Engineering Yarrowia lipolytica for production of medium-chain fatty acids

Applied Microbiology and Biotechnology

Volumn 99, Issue 17, 2015, Pages 7359-7368

  Rutter, Charles D ^a   Zhang, Shuyan ^a   Rao, Christopher V ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Acyl ACP thioesterase; Medium chain fatty acids; Metabolic engineering; Oleaginous yeast

Indexed keywords

CHAINS; LIPIDS; METABOLIC ENGINEERING; YEAST;

ACYL CARRIER PROTEINS; LIPID PRODUCTIVITIES; LONG CHAIN FATTY ACID; MEDIUM-CHAIN FATTY ACIDS; OLEAGINOUS YEASTS; PHYSICAL AND CHEMICAL PROPERTIES; THIOESTERASES; YARROWIA LIPOLYTICA;

FATTY ACIDS;

ACYL CARRIER PROTEIN; DECANOIC ACID; MEDIUM CHAIN FATTY ACID; OCTANOIC ACID; THIOL ESTER HYDROLASE; DECANOIC ACID DERIVATIVE; FATTY ACID; OCTANOIC ACID DERIVATIVE; PALMITOYL COENZYME A HYDROLASE; RECOMBINANT PROTEIN;

BIOENGINEERING; FATTY ACID; METABOLISM; MOLECULAR ANALYSIS; PETROLEUM HYDROCARBON; PHYSICOCHEMICAL PROPERTY; PROTEIN; YEAST;

ARTICLE; ENZYME ACTIVITY; FATTY ACID SYNTHESIS; LIPID ANALYSIS; METABOLIC ENGINEERING; PLASMID; PROTEIN EXPRESSION; YARROWIA LIPOLYTICA; BIOSYNTHESIS; GENE EXPRESSION; GENETICS; METABOLISM; YARROWIA;

YARROWIA LIPOLYTICA;

ACYL CARRIER PROTEIN; BIOSYNTHETIC PATHWAYS; CAPRYLATES; DECANOIC ACIDS; FATTY ACIDS; GENE EXPRESSION; METABOLIC ENGINEERING; PALMITOYL-COA HYDROLASE; RECOMBINANT PROTEINS; YARROWIA;

EID: 84939271662     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6764-1     Document Type: Article

References (58)

