Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector-Coding Sequence Junction

ACS Synthetic Biology

Volumn 4, Issue 9, 2015, Pages 959-965

  Mirzadeh, Kiavash ^a   Martínez, Virginia ^b   Toddo, Stephen ^a   Guntur, Suchithra ^a   Herrgård, Markus J ^b   Elofsson, Arne ^a   Nørholm, Morten H H ^b   Daley, Daniel O ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b Chalmers University of Technology   (Denmark)

Author keywords

codon optimization; membrane protein; mRNA structure; protein production; translation initiation; vector coding sequence junction

Indexed keywords

BACTERIAL PROTEIN; MESSENGER RNA; BACTERIAL DNA; START CODON;

ARTICLE; CLONING; CODING; DNA BASE COMPOSITION; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PURIFICATION; TRANSLATION INITIATION; DNA SEQUENCE; GENE VECTOR; GENETICS; HIGH THROUGHPUT SEQUENCING; METABOLISM; MOLECULAR CLONING; PLASMID; PROTEIN SYNTHESIS; RNA STABILITY; RNA TRANSLATION; START CODON;

CLONING, MOLECULAR; CODON, INITIATOR; DNA, BACTERIAL; ESCHERICHIA COLI; GENETIC VECTORS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; PEPTIDE CHAIN INITIATION, TRANSLATIONAL; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; RNA STABILITY; SEQUENCE ANALYSIS, DNA;

EID: 84942052974     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.5b00033     Document Type: Article

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