Enhanced protein production by microRNA-30 family in CHO cells is mediated by the modulation of the ubiquitin pathway

Journal of Biotechnology

Volumn 212, Issue , 2015, Pages 32-43

  Fischer, Simon ^a,b,h   Mathias, Sven ^a,b   Schaz, Simone ^a,b   Emmerling, Verena Vanessa ^b,c   Buck, Theresa ^a   Kleemann, Michael ^a   Hackl, Matthias ^d   Grillari, Johannes ^e,f   Aschrafi, Armaz ^g   Handrick, René ^a   Otte, Kerstin ^a  

^a BIBERACH UNIVERSITY OF APPLIED SCIENCES   (Germany)

^b UNIVERSITY OF ULM   (Germany)

^c Rentschler Biotechnologie GmbH   (Germany)

^d TAmiRNA GmbH   (Austria)

^e UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^f Evercyte GmbH   (Austria)

^g RADBOUD UNIVERSITY NIJMEGEN   (Netherlands)

^h Boehringer Ingelheim GmbH and Co KG   (Germany)

Author keywords

Cell engineering; CHO; MicroRNA; MiR 30; Skp2; Ubiquitin pathway

Indexed keywords

CELL CULTURE; GENES; MODULATION; RECOMBINANT PROTEINS; RNA;

CHINESE HAMSTER OVARY CELLS; FUNCTIONAL VALIDATION; MICRORNAS; MIR-30; RECOMBINANT PROTEIN EXPRESSION; RECOMBINANT PROTEIN PRODUCTIONS; SKP2; UBIQUITIN;

CELL ENGINEERING;

MICRORNA; MICRORNA 30; RECOMBINANT PROTEIN; S PHASE KINASE ASSOCIATED PROTEIN 2; UBIQUITIN; UNCLASSIFIED DRUG; MESSENGER RNA; S PHASE KINASE ASSOCIATED PROTEIN; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; CHO CELL LINE; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; GENE TARGETING; GENETIC REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; ANIMAL; CRICETULUS; GENETICS; HAMSTER; HEK293 CELL LINE; HUMAN; METABOLISM; SIGNAL TRANSDUCTION;

CRICETULUS GRISEUS;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; HEK293 CELLS; HUMANS; MICRORNAS; RECOMBINANT PROTEINS; RNA, MESSENGER; RNA, SMALL INTERFERING; S-PHASE KINASE-ASSOCIATED PROTEINS; SIGNAL TRANSDUCTION; UBIQUITIN;

EID: 84940023437     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2015.08.002     Document Type: Article

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