Single-Cell Whole-Genome Amplification and Sequencing: Methodology and Applications

Annual Review of Genomics and Human Genetics

Volumn 16, Issue , 2015, Pages 79-102

  Huang, Lei ^a   Ma, Fei ^a   Chapman, Alec ^b,c   Lu, Sijia ^d   Xie, Xiaoliang Sunney ^a,b  

^a PEKING UNIVERSITY   (China)

^b Department of Chemistry and Chemical Biology ^*  (China)

^c HARVARD UNIVERSITY   (United States)

^d Yikon Genomics Co Ltd   (China)

Author keywords

Cancer genomics; CTC; DOP PCR; In vitro fertilization; MALBAC; MDA; PGD; PGS; Single cell genomics; WGA

Indexed keywords

DNA POLYMERASE; OLIGONUCLEOTIDE;

ALLELE; ARTICLE; CANCER DIAGNOSIS; CHIMERA; CIRCULATING TUMOR CELL; COPY NUMBER VARIATION; CYTOMETRY; DNA SEQUENCE; FALSE POSITIVE RESULT; FERTILIZATION IN VITRO; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC SCREENING; GENETIC VARIABILITY; GENOME ANALYSIS; GENOMIC INSTABILITY; GENOMICS; GERM CELL; HEALTH SURVEY; HUMAN; HUMAN GENETICS; HUMAN GENOME; INTERMETHOD COMPARISON; MEIOSIS; MEIOTIC RECOMBINATION; MOLECULAR DIAGNOSIS; MOLECULAR EVOLUTION; MULTIPLE ANNEALING AND LOOPING BASED AMPLIFICATION CYCLE; MULTIPLE DISPLACEMENT AMPLIFICATION; MUTATION; MUTATION RATE; NEOPLASM; NEXT GENERATION SEQUENCING; OLIGONUCLEOTIDE PRIMED POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION; PREIMPLANTATION EMBRYO; PREIMPLANTATION GENETIC DIAGNOSIS; PREIMPLANTATION GENOMIC SCREENING; REPRODUCIBILITY; REPRODUCTION; SINGLE CELL ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; TRANSCRIPTOMICS; WHOLE GENOME AMPLIFICATION; FEMALE; GENETICS; HIGH THROUGHPUT SEQUENCING; MALE; PHYSIOLOGY; PREGNANCY; PRENATAL DIAGNOSIS; PROCEDURES;

FEMALE; FERTILIZATION IN VITRO; GENOME, HUMAN; GENOMICS; GERM CELLS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MALE; MUTATION RATE; NEOPLASMS; POLYMERASE CHAIN REACTION; PREGNANCY; PREIMPLANTATION DIAGNOSIS; SINGLE-CELL ANALYSIS;

EID: 84940644487     PISSN: 15278204     EISSN: 1545293X     Source Type: Book Series    
DOI: 10.1146/annurev-genom-090413-025352     Document Type: Article

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