Erratum: Accumulation of mitochondrial DNA mutations disrupts cardiac progenitor cell function and reduces survival (Journal of Biological Chemistry (2015) 290 (22061-22075) DOI: 10.1074/jbc.M115.649657);Accumulation of mitochondrial DNA mutations disrupts cardiac progenitor cell function and reduces survival

Journal of Biological Chemistry

Volumn 290, Issue 36, 2015, Pages 22061-22075

  Orogo, Amabel M ^a   Gonzalez, Eileen R ^a   Kubli, Dieter A ^a   Baptista, Igor L ^a   Ong, Sang Bing ^a   Prolla, Tomas A ^d   Sussman, Mark A ^c   Murphy, Anne N ^b   Gustafsson, Åsa B ^a,b  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b San Diego   (United States)

^c SAN DIEGO STATE UNIVERSITY   (United States)

^d UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; DNA; NUCLEIC ACIDS;

CARDIAC PROGENITORS; CELL THERAPY; MITOCHONDRIAL DNA (MTDNA); MITOCHONDRIAL DNA MUTATION; MITOCHONDRIAL FUNCTION; PROGENITOR CELL;

CELLS;

CD31 ANTIGEN; DRP1 PROTEIN; MITOCHONDRIAL DNA; MITOCHONDRIAL PROTEIN; MITOFUSIN 1; MITOFUSIN 2; MYOD PROTEIN; NERVE CELL ADHESION MOLECULE; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA COACTIVATOR 1ALPHA; TRANSCRIPTION FACTOR GATA 4; TRANSCRIPTION FACTOR GATA 6; UNCLASSIFIED DRUG; CYTOCHROME C OXIDASE; DNA DIRECTED DNA POLYMERASE; POLG PROTEIN, MOUSE;

ADULT; ANIMAL CELL; ARTICLE; CARDIAC STEM CELL; CELL DEATH; CELL DIFFERENTIATION; CELL DIVISION; CELL EXPANSION; CELL FUNCTION; CELL PROLIFERATION; CELL RENEWAL; CELL SIZE; CELL SURVIVAL; CONTROLLED STUDY; GLYCOLYSIS; MALE; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRIAL RESPIRATION; MITOPHAGY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE PHOSPHORYLATION; PRIORITY JOURNAL; UPREGULATION; ANIMAL; C57BL MOUSE; CARDIAC MUSCLE; CELL CULTURE; CYTOLOGY; FEMALE; FLUORESCENCE MICROSCOPY; GENETICS; KNOCKOUT MOUSE; METABOLISM; MITOCHONDRION; MUTATION; ORGANELLE BIOGENESIS; OXYGEN CONSUMPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEM CELL; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; DNA, MITOCHONDRIAL; DNA-DIRECTED DNA POLYMERASE; ELECTRON TRANSPORT COMPLEX IV; FEMALE; MALE; MICE, INBRED C57BL; MICE, KNOCKOUT; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; MUTATION; MYOCARDIUM; ORGANELLE BIOGENESIS; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELLS;

EID: 84941358810     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.A115.649657     Document Type: Erratum

References (62)

