Kinetic, spectroscopic, and X-ray crystallographic characterization of the functional E151H aminopeptidase from Aeromonas proteolytica

Biochemistry

Volumn 44, Issue 36, 2005, Pages 12030-12040

  Bzymek, Krzysztof P ^a   Moulin, Aaron ^b   Swierczek, Sabina I ^a   Ringe, Dagmar ^a,b   Petsko, Gregory A ^a,b   Bennett, Brian ^a,c   Holz, Richard C ^a  

^a UTAH STATE UNIVERSITY   (United States)

^b BRANDEIS UNIVERSITY   (United States)

^c MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYMES; HYDROLYSIS; PROTEINS; PROTON BEAMS; X RAY CRYSTALLOGRAPHY;

AMINOPEPTIDASE FROM VIBRIO PROTEOLYTICUS (AAP); ENZYME-SUBSTRATE COMPLEX; HISTIDONE; KINETIC PARAMETERS;

POLYPEPTIDES;

ACID; AMINOPEPTIDASE; ANILIDE; BASE; COBALT; GLUTAMIC ACID; HISTIDINE; HYDROLASE; LEUCINE 4 NITROANILIDE; METALLOHYDROLASE; UNCLASSIFIED DRUG; ZINC;

AEROMONAS; AEROMONAS PROTEOLYTICA; ALKALINITY; AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ELECTRON SPIN RESONANCE; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; IONIZATION; ISOTOPE LABELING; NONHUMAN; PARAMETER; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; PROTON TRANSPORT; SPECTROPHOTOMETRY; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

AEROMONAS HYDROPHILA; AMINOPEPTIDASES; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; KINETICS; MUTATION; PROTEIN STRUCTURE, TERTIARY; SPECTRUM ANALYSIS; TEMPERATURE; TITRIMETRY;

BACILLUS SUBTILIS; VIBRIO PROTEOLYTICUS;

EID: 24644471886     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0505823     Document Type: Article

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