Microarray analyses to quantify advantages of 2D and 3D hydrogel culture systems in maintaining the native valvular interstitial cell phenotype

Biomaterials

Volumn 74, Issue , 2016, Pages 31-41

  Mabry, Kelly M ^a   Payne, Samuel Z ^a   Anseth, Kristi S ^a,b  

^a UCB 450   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

ECM; Heart valve; Hydrogel; Microarray; Three dimensional cell culture; Valvular interstitial cells

Indexed keywords

CELL CULTURE; CELLS; CHEMICAL ACTIVATION; CYTOLOGY; GELS; GENE EXPRESSION; GENES; MICROARRAYS; MILITARY ELECTRONIC COUNTERMEASURES; POLYETHYLENE GLYCOLS; TISSUE; TISSUE CULTURE;

AORTIC STENOSIS; CULTURE SYSTEMS; EXPRESSION LEVELS; EXPRESSION PROFILE; HEART VALVES; MICROARRAY ANALYSIS; THREE DIMENSIONAL CELL CULTURE; TISSUE-CULTURE POLYSTYRENES;

HYDROGELS;

ALPHA SMOOTH MUSCLE ACTIN; MACROGOL; MESSENGER RNA; POLYSTYRENE; ACTIN; HYDROGEL;

ANIMAL CELL; ANIMAL TISSUE; AORTA STENOSIS; ARTICLE; CELL MOTILITY; CELL POLARITY; CELL STRUCTURE; GENE EXPRESSION; GENETIC TRANSCRIPTION; HEART VALVE; HYDROGEL; MICROARRAY ANALYSIS; MICROENVIRONMENT; MYOFIBROBLAST; NONHUMAN; PHENOTYPE; PIG; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; TISSUE CULTURE; VALVULAR INTERSTITIAL CELL; ANIMAL; CELL CULTURE TECHNIQUE; CYTOLOGY; METABOLISM;

ACTINS; ANIMALS; CELL CULTURE TECHNIQUES; HEART VALVES; HYDROGELS; MICROARRAY ANALYSIS; SWINE;

EID: 84945143652     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2015.09.035     Document Type: Article

References (56)

