Biochemical properties and potential applications of recombinant leucine aminopeptidase from bacillus kaustophilus CCRC 11223

International Journal of Molecular Sciences

Volumn 12, Issue 11, 2011, Pages 7609-7625

  Shen, Yanfei ^a   Wang, Fanghua ^a   Lan, Dongming ^a   Liu, Yuanyuan ^a   Yang, Bo ^a   Wang, Yonghua ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Anchovy; Bacillus kaustophilus CCRC 11223; Biochemical properties; Circular dichroism spectroscopy; Hydrolysis; Leucine aminopeptidase

Indexed keywords

ANCHOVY PROTEIN; BARIUM ION; BUFFER; CALCIUM ION; CITRATE PHOSPHATE BUFFER; COBALT; COPPER ION; CYTOSOL AMINOPEPTIDASE; FERRIC ION; FERROUS ION; FISH PROTEIN; GLYCINE SODIUM HYDROXIDE BUFFER; HEPES BUFFER; LITHIUM ION; MAGNESIUM ION; MANGANESE; MCILVANIE BUFFER; MERCURY; NICKEL; POTASSIUM DIHYDROGEN PHOSPHATE; RECOMBINANT ENZYME; RECOMBINANT LEUCINE AMINOPEPTIDASE; TRIS HYDROCHLORIC ACID BUFFER; UNCLASSIFIED DRUG; ZINC ION; AMINO ACID; PROTEIN HYDROLYSATE; RECOMBINANT PROTEIN;

ALPHA HELIX; AMINO ACID COMPOSITION; ARTICLE; BACILLUS; BACILLUS KAUSTOPHILUS; BETA SHEET; CIRCULAR DICHROISM; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME PURIFICATION; FOOD PROCESSING; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; PROTEIN SECONDARY STRUCTURE; PROTEIN UNFOLDING; TEMPERATURE SENSITIVITY; ANIMAL; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; FISH; GENETICS; HEAT; HYDROLYSIS; METABOLISM; PLASMID;

AMINO ACIDS; ANIMALS; BACILLUS; FISH PROTEINS; FISHES; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; LEUCYL AMINOPEPTIDASE; MOLECULAR WEIGHT; PLASMIDS; PROTEIN HYDROLYSATES; RECOMBINANT PROTEINS;

EID: 82255185797     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12117609     Document Type: Article

References (29)