1. Ageitos JM, Vallejo JA, Veiga-Crespo P, Villa TG (2011) Oily yeasts as oleaginous cell factories. Appl Microbiol Biotechnol 90(4):1219–1227
2. Beopoulos A, Chardot T, Nicaud JM (2009) Yarrowia lipolytica: a model and a tool to understand the mechanisms implicated in lipid accumulation. Biochimie 91:692–696
3. Beopoulos A, Verbeke J, Bordes F, Guicherd M, Bressy M, Marty A, Nicaud JM (2014) Metabolic engineering for ricinoleic acid production in the oleaginous yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 98(1):251–262
4. Blazeck J, Liu L, Knight R, Alper HS (2013) Heterologous production of pentane in the oleaginous yeast Yarrowia lipolytica. J Biotechnol 165(3):184–194
5. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917
6. Carroll KK (1961) Separation of lipid classes by chromatography on Florisil. J Lipid Res 2(2):135–141
7. Chandler DS, Qi J, Mattox W (2003) Direct repression of Splicing by transformer-2. Mol Cell Biol 23(15):5174–5185
8. Chen L, Zhang J, Chen WN (2014) Engineering the Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel. PLoS One 9(1)
9. Choi YJ, Lee SY (2013) Microbial production of short-chain alkanes. Nature 502(7472):571–574
10. Cronan JE Jr, Rock CO (1996) Biosynthesis of membrane lipids Escherichia coli and Salmonella: cellular and molecular biology, 2nd edn. ASM Press, Washington, DC, pp 612–636
11. Dörmann P, Spener F, Ohlrogge JB (1993) Characterization of two acyl-acyl carrier protein thioesterases from developing Cuphea seeds specific for medium-chain-and oleoyl-acyl carrier protein. Planta 189(3):425–432
12. Dwivedi G, Sharma M (2014) Impact of cold flow properties of biodiesel on engine performance. Renew Sustain Energy Rev 31:650–656
13. Eccleston VS, Cranmer AM, Voelker TA, Ohlrogge JB (1996) Medium-chain fatty acid biosynthesis and utilization in Brassica napus plants expressing lauroyl-acyl carrier protein thioesterase. Planta 198(1):46–53
14. Fortman J, Chhabra S, Mukhopadhyay A, Chou H, Lee TS, Steen E, Keasling JD (2008) Biofuel alternatives to ethanol: pumping the microbial well. Trends Biotechnol 26(7):375–381
15. Freeman C, West D (1966) Complete separation of lipid classes on a single thin-layer plate. J Lipid Res 7(2):324–327
16. Gietz RD, Woods RA (2002) Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol 350:87–96
17. Harwood JL (1988) Fatty acid metabolism. Annu Rev Plant Physiol Plant Mol Biol 39(1):101–138
18. Jiang P, Cronan J (1994) Inhibition of fatty acid synthesis in Escherichia coli in the absence of phospholipid synthesis and release of inhibition by thioesterase action. J Bacteriol 176(10):2814–2821
19. Jing F, Cantu DC, Tvaruzkova J, Chipman JP, Nikolau BJ, Yandeau-Nelson MD, Reilly PJ (2011) Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity. BMC Biochem 12(1):44
20. Landriscina C, Gnoni GV, Quagliariello E (1972) Fatty‐acid biosynthesis. Eur J Biochem 29(1):188–196
21. Leber C, Da Silva N (2014) Engineering of Saccharomyces cerevisiae for the synthesis of short chain fatty acids. Biotechnol Bioeng 111(2):347–358
22. Leibundgut M, Maier T, Jenni S, Ban N (2008) The multienzyme architecture of eukaryotic fatty acid synthases. Curr Opin Struct Biol 18:714–725
23. Lepage G, Roy CC (1984) Improved recovery of fatty acid through direct transesterification without prior extraction or purification. J Lipid Res 25(12):1391–1396
24. Liu T, Vora H, Khosla C (2010) Quantitative analysis and engineering of fatty acid biosynthesis in E. coli. Metab Eng 12(4):378–386
25. Liu H, Cheng T, Xian M, Cao Y, Fang F, Zou H (2014) Fatty acid from the renewable sources: a promising feedstock for the production of biofuels and biobased chemicals. Biotechnol Adv 32(2):382–389
26. Liu L, Pan A, Spofford C, Zhou N, Alper HS (2015) An evolutionary metabolic engineering approach for enhancing lipogenesis in Yarrowia lipolytica. Metab Eng 29:36–45
27. Lomakin IB, Xiong Y, Steitz TA (2007) The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together. Cell 129(2):319–332
28. Lu X, Vora H, Khosla C (2008) Overproduction of free fatty acids in E. coli: implications for biodiesel production. Metab Eng 10(6):333–339
29. Lynd LR, van Zyl WH, McBride JE, Laser M (2005) Consolidated bioprocessing of cellulosic biomass: an update. Curr Opin Biotechnol 16(5):577–583
30. Lynen F (1969) Yeast fatty acid synthase. Methods Enzymol 14:17–33
31. Madzak C (2015) Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering. Appl Microbiol Biotechnol 99(11):4559–4577
32. Madzak C, Tréton B, Blanchin-Roland S (2000) Strong hybrid promoters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolytica. J Mol Microbiol Biotechnol 2(2):207–216