1. Leri, A., Kajstura, J., and Anversa, P. (2011) Role of cardiac stem cells in cardiac pathophysiology: A paradigm shift in human myocardial biology. Circ. Res. 109, 941-961.
2. Beltrami, A. P., Barlucchi, L., Torella, D., Baker, M., Limana, F., Chimenti, S., Kasahara, H., Rota, M., Musso, E., Urbanek, K., Leri, A., Kajstura, J., Nadal-Ginard, B., and Anversa, P. (2003) Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell 114, 763-776.
3. Ellison, G. M., Vicinanza, C., Smith, A. J., Aquila, I., Leone, A., Waring, C. D., Henning, B. J., Stirparo, G. G., Papait, R., Scarfò, M., Agosti, V., Viglietto, G., Condorelli, G., Indolfi, C., Ottolenghi, S., Torella, D., and Nadal-Ginard, B. (2013) Adult c-kit(pos) cardiac stem cells are necessary and sufficient for functional cardiac regeneration and repair. Cell 154, 827-842.
4. van Berlo, J. H., Kanisicak, O., Maillet, M., Vagnozzi, R. J., Karch, J., Lin, S. C., Middleton, R. C., Marbán, E., and Molkentin, J. D. (2014) c-kit+cells minimally contribute cardiomyocytes to the heart. Nature 509, 337-341.
5. Senyo, S. E., Steinhauser, M. L., Pizzimenti, C. L., Yang, V. K., Cai, L., Wang, M., Wu, T. D., Guerquin-Kern, J. L., Lechene, C. P., and Lee, R. T. (2013) Mammalian heart renewal by pre-existing cardiomyocytes. Nature 493, 433-436.
6. Li, Q., Guo, Y., Ou, Q., Chen, N., Wu, W. J., Yuan, F., O'Brien, E., Wang, T., Luo, L., Hunt, G. N., Zhu, X., and Bolli, R. (2011) Intracoronary administration of cardiac stem cells in mice: A new, improved technique for cell therapy in murine models. Basic Res. Cardiol. 106, 849-864.
7. Fischer, K. M., Cottage, C. T., Wu, W., Din, S., Gude, N. A., Avitabile, D., Quijada, P., Collins, B. L., Fransioli, J., and Sussman, M. A. (2009) Enhancement of myocardial regeneration through genetic engineering of cardiac progenitor cells expressing Pim-1 kinase. Circulation 120, 2077-2087.
8. De Angelis, A., Piegari, E., Cappetta, D., Marino, L., Filippelli, A., Berrino, L., Ferreira-Martins, J., Zheng, H., Hosoda, T., Rota, M., Urbanek, K., Kajstura, J., Leri, A., Rossi, F., and Anversa, P. (2010) Anthracycline car- diomyopathy is mediated by depletion of the cardiac stem cell pool and is rescued by restoration of progenitor cell function. Circulation 121, 276-292.
9. Bolli, R., Chugh, A. R., D'Amario, D., Loughran, J. H., Stoddard, M. F., Ikram, S., Beache, G. M., Wagner, S. G., Leri, A., Hosoda, T., Sanada, F., Elmore, J. B., Goichberg, P., Cappetta, D., Solankhi, N. K., Fahsah, I., Rokosh, D. G., Slaughter, M. S., Kajstura, J., and Anversa, P. (2011) Cardiac stem cells in patients with ischaemic cardiomyopathy (SCIPIO): initial results of a randomised phase 1 trial. Lancet 378, 1847-1857.
10. Chugh, A. R., Beache, G. M., Loughran, J. H., Mewton, N., Elmore, J. B., Kajstura, J., Pappas, P., Tatooles, A., Stoddard, M. F., Lima, J. A., Slaughter, M. S., Anversa, P., and Bolli, R. (2012) Administration of cardiac stem cells in patients with ischemic cardiomyopathy: The SCIPIO trial: surgical aspects and interim analysis of myocardial function and viability by magnetic resonance. Circulation 126, S54-S64.
11. Mohsin, S., Khan, M., Nguyen, J., Alkatib, M., Siddiqi, S., Hariharan, N., Wallach, K., Monsanto, M., Gude, N., Dembitsky, W., and Sussman, M. A. (2013) Rejuvenation of human cardiac progenitor cells with Pim-1 kinase. Circ. Res. 113, 1169-1179.