1. Durbin A.D., Gotlieb A.I. Advances towards understanding heart valve response to injury. Cardiovasc. Pathol. 2002, 11:69-77.
2. Liu A.C., Joag V.R., Gotlieb A.I. The emerging role of valve interstitial cell phenotypes in regulating heart valve pathobiology. Am. J. Pathol. 2007, 171:1407-1418.
3. Merryman W.D., Schoen F.J. Mechanisms of calcification in aortic valve disease: role of mechanokinetics and mechanodynamics. Curr. Cardiol. Rep. 2013, 15:355.
4. Lindman B.R., Bonow R.O., Otto C.M. Current management of calcific aortic stenosis. Circ. Res. 2013, 113:223-237.
5. Rabkin-Aikawa E., Farber M., Aikawa M., Schoen F.J. Dynamic and reversible changes of interstitial cell phenotype during remodeling of cardiac valves. J. Heart Valve Dis. 2004, 13:841-847.
6. Bowler M.A., Merryman W.D. In vitro models of aortic valve calcification: solidifying a system. Cardiovasc. Pathol. 2015, 24:1-10.
7. Miyake K., Satomi N., Sasaki S. Elastic modulus of polystyrene film from near surface to bulk measured by nanoindentation using atomic force microscopy. Appl. Phys. Lett. 2006, 89:18-21.
8. Wang H., Tibbitt M.W., Langer S.J., Leinwand L.A., Anseth K.S. Hydrogels preserve native phenotypes of valvular fibroblasts through an elasticity-regulated PI3K/AKT pathway. Proc. Natl. Acad. Sci. 2013, 110:19336-19341.
9. Hutcheson J.D., Setola V., Roth B.L., Merryman W.D. Serotonin receptors and heart valve disease-it was meant 2B. Pharmacol. Ther. 2011, 132:146-157.
10. Kloxin A.M., Benton J.A., Anseth K.S. In situ elasticity modulation with dynamic substrates to direct cell phenotype. Biomaterials 2010, 31:1-8.
11. Mabry K.M., Lawrence R.L., Anseth K.S. Dynamic stiffening of poly(ethylene glycol)-based hydrogels to direct valvular interstitial cell phenotype in a three-dimensional environment. Biomaterials 2015, 49:47-56.
12. Walker G.A., Masters K.S., Shah D.N., Anseth K.S., Leinwand L.A. Valvular myofibroblast activation by transforming growth factor-beta: implications for pathological extracellular matrix remodeling in heart valve disease. Circ. Res. 2004, 95:253-260.
13. Carabello B.A. Introduction to aortic stenosis. Circ. Res. 2013, 113:179-185.
14. Johnson C.M., Hanson M.N., Helgeson S.C. Porcine cardiac valvular subendothelial cells in culture: cell isolation and growth characteristics. J. Mol. Cell. Cardiol. 1987, 19:1185-1193.
15. Fairbanks B.D., Schwartz M.P., Halevi A.E., Nuttelman C.R., Bowman C.N., Anseth K.S. A versatile synthetic extracellular matrix mimic via thiol-norbornene photopolymerization. Adv. Mater. 2009, 21:5005-5010.
16. Benton J.A., Fairbanks B.D., Anseth K.S. Characterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogels. Biomaterials 2009, 30:6593-6603.
17. Bryant SJ, Anseth KS. Hydrogel Scaffolds n.d.
18. Huang D.W., Sherman B.T., Lempicki R.A. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat. Protoc. 2009, 4:44-57.
19. Huang D.W., Sherman B.T., Lempicki R.A. Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res. 2009, 37:1-13.
20. Mi H., Muruganujan A., Thomas P.D. PANTHER in 2013: modeling the evolution of gene function, and other gene attributes, in the context of phylogenetic trees. Nucleic Acids Res. 2013, 41:377-386.
21. Mi H., Thomas P. Protein networks and pathway analysis. Methods Mol. Biol. 2009, 563:123-140.
22. Farkas I.J., Szántó-Várnagy Á., Korcsmáros T. Linking proteins to signaling pathways for experiment design and evaluation. PLoS One 2012, 7:1-5.
23. Mabry KM, Payne SZ, Anseth KS. Transcriptomic changes in valvular interstitial cells in response to culture on tissue culture polystyrene, on 2D hydrogels, or within 3D hydrogels. Data in Brief, submitted.
24. Yip C.Y.Y., Chen J.-H., Zhao R., Simmons C.A. Calcification by valve interstitial cells is regulated by the stiffness of the extracellular matrix. Arterioscler. Thromb. Vasc. Biol. 2009, 29:936-942.
25. Gu X., Masters K.S. Regulation of valvular interstitial cell calcification by adhesive peptide sequences. J. Biomed. Mater. Res. A 2010, 93:1620-1630.
26. Gould S., Anseth K. Role of cell-matrix interactions on VIC phenotype and tissue deposition in 3D PEG hydrogels. J. Tissue Eng. Regener. Med. 2013, 10.1002/term.1836.
27. Fayet C., Bendeck M.P., Gotlieb A.I. Cardiac valve interstitial cells secrete fibronectin and form fibrillar adhesions in response to injury. Cardiovasc. Pathol. 2007, 16:203-211.
28. Duan B., Hockaday L.A., Kapetanovic E., Kang K.H., Butcher J.T. Stiffness and adhesivity control aortic valve interstitial cell behavior within hyaluronic acid based hydrogels. Acta Biomater. 2013, 9:7640-7650.
29. Cushing M.C., Liao J.-T., Anseth K.S. Activation of valvular interstitial cells is mediated by transforming growth factor-beta1 interactions with matrix molecules. Matrix Biol. 2005, 24:428-437.
30. Lu M., Munger J.S., Steadele M., Busald C., Tellier M., Schnapp L.M. Integrin alpha8beta1 mediates adhesion to LAP-TGFbeta1. J. Cell Sci. 2002, 115:4641-4648.
31. Bossé Y., Miqdad A., Fournier D., Pépin A., Pibarot P., Mathieu P. Refining molecular pathways leading to calcific aortic valve stenosis by studying gene expression profile of normal and calcified stenotic human aortic valves. Circ. Cardiovasc. Genet. 2009, 2:489-498.
