Transfection of brain capillary endothelial cells in primary culture with defined blood-brain barrier properties

Fluids and Barriers of the CNS

Volumn 12, Issue 1, 2015, Pages

  Burkhart, Annette ^a   Thomsen, Louiza Bohn ^a   Thomsen, Maj Schneider ^a   Lichota, Jacek ^a   Fazakas, Csilla ^b   Krizbai, István ^b   Moos, Torben ^a  

^a AALBORG UNIVERSITY   (Denmark)

^b INSTITUTE OF BIOCHEMISTRY   (Hungary)

Author keywords

Astrocyte; Blood brain barrier; Endothelium; Gene therapy; In vitro; Pericytes; Primary culture; Transfection

Indexed keywords

BRAIN PROTEIN; CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; MANNITOL; POLYMER; PROTEIN HCRED1 C1; RED FLUORESCENT PROTEIN; TIGHT JUNCTION PROTEIN; TURBOFECT; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ASTROCYTE; BLOOD BRAIN BARRIER; BRAIN CAPILLARY ENDOTHELIAL CELL; CELL MEMBRANE PERMEABILITY; COCULTURE; ELECTRIC RESISTANCE; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; GENETIC TRANSFECTION; IN VITRO STUDY; MITOSIS; NEWBORN; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PERICYTE; PRIMARY CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RAT; ANIMAL; CAPILLARY PERMEABILITY; CELL CULTURE; CELL CULTURE TECHNIQUE; CELL DIVISION; CYTOLOGY; ENDOTHELIUM CELL; METABOLISM; PHYSIOLOGY; PROCEDURES; SPRAGUE DAWLEY RAT;

ANIMALS; ASTROCYTES; BLOOD-BRAIN BARRIER; CAPILLARY PERMEABILITY; CELL CULTURE TECHNIQUES; CELL DIVISION; CELLS, CULTURED; COCULTURE TECHNIQUES; ENDOTHELIAL CELLS; PERICYTES; RATS; RATS, SPRAGUE-DAWLEY; TRANSFECTION;

EID: 84938570940     PISSN: 20458118     EISSN: 20458118     Source Type: Journal    
DOI: 10.1186/s12987-015-0015-9     Document Type: Article

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