Improved production of membrane proteins in escherichia coli by selective codon substitutions

FEBS Letters

Volumn 587, Issue 15, 2013, Pages 2352-2358

  Nørholm, Morten H H ^a,b   Toddo, Stephen ^a   Virkki, Minttu T I ^a   Light, Sara ^a   Von Heijne, Gunnar ^a   Daley, Daniel O ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b Chalmers University of Technology   (Denmark)

Author keywords

AraH; Codon optimization; Escherichia coli; Membrane protein; NarK; Over expression; Synthetic coding sequence; Transporter

Indexed keywords

MEMBRANE PROTEIN; PROTEIN ARAH; PROTEIN NARK; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARAH GENE; ARTICLE; CODON SUBSTITUTION; ESCHERICHIA COLI; GENETIC ENGINEERING; GENETIC MANIPULATION; NARK GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; START CODON;

ARAH; CODON OPTIMIZATION; ESCHERICHIA COLI; MEMBRANE PROTEIN; NARK; OVER-EXPRESSION; SYNTHETIC CODING SEQUENCE; TRANSPORTER;

AMINO ACID SEQUENCE; BASE SEQUENCE; CODON; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID;

ESCHERICHIA COLI;

EID: 84880703203     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2013.05.063     Document Type: Article

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