32. O'Brien K.D. Pathogenesis of calcific aortic valve disease: a disease process comes of age (and a good deal more). Arterioscler. Thromb. Vasc. Biol. 2006, 26:1721-1728.
33. Eitzman D.T., McCoy R.D., Zheng X., Fay W.P., Shen T., Ginsburg D., et al. Bleomycin-induced pulmonary fibrosis in transgenic mice that either lack or overexpress the murine plasminogen activator inhibitor-1 gene. J. Clin. Invest. 1996, 97:232-237.
34. Edling C.E., Hallberg B. c-Kit-a hematopoietic cell essential receptor tyrosine kinase. Int. J. Biochem. Cell Biol. 2007, 39:1995-1998.
35. Miller J.D., Weiss R.M., Heistad D.D. Calcific aortic valve stenosis: methods, models, and mechanisms. Circ. Res. 2011, 108:1392-1412.
36. Miller J.D., Weiss R.M., Serrano K.M., Brooks R.M., Berry C.J., Zimmerman K., et al. Lowering plasma cholesterol levels halts progression of aortic valve disease in mice. Circulation 2009, 119:2693-2701.
37. Chen J.H., Simmons C.A. Cell-matrix interactions in the pathobiology of calcific aortic valve disease: critical roles for matricellular, matricrine, and matrix mechanics cues. Circ. Res. 2011, 108:1510-1524.
38. Chen J.-H., Chen W.L.K., Sider K.L., Yip C.Y.Y., Simmons C.A. B-catenin mediates mechanically regulated, transforming growth factor-B1-induced myofibroblast differentiation of aortic valve interstitial cells. Arterioscler. Thromb. Vasc. Biol. 2011, 31:590-597.
39. Hinderer S., Seifert J., Votteler M., Shen N., Rheinlaender J., Schäffer T.E., et al. Engineering of a bio-functionalized hybrid off-the-shelf heart valve. Biomaterials 2013, 35:2130-2139.
40. Nakayama Y., Takewa Y., Sumikura H., Yamanami M., Matsui Y., Oie T., et al. In-body tissue-engineered aortic valve (biovalve type VII) architecture based on 3D printer molding. J. Biomed. Mater. Res. B Appl. Biomater. 2014, 10-12.
41. Butcher J.T., Nerem R.M. Porcine aortic valve interstitial cells in three-dimensional culture: comparison of phenotype with aortic smooth muscle cells. J. Heart Valve Dis. 2004, 13:478-485. discussion 485-6.
42. Wei J., Han J., Zhao Y., Cui Y., Wang B., Xiao Z., et al. The importance of three-dimensional scaffold structure on stemness maintenance of mouse embryonic stem cells. Biomaterials 2014, 35:7724-7733.
43. Chambers K.F., Mosaad E.M.O., Russell P.J., Clements J.A., Doran M.R. 3D cultures of prostate cancer cells cultured in a novel high-throughput culture platform are more resistant to chemotherapeutics compared to cells cultured in monolayer. PLoS One 2014, 9:e111029.
44. DiMarco R.L., Su J., Yan K.S., Dewi R., Kuo C.J., Heilshorn S.C. Engineering of three-dimensional microenvironments to promote contractile behavior in primary intestinal organoids. Integr. Biol. (Camb) 2014, 6:127-142.
45. Huang Y., Hsu S. Acquisition of epithelial-mesenchymal transition and cancer stem-like phenotypes within chitosan-hyaluronan membrane-derived 3D tumor spheroids. Biomaterials 2014, 35:10070-10079.
46. Rodriguez K.J., Piechura L.M., Masters K.S. Regulation of valvular interstitial cell phenotype and function by hyaluronic acid in 2-D and 3-D culture environments. Matrix Biol. 2011, 30:70-82.
47. Butcher J.T., Nerem R.M. Valvular endothelial cells and the mechanoregulation of valvular pathology. Philos. Trans. R. Soc. Lond. B Biol. Sci. 2007, 362:1445-1457.
48. Butcher J.T., Nerem R.M. Valvular endothelial cells regulate the phenotype of interstitial cells in co-culture: effects of steady shear stress. Tissue Eng. 2006, 12:905-915.
49. Bernardini C., Greco F., Zannoni A., Bacci M.L., Seren E., Forni M. Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-induced apoptosis. J. Inflamm. (Lond.) 2012, 9:47.
50. Gould S.T., Matherly E.E., Smith J.N., Heistad D.D., Anseth K.S. The role of valvular endothelial cell paracrine signaling and matrix elasticity on valvular interstitial cell activation. Biomaterials 2014, 35:3596-3606.
51. Wipff P.-J., Rifkin D.B., Meister J.-J., Hinz B. Myofibroblast contraction activates latent TGF-beta1 from the extracellular matrix. J. Cell Biol. 2007, 179:1311-1323.
52. Schmidt D., Dijkman P.E., Driessen-Mol A., Stenger R., Mariani C., Puolakka A., et al. Minimally-invasive implantation of living tissue engineered heart valves: a comprehensive approach from autologous vascular cells to stem cells. J. Am. Coll. Cardiol. 2010, 56:510-520.
53. De Jonge H.J.M., Fehrmann R.S.N., de Bont E.S.J.M., Hofstra R.M.W., Gerbens F., Kamps W.A., et al. Evidence based selection of housekeeping genes. PLoS One 2007, 2:1-5.
54. Park C.Y., Zhou E.H., Tambe D., Chen B., Lavoie T., Dowell M., et al. High-throughput screening for modulators of cellular contractile force. Integr. Biol. 2015, 10.1039/c5ib00054h.
55. Burdick J.A., Anseth K.S. Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering. Biomaterials 2002, 23:4315-4323.
56. Hahn M.S., McHale M.K., Wang E., Schmedlen R.H., West J.L. Physiologic pulsatile flow bioreactor conditioning of poly(ethylene glycol)-based tissue engineered vascular grafts. Ann. Biomed. Eng. 2007, 35:190-200.