A translation-coupling DNA cassette for monitoring protein translation in Escherichia coli

Metabolic Engineering

Volumn 14, Issue 4, 2012, Pages 298-305

  Mendez Perez, Daniel ^a   Gunasekaran, Suman ^a,b   Orler, Victor J ^a   Pfleger, Brian F ^a  

^a University of Wisconsin Madison   (United States)

^b James Madison Memorial High School   (United States)

Author keywords

Escherichia coli; MRNA secondary structure; Protein expression; Synthetic biology; Translational coupling

Indexed keywords

ANTIBIOTIC RESISTANCE; C-TERMINAL DOMAINS; E. COLI; FLUORESCENT PROTEIN; HETEROLOGOUS EXPRESSION; LOW LEVEL; MULTI-DOMAIN ENZYMES; PROTEIN EXPRESSIONS; PROTEIN TRANSLATION; RESPONSE SIGNAL; SECONDARY STRUCTURES; SEQUENCE VARIANTS; SYNTHETIC BIOLOGY; TARGET GENES; TRANSLATIONAL COUPLING;

ESCHERICHIA COLI; EXPERIMENTS; GENE EXPRESSION; NUCLEIC ACIDS; OPTIMIZATION;

PROTEINS;

DNA; MESSENGER RNA; PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; EARLY RESPONSE GENE; ESCHERICHIA COLI; GENE EXPRESSION; GENE PRODUCT; MONITORING; NONHUMAN; PRIORITY JOURNAL; TRANSLATION INITIATION;

DNA; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; METABOLIC ENGINEERING; OLIGONUCLEOTIDES; PLASMIDS; PROTEIN BIOSYNTHESIS;

ESCHERICHIA COLI;

EID: 84862169385     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2012.04.005     Document Type: Article

References (27)

1. Aksoy S., Squires C.L., Squires C. Translational coupling of the trpB and trpA genes in the Escherichia coli tryptophan operon. J. Bacteriol. 1984, 157:363-367.
2. Amann E., Ochs B., Abel K.J. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene 1988, 69:301-315.
3. Angov E., Hillier C.J., Kincaid R.L., Lyon J.A. Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression host. PLoS One 2008, 3:e2189.
4. Aslanidis C., de Jong P.J. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 1990, 18:6069-6074.
5. Boyle, P.M., Silver, P.A., 2012. Parts plus pipes: synthetic biology approaches to metabolic engineering. Metab. Eng.
6. Carlson R. The changing economics of DNA synthesis. Nat. Biotechnol. 2009, 27:1091-1094.
7. Gustafsson C., Govindarajan S., Minshull J. Codon bias and heterologous protein expression. Trends Biotechnol. 2004, 22:346-353.
8. Ivey-Hoyle M., Steege D.A. Translation of phage f1 gene VII occurs from an inherently defective initiation site made functional by coupling. J. Mol. Biol. 1989, 208:233-244.
9. Kane J.F. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr. Opin. Biotechnol. 1995, 6:494-500.
10. Keasling J.D. Synthetic biology for synthetic chemistry. ACS Chem. Biol. 2008, 3:64-76.
11. Keasling J.D. Manufacturing molecules through metabolic engineering. Science 2010, 330:1355-1358.
12. Keasling, J.D., 2012. Synthetic biology and the development of tools for metabolic engineering. Metab. Eng.
13. Li S.C., Squires C.L., Squires C. Antitermination of E. coli rRNA transcription is caused by a control region segment containing lambda nut-like sequences. Cell 1984, 38:851-860.
14. Lindahl L., Archer R.H., McCormick J.R., Freedman L.P., Zengel J.M. Translational coupling of the two proximal genes in the S10 ribosomal protein operon of Escherichia coli. J. Bacteriol. 1989, 171:2639-2645.
15. Lindahl L., Zengel J.M. Ribosomal genes in Escherichia coli. Annu. Rev. Genet. 1986, 20:297-326.
16. Mendez-Perez D., Begemann M.B., Pfleger B.F. Modular synthase-encoding gene involved in alpha-olefin biosynthesis in Synechococcus sp. strain PCC 7002. Appl. Environ. Microbiol. 2011, 77:4264-4267.
17. Michener, J.K., Thodey, K., Liang, J.C., Smolke, C.D., 2012. Applications of genetically-encoded biosensors for the construction and control of biosynthetic pathways. Metab. Eng.
18. Mutka S.C., Carney J.R., Liu Y., Kennedy J. Heterologous production of epothilone C and D in Escherichia coli. Biochemistry 2006, 45:1321-1330.
19. Oliver G., Gosset G., Sanchez-Pescador R., Lozoya E., Ku L.M., Flores N., Becerril B., Valle F., Bolivar F. Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12. Gene 1987, 60:1-11.
20. Oppenheim D.S., Yanofsky C. Translational coupling during expression of the tryptophan operon of Escherichia coli. Genetics 1980, 95:785-795.
21. Pfeifer B.A., Khosla C. Biosynthesis of polyketides in heterologous hosts. Microbiol. Mol. Biol. Rev. 2001, 65:106. -+.
22. Pfleger B.F., Fawzi N.J., Keasling J.D. Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiency. Biotechnol. Bioeng. 2005, 92:553-558.
23. Salis H.M., Mirsky E.A., Voigt C.A. Automated design of synthetic ribosome binding sites to control protein expression. Nat. Biotechnol. 2009, 27:946-950.
24. Schumperli D., McKenney K., Sobieski D.A., Rosenberg M. Translational coupling at an intercistronic boundary of the Escherichia coli galactose operon. Cell 1982, 30:865-871.
25. Takyar S., Hickerson R.P., Noller H.F. mRNA helicase activity of the ribosome. Cell 2005, 120:49-58.
26. Tyo K.E., Alper H.S., Stephanopoulos G.N. Expanding the metabolic engineering toolbox: more options to engineer cells. Trends Biotechnol. 2007, 25:132-137.
27. Xu Y., Alvey R.M., Byrne P.O., Graham J.E., Shen G., Bryant D.A. Expression of genes in cyanobacteria: adaptation of endogenous plasmids as platforms for high-level gene expression in Synechococcus sp. PCC 7002. Methods Mol. Biol. 2011, 684:273-293.