Novel computer vision algorithm for the reliable analysis of organelle morphology in whole cell 3D images - A pilot study for the quantitative evaluation of mitochondrial fragmentation in amyotrophic lateral sclerosis

Mitochondrion

Volumn 25, Issue , 2015, Pages 49-59

  Lautenschläger, Janin ^a   Lautenschläger, Christian ^a   Tadic, Vedrana ^a   Süße, Herbert ^b   Ortmann, Wolfgang ^b   Denzler, Joachim ^b   Stallmach, Andreas ^a   Witte, Otto W ^a   Grosskreutz, Julian ^a  

^a JENA UNIVERSITY HOSPITAL   (Germany)

^b FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Amyotrophic lateral sclerosis; Cell imaging; Mitochondria; Neurodegeneration; Organelle morphology; Organelle segmentation

Indexed keywords

COPPER ZINC SUPEROXIDE DISMUTASE; KAINIC ACID;

ALGORITHM; AMYOTROPHIC LATERAL SCLEROSIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL SIZE; CELL ULTRASTRUCTURE; CELLULAR PARAMETERS; COMPUTER ANALYSIS; CONTROLLED STUDY; EXCITOTOXICITY; FEMALE; FRACTAL ANALYSIS; HISTOGRAM; IMAGE ANALYSIS; MALE; MITOCHONDRIAL FRAGMENTATION; MITOCHONDRIAL VOLUME; MOTONEURON; MOUSE; NERVE CELL CULTURE; NONHUMAN; NUCLEAR SIZE; PILOT STUDY; POINT MUTATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RELIABILITY; THREE DIMENSIONAL IMAGING; TRANSGENIC MOUSE; WHOLE CELL; ANIMAL; BIOMETRY; DISEASE MODEL; EVALUATION STUDY; IMAGE PROCESSING; MITOCHONDRION; NERVE CELL; PATHOLOGY; PROCEDURES;

ALGORITHMS; AMYOTROPHIC LATERAL SCLEROSIS; ANIMALS; BIOMETRY; DISEASE MODELS, ANIMAL; EVALUATION STUDIES AS TOPIC; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; MICE, TRANSGENIC; MITOCHONDRIA; NEURONS; PILOT PROJECTS;

EID: 84944032009     PISSN: 15677249     EISSN: 18728278     Source Type: Journal    
DOI: 10.1016/j.mito.2015.10.003     Document Type: Article

References (46)

