriococcum thermophilum. Electroanalysis 19:172-180.
8. Harreither W, et al. 2011. Catalytic properties and classification of cellobiose dehydrogenases from ascomycetes. Appl. Environ. Microbiol. 77:1804-1815.
9. Harreither W, et al. 2009. Cellobiose dehydrogenase from the ligninolytic basidiomycete Ceriporiopsis subvermispora. Appl. Environ. Microbiol. 75:2750-2757.
10. Harris PV, et al. 2010. Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family. Biochemistry 49:3305-3316.
11. Henriksson G, Johansson G, Pettersson G. 2000. A critical review of cellobiose dehydrogenases. J. Biotechnol. 78:93-113.
12. Henriksson G, Sild V, Szabo IJ, Pettersson G, Johansson G. 1998. Substrate specificity of cellobiose dehydrogenase from Phanerochaete chrysosporium. Biochim. Biophys. Acta 1383:48-54.
13. Igarashi K, et al. 1999. Cellobiose dehydrogenase from the fungi Phanerochaete chrysosporium and Humicola insolen. A flavohemoprotein from Humicola insolens contains 6-hydroxy-fad as the dominant active cofactor. J. Biol. Chem. 274:3338-3344.
14. Igarashi K, et al. 2005. Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium. FEBS J. 272:2869-2877.
15. Karapetyan KN, et al. 2006. Properties of neutral cellobiose dehydrogenase from the ascomycete Chaetomium s. INBI 2-26(-) and comparison with basidiomycetous cellobiose dehydrogenases. J. Biotechnol. 121:34-48.
16. Karkehabadi S, et al. 2008. The first structure of a glycoside hydrolase family 61 member, Cel61B from Hypocrea jecorina, at 1.6 Å resolution. J. Mol. Biol. 383:144-154.
17. Langston JA, et al. 2011. Oxidoreductive cellulose depolymerization by the enzymes cellobiose dehydrogenase and glycoside hydrolase 61. Appl. Environ. Microbiol. 77:7007-7015.
18. Letourneur O, et al. 2001. Characterization of Toxoplasma gondii surface antigen I (SAGI) secreted from Pichia pastoris: evidence of hyper O-glycosylation. Biotechnol. Appl. Biochem. 33:35-45.
19. Ludwig R, Haltrich D. 2002. Cellobiose dehydrogenase production by Sclerotium species pathogenic to plants. Lett. Appl. Microbiol. 35:261-266.
20. Ludwig R, et al. 2004. Characterisation of cellobiose dehydrogenases from the white-rot fungi Trametes pubescens and Trametes villosa. Appl. Microbiol. Biotechnol. 64:213-222.
21. McIlvaine TC. 1921. A buffer solution for colorimetric comparison. J. Biol. Chem. 49:183-186.
22. Phillips CM, Beeson WT, Cate JH, Marletta MA. 2011. Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by Neurospora crassa. ACS Chem. Biol. 6:1399-1406.
23. Phillips CM, Iavarone AT, Marletta MA. 2011. Quantitative proteomic approach for cellulose degradation by Neurospora crassa. J. Proteome Res. 10:4177-4185.
24. Quinlan RJ, et al. 2011. Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components. Proc. Natl. Acad. Sci. U. S. A. 108:15079-15084.
25. Rao M, Mishra C, Keskar S, Srinivasan MC. 1985. Production of ethanol from wood and agricultural residues by Neurospora crassa. Enzyme Microb. Technol. 7:625-628.
26. Rogers MS, Jones GD, Antonini G, Wilson MT, Brunori M. 1994. Electron transfer from Phanerochaete chrysosporium cellobiose oxidase to equine cytochrome c and Pseudomonas aeruginosa cytochrome c-551. Biochem. J. 298:329-334.
27. Sun J, Glass NL. 2011. Identification of the CRE-1 cellulolytic regulon in Neurospora crassa. PLoS One 6:e25654. doi:10.1371/journal.pone.0025654.
28. Sun J, Tian C, Diamond S, Glass NL. 2012. Deciphering transcriptional regulatory mechanisms associated with hemicellulose degradation in Neurospora crassa. Eukaryot. Cell 11:482-493.
29. Sygmund C, et al. 2012. Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris. Appl. Microbiol. Biotechnol. 94:695-704.
30. Sygmund C, et al. 2011. Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris. Microb. Cell Fact. 10:106.
31. Tasca F, et al. 2011. A third generation glucose biosensor based on cellobiose dehydrogenase from Corynascus thermophilus and single-walled carbon nanotubes. Analyst 136:2033-2036.
32. Tian C, et al. 2009. Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 106:22157-22162.
33. Trimble RB, et al. 2004. Characterization of N- and O-linked glycosylation of recombinant human bile salt-stimulated lipase secreted by Pichia pastoris. Glycobiology 14:265-274.
34. Tuomela M, Vikman M, Hatakka A, Itävaara M. 2000. Biodegradation of lignin in a compost environment: a review. Bioresour. Technol. 72:169-183.
35. Vaaje-Kolstad G, Horn SJ, Van Aalten DMF, Synstad B, Eijsink VGH. 2005. The non-catalytic chitin-binding protein CBP21 from Serratia marcescens is essential for chitin degradation. J. Biol. Chem. 280:28492-28497.
36. Vaaje-Kolstad G, Houston DR, Riemen AHK, Eijsink VGH, Van Aalten DMF. 2005. Crystal structure and binding properties of the Serratia marcescens chitin-binding protein CBP21. J. Biol. Chem. 280:11313-11319.
37. Vaaje-Kolstad G, et al. 2010. An oxidative enzyme boosting the enzymatic conversion of recalcitrant polysaccharides. Science 330:219-222.
38. Weis R, et al. 2004. Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena. FEMS Yeast Res. 5:179-189.
39. Westereng B, et al. 2011. The putative endoglucanase PcGH61D from Phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose. PLoS One 6:e27807. doi:10.1371/journal.pone.0027807.
40. Yazdi MT, Woodward JR, Radford A. 1990. The cellulase complex of Neurospora crassa: activity, stability and release. J. Gen. Microbiol. 136:1313-1319.
41. Zámocký M, Hallberg M, Ludwig R, Divne C, Haltrich D. 2004. Ancestral gene fusion in cellobiose dehydrogenases reflects a specific evolution of GMC oxidoreductases in fungi. Gene 338:1-14.
42. Zámocký M, et al. 2006. Cellobiose dehydrogenase-a flavocytochrome from wood-degrading, phytopathogenic and saprotropic fungi. Curr. Protein Pept. Sci. 7:255-280.
43. Zhang R, Fan Z, Kasuga T. 2011. Expression of cellobiose dehydrogenase from Neurospora crassa in Pichia pastoris and its purification and characterization. Protein Expr. Purif. 75:63-69.