1. Eun, J.S.; Beauchemin, K.A.; Hong, S.H.; Bauer, M.W. Exogenous enzymes added to untreated or ammoniated rice straw: Effects on in vitro fermentation characteristics and degradability. Anim. Feed Sci. Technol. 2006, 131, 87-102.
2. EIBoushy, A.R.; van der Poel, A.F.B.; Walraven, O.E.D. Feather meal-A biological waste: Its processing and utilization as a feedstuff for poultry. Biol. Wastes 1990, 32, 39-74.
3. Nodar, R.; Acea, M.J.; Carballas, T. Microbial compostion of poultry excreta. Biol. Wastes 1990, 33, 95-105.
4. Urlings, H.A.; van Logtestijn, J.G.; Bijker, P.G. Slaughter by-products: Problems, preliminary research and possible solutions. Vet. Quart. 1992, 14, 34-38.
5. Urlings, H.A.; Fransen, N.G.; Bijker, P.G.; van Logtestijn, J.G. Proteolysis and amino acid breakdown of heated and irradiated poultry byproducts and muscle tissue. J. Anim. Sci. 1993, 71, 2432-2438.
6. Yu, J.; Ahmedna, M.; Goktepe, I. Peanut protein concentrate: Production and functional properties as affected by processing. Food Chem. 2007, 103, 121-129.
7. Guerard, F.; Dufosse, L.; Broise, D.D.L.; Binet, A. Enzymatic hydrolysis of proteins from yellowfin tuna (Thunnus albacares) wastes using Alcalase. J. Mol. Catal. B Enzym. 2001, 11, 1051-1059.
8. Quist, E.E.; Phillips, R.D.; Saalia, F.K. The effect of enzyme systems and processing on the hydrolysis of peanut (Arachis hypogaea L.) protein. LWT Food Sci. Technol. 2009, 42, 1717-1721.
9. Saha, B.C.; Hayashi, K. Debittering of protein hydrolyzates. Biotechnol. Adv. 2001, 19, 355-370.
10. Kim, H.; Lipscomb, W.N. Structure and mechanism of bovine lens leucine aminopeptidase. Adv. Enzymol. Areas Mol. Biol. 1994, 68, 153-213.
11. Rao, M.B.; Tanksale, A.M.; Ghatge, M.S.; Deshpande, V.V. Molecular and biotechnological aspects of microbial protease. Microbiol. Mol. Biol. Rev. 1998, 62, 597-635.
12. Kamphuis, J.; Meijer, E.M.; Boesten, W.H.J.; Broxterman, Q.B.; Kaptein, B.; Hermes, H.F.M.; Schoemaker, H.E. Production of natural and synthetic L- and D-amino acids by aminopeptidases and amino amidases. In Biocatalytic Production of Amino Acids and Derivatives; Rozzell, J.D., Wagner, F., Eds.; Wiley: New York, NY, USA, 1992; pp. 178-206.
13. Toldra, F.; Aristoy, A.C.; Flores, M. Contribution of muscle aminopeptidase to flavor development in dry cured ham. Food Res. Int. 2000, 33, 181-185.
14. Lin, L.L.; Hsu, W.H.; Wu, C.P.; Chi, M.C.; Chou, W.M.; Hu, H.Y. A thermostable leucine aminopeptidase from Bacillus kaustophilus CCRC 11223. Extremophiles 2004, 8, 79-87.
15. Chi, M.C.; Huang, H.B.; Liu, J.S.; Wang, W.C.; Liang, W.C.; Lin, L.L. Residues threonine346 and leucine 352 are critical for the proper function of Bacillus kaustophilus leucine aminopeptidase. FEMS Microbiol. Lett. 2006, 260, 156-161.
16. Chi, M.C.; Liu, J.S.; Wang, W.C.; Lin, L.L.; Huang, H.B. Site-directed mutagenesis of the conserved Ala348 and Gly350 residues at the putative active site of Bacillus kaustophilus aminopeptidase. Biochimie 2008, 90, 811-819.
17. Magboul, A.A.A.; McSweeney, P.L.H. Purification and characterization of an aminopeptidase from Lactobacillus curvatus DPC2024. Int. Dairy J. 1999, 9, 107-116.
18. Montel, M.C.; Seronie, M.P.; Talon, R.; Hébraud, M. Purification and characterization of a dipeptidase from Lactobacillus sake. Appl. Environ. Microbiol. 1995, 61, 837-839.
19. Ramachandrin, L.K.; Witkop, B. The interaction of mercuric acetate with indoles, tryptophan and proteins. Biochemistry 1964, 3, 1603-1611.
20. Xia, S.Y.; Deng, S.G.; Xie, C.; Huo, J.C. Nutritional ingredient and antihyperlipidemia action of protein hyrolysates from anchovy Engraulis japonicus. Oceanologia Et Limnologia Sini. 2008, 39, 368-373.
21. Siringan, P.; Raksakulthai, I.; Yongsawatdigul, J. Partial purification and characterization of trypsin-like proteinases in Indian anchovy. Food Chem. 2007, 101, 82-89.
22. Pedroche, J.; Yust, M.M.; Girón-Calle, J.; Alaiz, M.; Millán, F.; Vioque, J. Utilization of chickpea protein isolates for production of peptides with angiotensin I-converting enzyme (ACE)-inhibitory activity. J. Sci. Food Agric. 2002, 82, 960-965.
23. Plumb, G.W.; Mills, E.N.C.; Tatton, M.J.; Dursel, C.C.M.; Lambert, N.; Morgan, M.R.A. Effect of thermal and proteolytic processing on glycinin, the 11S globulin of soy (Glycine max): A study utilizing monoclonal and polyclonal antibodies. J. Agric. Food Chem. 1994, 42, 834-840.
24. Chiang, W.D.; Shih, C.J.; Chu, Y.H. Functional properties of soy protein hydrolysate produced from a continuous membrane reactor system. Food Chem. 1999, 65, 189-194.
25. Xiong, A.S.; Yao, Q.H.; Peng, R.H.; Li, X.; Fan, H.Q.; Cheng, Z.M.; Li, Y. A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences. Nucl. Acids Res. 2004, 32, doi: 10.1093/nar/gnh094.
26. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970, 227, 680-685.
27. Kuo, L.Y.; Hwang, G.Y.; Lai, Y.J.; Yang, S.L.; Lin, L.L. Overexpression, purification, and characterization of the recombinant leucine aminopeptidase II of Bacillus stearothermophilus. Curr. Microbiol. 2003, 47, 40-45.
28. Nilsang, S.; Lertsiri, S.; Suphantharika, M.; Assavanig, A.; Optimization of enzymatic hydrolysis of fish soluble concentrate by commercial proteases. J. Food Eng. 2005, 70, 571-578.
29. Liu, C.; Wang, H.L.; Cui, Z.M.; He, X.L.; Wang, X.S.; Zeng, X.X.; Ma, H. Optimization of extraction and isolation for 11S and 7S globulins of soybean seed storage protein. Food Chem. 2007, 102, 1310-1316.