1. 2+ uptake in cystic fibrosis airway epithelial cells. Mitochondrion 2009, 9:232-241.
2. Chaturvedi R.K., Beal M.F. Mitochondria targeted therapeutic approaches in Parkinson's and Huntington's diseases. Mol. Cell. Neurosci. 2013, 55:101-114.
3. Cozzolino M., Ferri A., Valle C., Carri M.T. Mitochondria and ALS: implications from novel genes and pathways. Mol. Cell. Neurosci. 2013, 55:44-49.
4. Cribbs J.T., Strack S. Reversible phosphorylation of Drp1 by cyclic AMP-dependent protein kinase and calcineurin regulates mitochondrial fission and cell death. EMBO Rep. 2007, 8:939-944.
5. Damiano M., Galvan L., Deglon N., Brouillet E. Mitochondria in Huntington's disease. Biochim. Biophys. Acta 2010, 1802:52-61.
6. van Dis V., Kuijpers M., Haasdijk E.D., Teuling E., Oakes S.A., Hoogenraad C.C., Jaarsma D. Golgi fragmentation precedes neuromuscular denervation and is associated with endosome abnormalities in SOD1-ALS mouse motor neurons. Acta Neuropathol. Commun. 2014, 2:38.
7. Duffy L.M., Chapman A.L., Shaw P.J., Grierson A.J. Review: The role of mitochondria in the pathogenesis of amyotrophic lateral sclerosis. Neuropathol. Appl. Neurobiol. 2011, 37:336-352.
8. Eckert G.P., Renner K., Eckert S.H., Eckmann J., Hagl S., Abdel-Kader R.M., Kurz C., Leuner K., Muller W.E. Mitochondrial dysfunction-a pharmacological target in Alzheimer's disease. Mol. Neurobiol. 2012, 46:136-150.
9. Exner N., Lutz A.K., Haass C., Winklhofer K.F. Mitochondrial dysfunction in Parkinson's disease: molecular mechanisms and pathophysiological consequences. EMBO J. 2012, 31:3038-3062.
10. Fujita Y., Mizuno Y., Takatama M., Okamoto K. Anterior horn cells with abnormal TDP-43 immunoreactivities show fragmentation of the Golgi apparatus in ALS. J. Neurol. Sci. 2008, 269:30-34.
11. 2+ transients in cultured spinal motor neurons. Cell Calcium 2007, 42:59-69.
12. Grosskreutz J., Van Den Bosch L., Keller B.U. Calcium dysregulation in amyotrophic lateral sclerosis. Cell Calcium 2010, 47:165-174.
13. Gurney M.E., Pu H., Chiu A.Y., Dal Canto M.C., Polchow C.Y., Alexander D.D., Caliendo J., Hentati A., Kwon Y.W., Deng H.X., et al. Motor neuron degeneration in mice that express a human Cu,Zn superoxide dismutase mutation. Science (New York, N.Y.) 1994, 264:1772-1775.
14. Haastert K., Grosskreutz J., Jaeckel M., Laderer C., Bufler J., Grothe C., Claus P. Rat embryonic motoneurons in long-term co-culture with Schwann cells-a system to investigate motoneuron diseases on a cellular level in vitro. J. Neurosci. Methods 2005, 142:275-284.
15. Ivanovici M., Richard N., Decean H. Fractal dimension and lacunarity of psoriatic lesions-a colour approach. Medicine 2009, 6:7.
16. Joshi G., Chi Y., Huang Z., Wang Y. Abeta-induced Golgi fragmentation in Alzheimer's disease enhances Abeta production. Proc. Natl. Acad. Sci. U. S. A. 2014, 111:E1230-E1239.
17. Knott A.B., Perkins G., Schwarzenbacher R., Bossy-Wetzel E. Mitochondrial fragmentation in neurodegeneration. Nat. Rev. Neurosci. 2008, 9:505-518.
18. Körner M., Krishna M., Süße H., Ortmann W., Denzler J. Regularized geometric hulls for robust cell nucleus segmentation from microscopy images. Proceedings of the 18th Annual Conference in Medical Image Understanding and Analysis (MIUA) 2014, 117-122.
19. Kucharz K., Wieloch T., Toresson H. Fission and fusion of the neuronal endoplasmic reticulum. Transl. Stroke Res. 2013, 4:652-662.
20. Lautenschläger J., Prell T., Grosskreutz J. Endoplasmic reticulum stress and the ER mitochondrial calcium cycle in amyotrophic lateral sclerosis. Amyotroph. Lateral Scler. 2012, 13:166-177.
21. Lautenschläger J., Prell T., Ruhmer J., Weidemann L., Witte O.W., Grosskreutz J. Overexpression of human mutated G93A SOD1 changes dynamics of the ER mitochondria calcium cycle specifically in mouse embryonic motor neurons. Exp. Neurol. 2013, 247:91-100.
22. Lin B., Yang Z. A suggested lacunarity expression for Sierpinski carpets. J. Phys. A Math. Gen. 1986, 19.
23. Luo G., Yi J., Ma C., Xiao Y., Yi F., Yu T., Zhou J. Defective mitochondrial dynamics is an early event in skeletal muscle of an amyotrophic lateral sclerosis mouse model. PLoS One 2013, 8:e82112.
