cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast

European Journal of Biochemistry

Volumn 249, Issue 2, 1997, Pages 584-591

  Saloheimo, Markku ^a   Nakari SETÄLÄ, Tiina ^a   Tenkanen, Maija ^a   Penttilä, Merja ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

Author keywords

Cellulase; Endoglucanase; Regulation; Trichoderma; Yeast

Indexed keywords

CELLULASE; GLUCAN SYNTHASE;

ARTICLE; ENZYME ACTIVITY; GENE EXPRESSION; MOLECULAR CLONING; PRIORITY JOURNAL; TRICHODERMA REESEI; YEAST;

AGARICUS BISPORUS; ESCHERICHIA COLI; FUNGI; HYPOCREA JECORINA; TRICHODERMA;

EID: 0030669760     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00584.x     Document Type: Article

References (44)

1. Aalto, M. K., Ronne, H. & Keränen, S. (1993) Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport, EMBO J. 12, 4095-4104.
2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403-410.
3. Armesilla, A. L., Thurston, C. F. & Yague, E. (1994) CEL1: a novel cellulose binding protein secreted by Agaricus bisporus during growth on crystalline cellulose, FEMS Microbiol. Lett. 116, 293-300.
4. Bernfeld, P. (1955) Amylases, α and β, Methods Enzymol. 1, 149-158.
5. Chen, C. M. Gritzali, M. & Stafford, D. W. (1987) Nucleotide sequence and deduced primary structure of cellobiohydrolase II from Trichoderma reesei. Biotechnology 5, 274 -278.
6. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease, Biochem. J. 18, 5294-5299.
7. Divne, C., Ståhlberg, J., Reinikainen, T., Ruohonen, L., Pettersson, G., Knowles, J. K. C., Teeri, T. T. & Jones, A. (1994) The three-dimensional crystal structure of the catalytic core cellobiohydrolase I from Trichoderma reesei, Science 265, 524-528.
8. Ducros, V., Czjzek, M., Belaich, A., Gaudin, C., Fieribe, H.-P., Belaich, J.-P., Davies, G. & Haser, R. (1995) Crystal structure of the catalytic domain of a bacterial cellulase belonging to family 5, Structure 3, 939-949.
9. Fägerstam, L. G., Pettersson, L. G. & Engström, J. Å. (1984) The primary structure of a 1,4-β-glucan cellobiohydrolase from the fungus Trichoderma reesei QM9414, FEBS Lett. 167, 309-315.
10. Gaboriaud, C., Bissery, V., Benchetrit, T. & Mornon, J. P. (1987) Hydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences, FEBS Lett. 224, 149-155.
11. Gietz, D., St Jean, A., Woods, R. A. & Schiestl, R. H. (1992) Improved method for high efficiency transformation of intact yeast cells, Nucleic Acids Res. 20, 1425.
12. Henrissat, B. & Bairoch, A. (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities, Biochem. J. 293, 781-788.
13. Hoffrén, A.-M., Teeri, T. T. & Teleman, O. (1995) Molecular dynamics simulation of fungal cellulose-binding domains: differences in molecular rigidity but a preserved cellulose binding surface. Protein Eng. 8, 443-450.
14. Ilmén, M., Saloheimo, A., Onnela, M.-L. & Penttilä, M. E. (1997) Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei, Appl. Environ. Microbiol. 63, 1298-1306.
15. Kraulis, P. J., Glore, G. M., Nilges, M., Jones, T. A., Pettersson, G., Knowles, J. & Gronenborn, A. M. (1989) Determination of the three-dimensional structure of the C-terminal domain of the cellobiohydrolase I from Trichoderma reesei. A study using nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing, Biochemistry 28, 7241-7257.
16. Laemmli, U. K. (1970) Cleavage of structural ptoteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
17. Lemesle-Varloot, L., Henrissat, B., Gaboriaud, C., Bissery, V., Morgat, A. & Mornon, J.-P. (1990) Hydrophobic cluster analysis: procedures to derive structural and functional information from 2D- representation of protein sequences, Biochimie (Paris) 21, 555-574.
18. Mandels, M., Weber, J. & Parizek, R. (1971) Enhanced cellulase production by a mutant of Trichoderma viridae, Appl. Microbiol. 21, 152-154.
19. Margolles-Clark, E., Tenkanen, M., Nakari-Setälä, T. & Penttilä, M. (1996a) Cloning of genes encoding α-L-arabinofuranosidase and β-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae, Appl. Environ. Microbiol. 62, 3840-3846.
20. Margolles-Clark, E., Tenkanen, M., Söderlund, H. & Penttilä, M. (1996b) Acetyl xylan esterase from Trichoderma reesei contains an active site serine and a cellulose-binding domain, Eur. J. Biochem. 237, 553-560.
21. Nakari-Setälä, T. & Penttilä, M. (1995) Production of Trichoderma reesei cellulases on glucose-containing media, Appl. Env. Microbiol. 61, 3650-3655.
