The nucleotide composition of the spacer sequence influences the expression yield of heterologously expressed genes in Bacillus subtilis

Journal of Biotechnology

Volumn 191, Issue , 2014, Pages 214-220

  Liebeton, Klaus ^a   Lengefeld, Jette ^b   Eck, Jürgen ^a  

^a BRAIN AG   (Germany)

^b ETH ZURICH   (Switzerland)

Author keywords

Bacillus subtilis; Folding of transcript; Heterologous expression; Spacer sequence; Translation initiation

Indexed keywords

BACTERIOLOGY; ENZYMES; NUCLEOTIDES; RNA;

BACILLUS SUBTILIS; EXPRESSED GENES; EXPRESSION LEVELS; FOLDING OF TRANSCRIPT; HETEROLOGOUS EXPRESSION; NUCLEOTIDE COMPOSITION; SECONDARY STRUCTURES; SPACER SEQUENCE;

GENE EXPRESSION;

BACTERIAL ENZYME; LACCASE; LACCASE COTA; PROTEASE H149; PROTEINASE; UNCLASSIFIED DRUG; NUCLEOTIDE; PEPTIDE HYDROLASE; RIBOSOMAL SPACER DNA; RNA 16S;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; CLONING; CONTROLLED STUDY; ENZYME ACTIVITY; GENE LIBRARY; GENETIC SCREENING; HETEROLOGOUS EXPRESSION; HYDROGEN BOND; METAGENOME; NONHUMAN; NUCLEOTIDE COMPOSITION; NUCLEOTIDE SEQUENCE; PROTEIN SECONDARY STRUCTURE; START CODON; TRANSLATION INITIATION; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; PROMOTER REGION;

BACILLUS SUBTILIS;

BACILLUS SUBTILIS; DNA, RIBOSOMAL SPACER; GENE EXPRESSION REGULATION, BACTERIAL; METAGENOME; NUCLEOTIDES; PEPTIDE HYDROLASES; PROMOTER REGIONS, GENETIC; RNA, RIBOSOMAL, 16S; TRANSCRIPTION, GENETIC;

EID: 84911944425     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.06.027     Document Type: Article

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