12. Hariharan, N., Quijada, P., Mohsin, S., Joyo, A., Samse, K., Monsanto, M., De La Torre, A., Avitabile, D., Ormachea, L., McGregor, M. J., Tsai, E. J., and Sussman, M. A. (2015) Nucleostemin rejuvenates cardiac progenitor cells and antagonizes myocardial aging. J. Am. Coll. Cardiol. 65, 133-147.
13. Goichberg, P., Kannappan, R., Cimini, M., Bai, Y., Sanada, F., Sorrentino, A., Signore, S., Kajstura, J., Rota, M., Anversa, P., and Leri, A. (2013) Ageassociated defects in EphA2 signaling impair the migration of human cardiac progenitor cells. Circulation 128, 2211-2223.
14. Nunnari, J., and Suomalainen, A. (2012) Mitochondria: in sickness and in health. Cell 148, 1145-1159.
15. Atamna, H. (2004) Heme, iron, and the mitochondrial decay of ageing. Ageing Res. Rev. 3, 303-318.
16. Xu, X., Duan, S., Yi, F., Ocampo, A., Liu, G. H., and Izpisua Belmonte, J. C. (2013) Mitochondrial regulation in pluripotent stem cells. Cell Metab. 18, 325-332.
17. Dai, D. F., Santana, L. F., Vermulst, M., Tomazela, D. M., Emond, M. J., MacCoss, M. J., Gollahon, K., Martin, G. M., Loeb, L. A., Ladiges, W. C., and Rabinovitch, P. S. (2009) Overexpression of catalase targeted to mitochondria attenuates murine cardiac aging. Circulation 119, 2789-2797.
18. Corral-Debrinski, M., Horton, T., Lott, M. T., Shoffner, J. M., Beal, M. F., and Wallace, D. C. (1992) Mitochondrial DNA deletions in human brain: Regional variability and increase with advanced age. Nat. Genet. 2, 324-329.
19. Wanagat, J., Wolff, M. R., and Aiken, J. M. (2002) Age-associated changes in function, structure and mitochondrial genetic and enzymatic abnormalities in the Fischer 344 x Brown Norway F(1) hybrid rat heart. J. Mol. Cell Cardiol. 34, 17-28.
20. Khaidakov, M., Heflich, R. H., Manjanatha, M. G., Myers, M. B., and Aidoo, A. (2003) Accumulation of point mutations in mitochondrialDNAof aging mice. Mutat. Res. 526, 1-7.
21. Bratic, A., and Larsson, N. G. (2013) The role of mitochondria in aging. J. Clin. Invest. 123, 951-957.
22. Lebrecht, D., and Walker, U. A. (2007) Role of mtDNA lesions in anthracycline cardiotoxicity. Cardiovasc. Toxicol. 7, 108-113.
23. Frerichs, F. C., Dingemans, K. P., and Brinkman, K. (2002) Cardiomyopathy with mitochondrial damage associated with nucleoside reverse-transcriptase inhibitors. N. Engl. J. Med. 347, 1895-1896.
24. Ide, T., Tsutsui, H., Hayashidani, S., Kang, D., Suematsu, N., Nakamura, K., Utsumi, H., Hamasaki, N., and Takeshita, A. (2001) Mitochondrial DNA damage and dysfunction associated with oxidative stress in failing hearts after myocardial infarction. Circ. Res. 88, 529-535.
25. Kujoth, G. C., Hiona, A., Pugh, T. D., Someya, S., Panzer, K., Wohlgemuth, S. E., Hofer, T., Seo, A. Y., Sullivan, R., Jobling, W. A., Morrow, J. D., Van Remmen, H., Sedivy, J. M., Yamasoba, T., Tanokura, M., Weindruch, R., Leeuwenburgh, C., and Prolla, T. A. (2005) Mitochondrial DNA mutations, oxidative stress, and apoptosis in mammalian aging. Science 309, 481-484.
26. Trifunovic, A., Wredenberg, A., Falkenberg, M., Spelbrink, J. N., Rovio, A. T., Bruder, C. E., Bohlooly-Y, M., Gidlöf, S., Oldfors, A., Wibom, R., Törnell, J., Jacobs, H. T., and Larsson, N. G. (2004) Premature ageing in mice expressing defective mitochondrial DNA polymerase. Nature 429, 417-423.
27. Dai, D. F., Chen, T., Wanagat, J., Laflamme, M., Marcinek, D. J., Emond, M. J., Ngo, C. P., Prolla, T. A., and Rabinovitch, P. S. (2010) Age-dependent cardiomyopathy in mitochondrial mutator mice is attenuated by overexpression of catalase targeted to mitochondria. Aging Cell 9, 536-544.
