CRISPR-Cas9-Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library

Cell Stem Cell

Volumn 17, Issue 2, 2015, Pages 221-232

  Zhong, Cuiqing ^a,b   Yin, Qi ^a,b   Xie, Zhenfei ^a,b   Bai, Meizhu ^a,c   Dong, Rui ^b,d   Tang, Wei ^a   Xing, Yu Hang ^a,b   Zhang, Hongling ^a,c   Yang, Suming ^a,b   Chen, Ling Ling ^a,b   Bartolomei, Marisa S ^e   Ferguson Smith, Anne ^f   Li, Dangsheng ^a,g   Yang, Li ^d   Wu, Yuxuan ^a   Li, Jinsong ^a,c  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c SHANGHAITECH UNIVERSITY   (China)

^d MAX PLANCK INSTITUTE   (Germany)

^e UNIVERSITY OF PENNSYLVANIA   (United States)

^f UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^g Shanghai Jiao Tong University Affiliated Sixth People s Hospital   (China)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN; RNA; TET1 PROTEIN; TET2 PROTEIN; TET3 PROTEIN; UNCLASSIFIED DRUG; GUIDE RNA;

ALLELE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL NUCLEUS TRANSPLANTATION; CHROMOSOME 12; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; DNA MODIFICATION; DNA SEQUENCE; ECFP GENE; EMBRYO; EMBRYONIC STEM CELL; FEMALE; FLOW CYTOMETRY; GENE; GENE DELETION; GENE DISRUPTION; GENE EXPRESSION; GENE INSERTION; GENE MUTATION; GENE REPRESSION; GENETIC ANALYSIS; GENETIC MANIPULATION; GENETIC SCREENING; GENETIC TRANSFECTION; GENOTYPE; GTL2 GENE; H19 GENE; HAPLOIDY; IN VITRO STUDY; INDEL MUTATION; INHERITANCE; MALE; MOLECULAR CLONING; MOUSE; MOUSE MUTANT; MUTAGEN TESTING; MUTAGENESIS; MUTATION RATE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROGENY; RNA LIBRARY; RNA SEQUENCE; RNA STRUCTURE; SPERMATOZOON HEAD; ANIMAL; C57BL MOUSE; CLONING; DNA METHYLATION; GENE LIBRARY; GENETICS; HETEROZYGOTE; KNOCKOUT MOUSE; METABOLISM; MOLECULAR GENETICS; MOUSE EMBRYONIC STEM CELL; MUTANT MOUSE STRAIN; NUCLEOTIDE SEQUENCE;

MUS;

ALLELES; ANIMALS; BASE SEQUENCE; CLONING, ORGANISM; CRISPR-CAS SYSTEMS; DNA METHYLATION; GENE LIBRARY; GENETIC TESTING; HAPLOIDY; HETEROZYGOTE; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; MICE, MUTANT STRAINS; MOLECULAR SEQUENCE DATA; MOUSE EMBRYONIC STEM CELLS; RNA, GUIDE;

EID: 84938746604     PISSN: 19345909     EISSN: 18759777     Source Type: Journal    
DOI: 10.1016/j.stem.2015.06.005     Document Type: Article

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