The Structure and Enzyme Characteristics of a Recombinant Leucine Aminopeptidase rLap1 from Aspergillus sojae and Its Application in Debittering

Applied Biochemistry and Biotechnology

Volumn 177, Issue 1, 2015, Pages 190-206

  Huang, Wei Qian ^a   Zhong, Li Fen ^a   Meng, Zhi Zhong ^a   You, Zi Juan ^a   Li, Jia Zhou ^b   Luo, Xiao Chun ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b Department of Environmental Engineering   (China)

Author keywords

3D structure; Aspergillus sojae; Debittering; Pichia pastoris; Recombinant leucine aminopeptidase

Indexed keywords

ASPERGILLUS; CLONING; COMPLEX NETWORKS; HYDROGEN BONDS; HYDROLYSIS; MANGANESE; SUBSTRATES;

3D STRUCTURE; DEBITTERING; DEGREE OF HYDROLYSIS; ENZYME CHARACTERISTICS; HYDROGEN BOND NETWORKS; LEUCINE AMINOPEPTIDASE; PICHIA PASTORIS; SOY PROTEIN HYDROLYSATE;

AMINO ACIDS;

AMINO ACID; ARGININE; CASEIN; COMPLEMENTARY DNA; CYTOSOL AMINOPEPTIDASE; FUNGAL ENZYME; FUNGAL RNA; LEUCINE; LYSINE; METHIONINE; PHENYLALANINE; PROTEIN HYDROLYSATE; RECOMBINANT ENZYME; RECOMBINANT LEUCINE AMINOPEPTIDASE RLAP1; SOYBEAN PROTEIN; UNCLASSIFIED DRUG; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; ASPERGILLUS PARASITICUS; BITTER TASTE; CATALYSIS; CLONING; DEBITTERING; DIPOLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; EXPRESSION VECTOR; FED BATCH FERMENTATION; FOOD INDUSTRY; FOOD PROCESSING; HYDROGEN BOND; KOMAGATAELLA PASTORIS; MOLECULAR DOCKING; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PROTEIN HYDROLYSIS; THERMOSTABILITY; AMINO ACID SEQUENCE; ASPERGILLUS; CHEMICAL PHENOMENA; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENE VECTOR; HYDROLYSIS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ALIGNMENT; STRUCTURAL HOMOLOGY; TASTE; TEMPERATURE;

ASPERGILLUS SOJAE; PICHIA PASTORIS;

AMINO ACID SEQUENCE; ASPERGILLUS; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; GENETIC VECTORS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LEUCYL AMINOPEPTIDASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; STRUCTURAL HOMOLOGY, PROTEIN; SUBSTRATE SPECIFICITY; TASTE; TEMPERATURE;

EID: 84940451383     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-015-1737-5     Document Type: Article

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