28. Wang, W., Osenbroch, P., Skinnes, R., Esbensen, Y., Bjørås, M., and Eide, L. (2010) MitochondrialDNAintegrity is essential for mitochondrial maturation during differentiation of neural stem cells. Stem Cells 28, 2195-2204.
29. Chung, S., Dzeja, P. P., Faustino, R. S., Perez-Terzic, C., Behfar, A., and Terzic, A. (2007) Mitochondrial oxidative metabolism is required for the cardiac differentiation of stem cells Nat. Clin. Pract. Cardiovasc. Med. 4, S60-S67.
30. Chen, C. T., Shih, Y. R., Kuo, T. K., Lee, O. K., and Wei, Y. H. (2008) Coordinated changes of mitochondrial biogenesis and antioxidant enzymes during osteogenic differentiation of human mesenchymal stem cells. Stem Cells 26, 960-968.
31. Spitkovsky, D., Sasse, P., Kolossov, E., Böttinger, C., Fleischmann, B. K., Hescheler, J., and Wiesner, R. J. (2004) Activity of complex III of the mitochondrial electron transport chain is essential for early heart muscle cell differentiation. FASEB J. 18, 1300-1302.
32. Chung, S., Arrell, D. K., Faustino, R. S., Terzic, A., and Dzeja, P. P. (2010) Glycolytic network restructuring integral to the energetics of embryonic stem cell cardiac differentiation. J. Mol. Cell Cardiol. 48, 725-734.
33. Fransioli, J., Bailey, B., Gude, N. A., Cottage, C. T., Muraski, J. A., Emmanuel, G., Wu, W., Alvarez, R., Rubio, M., Ottolenghi, S., Schaefer, E., and Sussman, M. A. (2008) Evolution of the c-kit-positive cell response to pathological challenge in the myocardium. Stem Cells 26, 1315-1324.
34. Luchessi, A. D., Cambiaghi, T. D., Hirabara, S. M., Lambertucci, R. H., Silveira, L. R., Baptista, I. L., Moriscot, A. S., Costa-Neto, C. M., and Curi, R. (2009) Involvement of eukaryotic translation initiation factor 5A (eIF5A) in skeletal muscle stem cell differentiation. J. Cell. Physiol. 218, 480-489.
35. Kubli, D. A., Zhang, X., Lee, Y., Hanna, R. A., Quinsay, M. N., Nguyen, C. K., Jimenez, R., Petrosyan, S., Murphy, A. N., and Gustafsson, A. B. (2013) Parkin protein deficiency exacerbates cardiac injury and reduces survival following myocardial infarction. J. Biol. Chem. 288, 915-926.
36. Rikka, S., Quinsay, M. N., Thomas, R. L., Kubli, D. A., Zhang, X., Murphy, A. N., and Gustafsson, Å. B. (2011) Bnip3 impairs mitochondrial bioenergetics and stimulates mitochondrial turnover. Cell Death Differ. 18, 721-731.
37. Hanna, R. A., Quinsay, M. N., Orogo, A. M., Giang, K., Rikka, S., and Gustafsson, Å. B. (2012) Microtubule-associated protein 1 light chain 3 (LC3) Interacts with Bnip3 protein to selectively remove endoplasmic reticulum and mitochondria via autophagy. J. Biol. Chem. 287, 19094-19104.
38. Linke, A., Müller, P., Nurzynska, D., Casarsa, C., Torella, D., Nascimbene, A., Castaldo, C., Cascapera, S., Böhm, M., Quaini, F., Urbanek, K., Leri, A., Hintze, T. H., Kajstura, J., and Anversa, P. (2005) Stem cells in the dog heart are self-renewing, clonogenic, and multipotent and regenerate infarcted myocardium, improving cardiac function. Proc. Natl. Acad. Sci. U.S.A. 102, 8966-8971.
39. Bearzi, C., Rota, M., Hosoda, T., Tillmanns, J., Nascimbene, A., De Angelis, A., Yasuzawa-Amano, S., Trofimova, I., Siggins, R. W., Lecapitaine, N., Cascapera, S., Beltrami, A. P., D'Alessandro, D. A., Zias, E., Quaini, F., Urbanek, K., Michler, R. E., Bolli, R., Kajstura, J., Leri, A., and Anversa, P. (2007) Human cardiac stem cells. Proc. Natl. Acad. Sci. U.S.A. 104, 14068-14073.