22. Nevalainen, H. & Penttilä, M. (1995) Molecular biology of cellulolytic fungi, in The Mycota II, Genetics and biotechnology (Kuck, U., ed.) pp. 303-319, Springer-Verlag, Berlin.
23. Penttilä, M., Lehtovaara, P., Nevalainen, H., Bhikhabhai, R. & Knowles, J. (1986) Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene, Gene (Amst.) 45, 253-263.
24. Penttilä, M., Nevalainen, H., Rättö, M., Salminen, E. & Knowles, J. (1987a) A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei, Gene (Amst.) 61, 155-164.
25. Penttilä, M., André, L., Saloheimo, M., Lehtovaara, P. & Knowles, J. (1987b) Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae, Yeast 3, 175-185.
26. Penttilä, M. E., André, L., Lehtovaara, P., Bailey, M., Teeri, T. & Knowles, J. K. C. (1988) Efficient secretion of two fungal cellobiohydrolases in Saccharomyces cerevisiae, Gene (Amst.) 63, 103-112.
27. Raguz, S., Yague, E., Wood, D. A. & Thurston, C. F. (1992) Isolation and characterisation of a cellulose-growth-specific gene from Agaricus bisporus, Gene (Amst.) 119, 183-190.
28. Rouvinen, J., Bergfors, T., Teeri, T., Knowles, J. K. C. & Jones, T. A. (1990) Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei, Science 249, 380-386.
29. Sakon, J., Adney, W. S., Himmel, M. E., Thomas, S. R. & Karplus, P. A. (1996) Crystal structure of thermostabile family 5 endocellulase E1 from Acidothermus cellulolyticus in complex with cellotetraose, Biochemistry 35, 10648-10660.
30. Saloheimo, M., Lehtovaara, P., Penttilä, M., Teeri, T. T., Stahlberg, J., Johansson, G., Pettersson, G., Claeyssens, M., Tomme, P. & Knowles, J. (1988) EGIII, a new endoglucanase from Trichoderma reesei: the characterisation of both gene and enzyme, Gene (Amst.) 63, 11-21.
31. Saloheimo, A., Henrissat, B., Hoffrén, A.-M., Teleman, O. & Penttilä, M. (1994) A novel, small endoglucanase gene, eg15, from Trichoderma reesei isolated by expression in yeast, Mol. Microbiol. 13, 219-228.
32. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
33. Schmuck, M., Pilz, I., Hayn, M. & Esterbauer, H. (1986) Investigation of cellobiohydrolase from Trichoderma reesei by small angle X-ray scattering, Biotechnol. Lett. 8, 398-402.
34. Schülein, M., Tikhomirov, D. F. & Schou, C. (1993) Humicola insolens alkaline cellulases, in Proceedings of the second TRICEL symposium on Trichoderma reesei cellulases and other hydrolases (Suominen, P. & Reinikainen, T., eds) pp. 109-116, Foundation for Biotechnical and Industrial Fermentation Research, Helsinki.
35. Sherman, F. (1991) Getting started with yeast, Methods Enzymol. 194, 3-21.
36. Shoemaker, S., Schweickart, V., Ladner, M., Gelfand, D., Kwok, S., Myambo, K. & Innis, M. (1983) Molecular cloning of exo-cellobiohydrolase derived from Trichoderma reesei strain L27, Biotechnology 1, 691-695.
37. Stålbrand, H., Saloheimo, A., Vehmaanperä, J., Henrissat, B. & Penttilä, M. (1995) Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei β-mannanase gene containing a cellulose-binding domain, Appl. Environ. Microbiol. 61, 1090-1097.
38. Teeri, T., Salovuori, I. & Knowles, J. (1983) The molecular cloning of the major cellulase gene from Trichoderma reesei, Biotechnology 1, 696-699.
39. Teeri, T. T., Lehtovaara, P., Kauppinen, S., Salovuori, I. & Knowles, J. (1987) Homologous domains in Trichoderma reesei cellulolytic enzymes: gene sequence and expression of cellobiohydrolase II, Gene (Amst.) 51, 43-52.
40. van Arsdell, J. N., Kwok, S., Schweickart, V. L., Ladner, M. B., Gelfand, D. H. & Innis, M. A. (1987) Cloning, characterisation and expression in Saccharomyces cerevisiae of endoglucanase I from Trichoderma reesei, Biotechnology 5, 60-64.
41. von Heijne, G. (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14, 4683-4690.
42. Walseth, C. S. (1952) Occurrence of cellulases in enzyme preparations from micro-organisms, Tech. Assoc. Pulp Pap. Ind. 35, 228-233.
43. Ward, M., Wu, S., Dauberman, J., Weiss, G., Larenas, E., Bower, B., Rey, M., Clarkson, K. & Bott, R. (1993) Cloning, sequence and preliminary structural analysis of a small, high pI endoglucanase (EGIII) from Trichoderma reesei, in Proceedings of the second TRICEL symposium on Trichoderma reesei cellulases and other hydrolases (Suominen, P. & Reinikainen, T., eds) pp. 153-158, Foundation for Biotechnical and Industrial Fermentation Recearch, Helsinki.
44. Yague, E., Wood, D. A. & Thurston, C. F. (1994) Regulation of transcription of the cell gene in Agaricus bisporns, Mol. Microbiol. 12, 41-47.