33. McMahon MD, Prather KL (2014) Functional screening and in vitro analysis reveal thioesterases with enhanced substrate specificity profiles that improve short-chain fatty acid production in Escherichia coli. Appl Environ Microbiol 80:1042–1050
34. Moreno-Pérez AJ, Venegas-Caleron M, Vaistij FE, Salas JJ, Larson TJ, Garces R, Graham IA, Martinez-Force E (2012) Reduced expression of FatA thioesterases in Arabidopsis affects the oil content and fatty acid composition of the seeds. Planta 235(3):629–639
35. Moser BR (2011) Biodiesel production, properties, and feedstocks. In: Biofuels. Springer, pp 285–347
36. Ohlrogge JB, Jaworski JG (1997) Regulation of fatty acid synthesis. Annu Rev Plant Biol 48:109–136
37. Papanikolaou S, Aggelis G (2011) Lipids of oleaginous yeasts. Part II: technology and potential applications. Eur J Lipid Sci Technol 113(8):1052–1073
38. Pollard MR, Anderson L, Fan C, Hawkins DJ, Davies HM (1991) A specific acyl-ACP thioesterase implicated in medium-chain fatty acid production in immature cotyledons of Umbellularia californica. Arch Biochem Biophys 284(2):306–312
39. Qiao K, Abidi SHI, Liu H, Zhang H, Chakraborty S, Watson N, Stephanopoulos G (2015) Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica. Metab Eng 29:56–65
40. Ramos MJ, Fernández CM, Casas A, Rodríguez L, Pérez Á (2009) Influence of fatty acid composition of raw materials on biodiesel properties. Bioresour Technol 100(1):261–268
41. Runguphan W, Keasling JD (2014) Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals. Metab Eng 21:103–113
42. Santos TM, Pinto A, Oliveira AV, Ribeiro HL, Caceres CA, Ito EN, Azeredo H (2014) Physical properties of cassava starch–carnauba wax emulsion films as affected by component proportions. Int J Food Sci Technol 49(9):2045–2051
43. Sherkhanov S, Korman TP, Bowie JU (2014) Improving the tolerance of Escherichia coli to medium-chain fatty acid production. Metab Eng 25:1–7
44. Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Keasling JD (2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature 463(7280):559–562
45. Tai M, Stephanopoulos G (2013) Engineering the push and pull of lipid biosynthesis in oleaginous yeas Yarrowia lipolytica for biofuel production. Metab Eng 15:1–9
46. Tariq M, Ali S, Ahmad F, Ahmad M, Zafar M, Khalid N, Khan MA (2011) Identification, FT-IR, NMR (1 H and 13 C) and GC/MS studies of fatty acid methyl esters in biodiesel from rocket seed oil. Fuel Process Technol 92(3):336–341
47. Viegas CA, Rosa MF, Sá-Correia I, Novais JM (1989) Inhibition of yeast growth by octanoic and decanoic acids produced during ethanolic fermentation. Appl Environ Microbiol 55(1):21–28
48. Voelker TA, Worrell AC, Anderson L, Bleibaum J, Fan C, Hawkins DJ, Davies HM (1992) Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257(5066):72–74
49. Voelker TA, Jones A, Cranmer AM, Davies HM, Knutzon DS (1997) Broad-range and binary-range acyl-acyl-carrier-protein thioesterases suggest an alternative mechanism for medium-chain production in seeds. Plant Physiol 114(2):669–677
50. von Wettstein‐Knowles P, Olsen JG, McGuire KA, Henriksen A (2006) Fatty acid synthesis. FEBS J 273(4):695–710
51. Wakil SJ (1989) Fatty acid synthase, a proficient multifunctional enzyme. Biochemistry 28(11):4523–4530
52. Wang G-Y, Zhang Y, Chi Z, Liu G-L, Wang Z-P, Chi Z-M (2014) Role of pyruvate carboxylase in accumulation of intracellular lipid of the oleaginous yeast Yarrowia lipolytica ACA-DC 50109. Appl Microbiol Biotechnol 1–9
53. White SW, Zheng J, Zhang YM, Rock CO (2005) The structural biology of type II fatty acid biosynthesis. Annu Rev Biochem 74:791–831
54. Xue Z, Sharpe PL, Hong SP, Yadav NS, Xie D, Short DR, Damude HG, Ruper RA, Seip JE, Wang J, Pollak DW, Bostick MW, Bosak MD, Macool DJ, Hollerbach DH, Zhang H, Arcilla DM, Bledsoe SA, Croker K, McCord EF, Tyreus BD, Jackson EN, Zhu Q (2013) Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nat Biotechnol 31(8):734–740
55. Zhang YM, White SW, Rock CO (2006) Inhibiting bacterial fatty acid synthesis. J Biol Chem 281(26):17541–17544
56. Zhang B, Chen H, Li M, Gu Z, Song Y, Ratledge C, Chen W (2013) Genetic engineering of Yarrowia lipolytica for enhanced production of trans-10, cis-12 conjugated linoleic acid. Microb Cell Factories 12(1):70
57. Zhang H, Zhang L, Chen H, Chen YQ, Chen W, Song Y, Ratledge C (2014) Enhanced lipid accumulation in the yeast Yarrowia lipolytica by over-expression of ATP: citrate lyase from Mus musculus. J Biotechnol 192:78–84
58. Zheng Y-N, Li L-L, Liu Q, Yang J-M, Wang Z-W, Liu W, Xu X, Liu H, Zhao G, Xian M (2012) Optimization of fatty alcohol biosynthesis pathway for selectively enhanced production of C12/14 and C16/18 fatty alcohols in engineered Escherichia coli. Microb Cell Factories 11:65