40. Kasahara, A., and Scorrano, L. (2014) Mitochondria: from cell death executioners to regulators of cell differentiation. Trends Cell Biol. 24, 761-770.
41. Shefer, G., Van de Mark, D. P., Richardson, J. B., and Yablonka-Reuveni, Z. (2006) Satellite-cell pool size does matter: defining the myogenic potency of aging skeletal muscle. Dev. Biol. 294, 50-66.
42. Shavlakadze, T., McGeachie, J., and Grounds, M. D. (2010) Delayed but excellent myogenic stem cell response of regenerating geriatric skeletal muscles in mice. Biogerontology 11, 363-376.
43. Hatefi, Y. (1985)Themitochondrial electron transport and oxidative phosphorylation system. Annu. Rev. Biochem. 54, 1015-1069.
44. Kubli, D. A., and Gustafsson, Å. B. (2012) Mitochondria and mitophagy: The yin and yang of cell death control. Circ. Res. 111, 1208-1221.
45. Ye, L., Zhang, E. Y., Xiong, Q., Astle, C. M., Zhang, P., Li, Q., From, A. H., Harrison, D. E., and Zhang, J. J. (2012) Aging kit mutant mice develop cardiomyopathy. PloS ONE 7, e33407.
46. Cesselli, D., Beltrami, A. P., D'Aurizio, F., Marcon, P., Bergamin, N., Toffoletto, B., Pandolfi, M., Puppato, E., Marino, L., Signore, S., Livi, U., Verardo, R., Piazza, S., Marchionni, L., Fiorini, C., Schneider, C., Hosoda, T., Rota, M., Kajstura, J., Anversa, P., Beltrami, C. A., and Leri, A. (2011) Effects of age and heart failure on human cardiac stem cell function. Am. J. Pathol. 179, 349-366.
47. Torella, D., Rota, M., Nurzynska, D., Musso, E., Monsen, A., Shiraishi, I., Zias, E., Walsh, K., Rosenzweig, A., Sussman, M. A., Urbanek, K., Nadal-Ginard, B., Kajstura, J., Anversa, P., and Leri, A. (2004) Cardiac stem cell and myocyte aging, heart failure, and insulin-like growth factor-1 overexpression. Circ. Res. 94, 514-524.
48. Huang, C., Zhang, X., Ramil, J. M., Rikka, S., Kim, L., Lee, Y., Gude, N. A., Thistlethwaite, P. A., Sussman, M. A., Gottlieb, R. A., and Gustafsson, A. B. (2010) Juvenile exposure to anthracyclines impairs cardiac progenitor cell function and vascularization resulting in greater susceptibility to stressinduced myocardial injury in adult mice. Circulation 121, 675-683.
49. Norddahl, G. L., Pronk, C. J., Wahlestedt, M., Sten, G., Nygren, J. M., Ugale, A., Sigvardsson, M., and Bryder, D. (2011) Accumulating mitochondrial DNA mutations drive premature hematopoietic aging phenotypes distinct from physiological stem cell aging. Cell Stem Cell 8, 499-510.
50. Ahlqvist, K. J., Hämäläinen, R. H., Yatsuga, S., Uutela, M., Terzioglu, M., Götz, A., Forsström, S., Salven, P., Angers-Loustau, A., Kopra, O. H., Tyynismaa, H., Larsson, N. G., Wartiovaara, K., Prolla, T., Trifunovic, A., and Suomalainen, A. (2012) Somatic progenitor cell vulnerability to mitochondrial DNA mutagenesis underlies progeroid phenotypes in Polg mutator mice. Cell Metab. 15, 100-109.
51. Schägger, H., de Coo, R., Bauer, M. F., Hofmann, S., Godinot, C., and Brandt, U. (2004) Significance of respirasomes for the assembly/stability of human respiratory chain complex I. J. Biol. Chem. 279, 36349-36353.
52. Acín-Pérez, R., Bayona-Bafaluy, M. P., Fernández-Silva, P., Moreno- Loshuertos, R., Pérez-Martos, A., Bruno, C., Moraes, C. T., and Enríquez, J. A. (2004) Respiratory complex III is required to maintain complex I in mammalian mitochondria. Mol. Cell 13, 805-815.
53. McKenzie, M., Lazarou, M., Thorburn, D. R., and Ryan, M. T. (2006) Mitochondrial respiratory chain supercomplexes are destabilized in Barth Syndrome patients. J. Mol. Biol. 361, 462-469.
