An improved in vitro blood-brain barrier model: Rat brain endothelial cells co-cultured with astrocytes

Methods in Molecular Biology

Volumn 814, Issue , 2012, Pages 415-430

  Abbott, N Joan ^a   Dolman, Diana E M ^a   Drndarski, Svetlana ^a   Fredriksson, Sarah M ^b  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Astrocyte; Blood brain barrier; Co culture; In vitro model; Permeability; Rat brain endothelial cells; Tight junction

Indexed keywords

ANIMAL; ARTICLE; ASTROCYTE; BLOOD BRAIN BARRIER; BRAIN; CULTURE TECHNIQUE; CYTOLOGY; ENDOTHELIUM CELL; IMPEDANCE; IN VITRO STUDY; METHODOLOGY; PHYSIOLOGY; RAT; TIGHT JUNCTION;

ANIMALS; ASTROCYTES; BLOOD-BRAIN BARRIER; BRAIN; CELL CULTURE TECHNIQUES; ELECTRIC IMPEDANCE; ENDOTHELIAL CELLS; RATS; TIGHT JUNCTIONS;

BOVINAE; RATTUS; RODENTIA;

EID: 84855947240     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-452-0_28     Document Type: Article

References (17)

1. Abbott, N.J., Patabendige, A.A., Dolman, D.E., Yusof, S.R., and Begley, D.J. (2010) Structure and function of the blood-brain barrier. Neurobiol. Dis. 37, 13-25.
2. Abbott, N.J., Rönnbäck, L., and Hansson, E. (2006) Astrocyte-endothelial interactions at the blood-brain barrier. Nat. Rev. Neurosci. 7, 41-53. (Pubitemid 41828930)
3. Deli, M.A., Abrahám, C.S., Kataoka, Y., and Niwa, M. (2005) Permeability studies on in vitro blood-brain barrier models: physiology, pathology, and pharmacology. Cell Mol Neurobiol. 25, 59-127. (Pubitemid 40558585)
4. Nakagawa, S., Deli, M.A., Kawaguchi, H., Shimizudani, T., Shimono, T., Kittel, A. et al. (2009) A new blood-brain barrier model using primary rat brain endothelial cells, pericytes and astrocytes. Neurochem. Int. 54, 253-263.
5. Perrière, N. Demeuse, P., Garcia, E., Regina, A., Debray, M., Andreux, J.P. et al. (2005) Puromycin-based purifi cation of rat brain capillary endothelial cell cultures. Effect on the expression of blood-brain barrier-specifi c properties. J. Neurochem. 93, 279-289. (Pubitemid 40547485)
6. Gaillard, P.J., Voorwinden, L.H., Nielsen, J.L., Ivanov, A., Atsumi, R., Engman, H. et al. (2001) Establishment and functional characterization of an in vitro model of the blood- brain barrier comprising a co-culture of brain capillary endothelial cells and astrocytes. Eur. J. Pharm. Sci. 12, 215-222.
7. Stanness, K.A., Guatteo, E., and Janigro, D. (1996) A dynamic model of the blood-brain barrier " in vitro ". Neurotoxicol. 17, 481-496. (Pubitemid 26321348)
8. Booher, J., and Sensenbrenner, M. (1972) Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in fl ask cultures. Neurobiol. 2, 97-105.
9. Gómez-Lechón, M.J., Iborra, F.J., Azorín, I., Guerri, C., Renau-Piqueras, J. (1992) Cryopreservation of rat astrocytes from primary cultures. J. Tissue Cult. Methods 14, 73-82.
10. Bowman, P.D., Betz, A.L., Ar, D., Wolinsky, J.S., Penney, J.B. et al. (1981) Primary culture of capillary endothelium from rat brain. In Vitro 17, 353-362. (Pubitemid 12251553)
11. Bowman, P.D., Ennis, S.R., Rarey, K.E., Betz, A.L., and Goldstein, G.W. (1983) Brain microvessel endothelial cells in tissue culture: A model for study of blood-brain barrier permeability. Ann. Neurol. 14, 396-402. (Pubitemid 13010039)
12. Hughes, C.C., and Lantos, P.L. (1986) Brain capillary endothelial cells in vitro lack surface IgG Fc receptors. Neurosci. Lett. 68, 100-106. (Pubitemid 16064692)
13. Hughes, C.C., and Lantos, P.L. (1989) Uptake of leucine and alanine by cultured cerebral capillary endothelial cells. Brain Res. 480, 126-132. (Pubitemid 19050205)
14. Abbott, N.J., Hughes, C.C., Revest, P.A., and Greenwood, J. (1992) Development and characterization of a rat brain capillary endothelial culture: towards an in vitro blood-brain barrier. J. Cell Sci. 103, 23-37.
15. Dolman, D., Drndarski S., Abbott, N.J., and Rattray, M. (2005). Induction of aquaporin 1 but not aquaporin 4 messenger RNA in rat primary brain microvessel endothelial cells in culture. J. Neurochem. 93, 825-383.
16. Strom, S.C. and Michalopoulos, G. (1982) Collagen as a substrate for cell growth and differentiation. Methods Enzymol. 82, 544-555.
17. Fredriksson, S.M. (2009) Modulation of the blood-brain barrier by infl ammatory mediators: relevance to multiple sclerosis. PhD Thesis, King's College London, University of London.