24. Magrane J., Hervias I., Henning M.S., Damiano M., Kawamata H., Manfredi G. Mutant SOD1 in neuronal mitochondria causes toxicity and mitochondrial dynamics abnormalities. Hum. Mol. Genet. 2009, 18:4552-4564.
25. Mandelbrot B.B. The Fractal Geometry of Nature/Revised and Enlarged Edition 1983, 495. WH Freeman and Co., New York, (p. 1).
26. Martel C., Huynh le H., Garnier A., Ventura-Clapier R., Brenner C. Inhibition of the mitochondrial permeability transition for cytoprotection: direct versus indirect mechanisms. Biochem. Res. Int. 2012, 2012:213403.
27. Mourelatos Z., Gonatas N.K., Stieber A., Gurney M.E., Dal Canto M.C. The Golgi apparatus of spinal cord motor neurons in transgenic mice expressing mutant Cu,Zn superoxide dismutase becomes fragmented in early, preclinical stages of the disease. Proc. Natl. Acad. Sci. U. S. A. 1996, 93:5472-5477.
28. 2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem. Biol. 2006, 13:521-530.
29. Pasantes-Morales H., Tuz K. Volume changes in neurons: hyperexcitability and neuronal death. Contrib. Nephrol. 2006, 152:221-240.
30. 2+ uptake. Nature 2010, 467:291-296.
31. Raimondi A., Mangolini A., Rizzardini M., Tartari S., Massari S., Bendotti C., Francolini M., Borgese N., Cantoni L., Pietrini G. Cell culture models to investigate the selective vulnerability of motoneuronal mitochondria to familial ALS-linked G93ASOD1. Eur. J. Neurosci. 2006, 24:387-399.
32. Reddy P.H. Amyloid beta, mitochondrial structural and functional dynamics in Alzheimer's disease. Exp. Neurol. 2009, 218:286-292.
33. Reddy P.H., Reddy T.P. Mitochondria as a therapeutic target for aging and neurodegenerative diseases. Curr. Alzheimer Res. 2011, 8:393-409.
34. Rodner E., Süße H., Ortmann W., Denzler J. Difference of boxes filters revisited: shadow suppression and efficient character segmentation. Proceedings of the Eighth IAPR Workshop on Document Analysis Systems 2008, 263-269.
35. Sengupta D., Linstedt A.D. Control of organelle size: the Golgi complex. Annu. Rev. Cell Dev. Biol. 2011, 27:57-77.
36. Song W., Bossy B., Martin O.J., Hicks A., Lubitz S., Knott A.B., Bossy-Wetzel E. Assessing mitochondrial morphology and dynamics using fluorescence wide-field microscopy and 3D image processing. Methods (San Diego, Calif.) 2008, 46:295-303.
37. Song W., Chen J., Petrilli A., Liot G., Klinglmayr E., Zhou Y., Poquiz P., Tjong J., Pouladi M.A., Hayden M.R., Masliah E., Ellisman M., Rouiller I., Schwarzenbacher R., Bossy B., Perkins G., Bossy-Wetzel E. Mutant huntingtin binds the mitochondrial fission GTPase dynamin-related protein-1 and increases its enzymatic activity. Nat. Med. 2011, 17:377-382.
38. Song W., Song Y., Kincaid B., Bossy B., Bossy-Wetzel E. Mutant SOD1G93A triggers mitochondrial fragmentation in spinal cord motor neurons: neuroprotection by SIRT3 and PGC-1alpha. Neurobiol. Dis. 2013, 51:72-81.
39. Stieber A., Gonatas J.O., Moore J.S., Bantly A., Yim H.S., Yim M.B., Gonatas N.K. Disruption of the structure of the Golgi apparatus and the function of the secretory pathway by mutants G93A and G85R of Cu, Zn superoxide dismutase (SOD1) of familial amyotrophic lateral sclerosis. J. Neurol. Sci. 2004, 219:45-53.
40. Su B., Wang X., Bonda D., Perry G., Smith M., Zhu X. Abnormal mitochondrial dynamics-a novel therapeutic target for Alzheimer's disease?. Mol. Neurobiol. 2010, 41:87-96.
41. Süße H., Ortmann W., Lautenschläger J., Lautenschläger C., Körner M., Grosskreutz J., Denzler J. Quantitative analysis of pathological mitochondrial morphology in neuronal cells in confocal laser scanning microscopy images. Proceedings of the 2nd International Work-Conference on Bioinformatics and Biomedical Engineering (IWBBIO) 2014, 1290-1301.
42. Szendroedi J., Phielix E., Roden M. The role of mitochondria in insulin resistance and type 2 diabetes mellitus. Nat. Rev. Endocrinol. 2012, 8:92-103.
43. 2+-dependent AMPA receptor-mediated motoneuron death. J. Neurosci. 2003, 23:4942-4950.
44. Voeltz G.K., Prinz W.A. Sheets, ribbons and tubules - how organelles get their shape. Nat. Rev. Mol. Cell Biol. 2007, 8:258-264.
45. Wallace D.C. Mitochondria and cancer. Nat. Rev. Cancer 2012, 12:685-698.
46. Youle R.J., van der Bliek A.M. Mitochondrial fission, fusion, and stress. Science (New York, N.Y.) 2012, 337:1062-1065.