54. Kasahara, A., Cipolat, S., Chen, Y., Dorn, G. W. 2nd, and Scorrano, L. (2013) Mitochondrial fusion directs cardiomyocyte differentiation via calcineurin and Notch signaling. Science 342, 734-737.
55. Gaspar, J. A., Doss, M. X., Hengstler, J. G., Cadenas, C., Hescheler, J., and Sachinidis, A. (2014) Unique metabolic features of stem cells, cardiomyocytes, and their progenitors. Circ. Res. 114, 1346-1360.
56. Wahlestedt, M., Ameur, A., Moraghebi, R., Norddahl, G. L., Sten, G., Woods, N. B., and Bryder, D. (2014) Somatic cells with a heavy mitochondrial DNA mutational load render induced pluripotent stem cells with distinct differentiation defects. Stem Cells 32, 1173-1182.
57. Twig, G., Elorza, A., Molina, A. J., Mohamed, H., Wikstrom, J. D., Walzer, G., Stiles, L., Haigh, S. E., Katz, S., Las, G., Alroy, J., Wu, M., Py, B. F., Yuan, J., Deeney, J. T., Corkey, B. E., and Shirihai, O. S. (2008) Fission and selective fusion govern mitochondrial segregation and elimination by autophagy. EMBO J. 27, 433-446.
58. Narendra, D., Tanaka, A., Suen, D. F., and Youle, R. J. (2008) Parkin is recruited selectively to impaired mitochondria and promotes their autophagy. J. Cell Biol. 183, 795-803.
59. Andres, A. M., Stotland, A., Queliconi, B. B., and Gottlieb, R. A. (2015) A time to reap, a time to sow: Mitophagy and biogenesis in cardiac pathophysiology. J. Mol. Cell. Cardiol. 78, 62-72.
60. Hare, J. M., Fishman, J. E., Gerstenblith, G., DiFede Velazquez, D. L., Zambrano, J. P., Suncion, V. Y., Tracy, M., Ghersin, E., Johnston, P. V., Brinker, J. A., Breton, E., Davis-Sproul, J., Schulman, I. H., Byrnes, J., Mendizabal, A. M., Lowery, M. H., Rouy, D., Altman, P., Wong Po Foo, C., Ruiz, P., Amador, A., Da Silva, J., McNiece, I. K., Heldman, A. W., George, R., and Lardo, A. (2012) Comparison of allogeneic vs autologous bone marrow- derived mesenchymal stem cells delivered by transendocardial injection in patients with ischemic cardiomyopathy: The POSEIDON randomized trial. JAMA 308, 2369-2379.
61. Heldman, A. W., DiFede, D. L., Fishman, J. E., Zambrano, J. P., Trachtenberg, B. H., Karantalis, V., Mushtaq, M., Williams, A. R., Suncion, V. Y., McNiece, I. K., Ghersin, E., Soto, V., Lopera, G., Miki, R., Willens, H., Hendel, R., Mitrani, R., Pattany, P., Feigenbaum, G., Oskouei, B., Byrnes, J., Lowery, M. H., Sierra, J., Pujol, M. V., Delgado, C., Gonzalez, P. J., Rodriguez, J. E., Bagno, L. L., Rouy, D., Altman, P., Foo, C. W., da Silva, J., Anderson, E., Schwarz, R., Mendizabal, A., and Hare, J. M. (2014) Transendocardial mesenchymal stem cells and mononuclear bone marrow cells for ischemic cardiomyopathy: The TAC-HFT randomized trial. JAMA 311, 62-73.
62. Karantalis, V., DiFede, D. L., Gerstenblith, G., Pham, S., Symes, J., Zambrano, J. P., Fishman, J., Pattany, P., McNiece, I., Conte, J., Schulman, S., Wu, K., Shah, A., Breton, E., Davis-Sproul, J., Schwarz, R., Feigenbaum, G., Mushtaq, M., Suncion, V. Y., Lardo, A. C., Borrello, I., Mendizabal, A., Karas, T. Z., Byrnes, J., Lowery, M., Heldman, A. W., and Hare, J. M. (2014) Autologous mesenchymal stem cells produce concordant improvements in regional function, tissue perfusion, and fibrotic burden when administered to patients undergoing coronary artery bypass grafting: The Prospective Randomized Study of Mesenchymal Stem Cell Therapy in Patients Undergoing Cardiac Surgery (PROMETHEUS) trial. Circ. Res. 114, 1302-1310.