Early graft failure of GalTKO pig organs in baboons is reduced by expression of a human complement pathway-regulatory protein

Xenotransplantation

Volumn 22, Issue 4, 2015, Pages 310-316

  Azimzadeh, Agnes M ^a   Kelishadi, Sean S ^a   Ezzelarab, Mohamed B ^b   Singh, Avneesh K ^c   Stoddard, Tiffany ^a   Iwase, Hayato ^b   Zhang, Tianshu ^a   Burdorf, Lars ^a   Sievert, Evelyn ^a   Avon, Chris ^a   Cheng, Xiangfei ^a   Ayares, David ^d   Horvath, Keith A ^c   Corcoran, Philip C ^c   Mohiuddin, Muhammad M ^c   Barth, Rolf N ^a   Cooper, David K C ^b   Pierson, Richard N ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

^c NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^d REVIVICOR INC   (United States)

Author keywords

baboon; complement regulatory protein; pig; xenograft rejection; xenotransplantation; 1 3 galactosyltransferase gene knockout

Indexed keywords

CD20 ANTIBODY; CD40 LIGAND MONOCLONAL ANTIBODY; COBROTOXIN; CORTICOSTEROID; CYTOTOXIC T LYMPHOCYTE ANTIGEN 4 ANTIBODY; DECAY ACCELERATING FACTOR; GALACTOSE; MEMBRANE COFACTOR PROTEIN; MYCOPHENOLATE MOFETIL; REGULATOR PROTEIN; THYMOCYTE ANTIBODY; ALPHA-1,3-GALACTOSYLTRANSFERASE 1, PORCINE; CD46 PROTEIN, HUMAN; DISACCHARIDE; GALACTOSYL-(1-3)GALACTOSE; GALACTOSYLTRANSFERASE; RECOMBINANT PROTEIN;

ADD ON THERAPY; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BABOON; COMPLEMENT SYSTEM; CONTROLLED STUDY; EARLY GRAFT FAILURE; GRAFT FAILURE; GRAFT RECIPIENT; INCIDENCE; NONHUMAN; ORGAN TRANSPLANTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSGENIC PIG; XENOTRANSPLANTATION; ADVERSE EFFECTS; ANIMAL; COMPLEMENT ACTIVATION; DEFICIENCY; GENE INACTIVATION; GENETICS; GRAFT REJECTION; GRAFT SURVIVAL; HEART TRANSPLANTATION; HUMAN; IMMUNOLOGY; KIDNEY TRANSPLANTATION; PIG; PROCEDURES; TRANSGENIC ANIMAL; XENOGRAFT;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; ANTIGENS, CD46; ANTIGENS, CD55; COMPLEMENT ACTIVATION; DISACCHARIDES; GALACTOSYLTRANSFERASES; GENE KNOCKOUT TECHNIQUES; GRAFT REJECTION; GRAFT SURVIVAL; HEART TRANSPLANTATION; HUMANS; KIDNEY TRANSPLANTATION; PAPIO; RECOMBINANT PROTEINS; SWINE; TRANSPLANTATION, HETEROLOGOUS;

EID: 84938081131     PISSN: 0908665X     EISSN: 13993089     Source Type: Journal    
DOI: 10.1111/xen.12176     Document Type: Article

References (34)

1. Ekser B, Ezzelarab M, Hara H, et al.; Clinical xenotransplantation-the next medical revolution? Lancet 2012; 379: 672-683.
2. Good AH, Cooper DK, Malcom AJ, et al.; Identification of carbohydrate structures that bind human antiporcine antibodies: implications for discordant xenografting in man. Transplant Proc 1992; 24: 559-562.
3. Kuwaki K, Tseng YL, Dor FJ, et al.; Heart transplantation in baboons using α1,3-galactosyltransferase gene-knockout pigs as donors: initial experience. Nat Med 2005; 11: 29-31.
4. Tseng YL, Kuwaki K, Dor FJ, et al.; α 1,3-galactosyltransferase gene-knockout pig heart transplantation in baboons with survival approaching six months. Transplantation 2005; 80: 1493-1500.
5. Chen G, Qian H, Starzl T, et al.; Acute rejection is associated with antibodies to non-Galantigens in baboons using Gal-knockout pig kidneys. Nat Med 2005; 11: 1295-1298.
6. Yamada K, Yazawa K, Shimizu A, et al.; Marked prolongation of porcine renal xenograft survival in baboons through the use of α1,3-galactosyltransferase gene-knockout donors and the cotransplantation of vascularized thymic tissue. Nat Med 2005; 11: 32-34.
7. Hara H, Long C, Lin YJ, et al.; In vitro investigation of pig cells for resistance to human antibody-mediated rejection. Transpl Int 2008; 21: 1163-1174.
8. McGregor CG, Ricci D, Miyagi N, et al.; Human CD55 expression blocks hyperacute rejection and restricts complement activation in Gal knockout cardiac xenografts. Transplantation 2012; 93: 686-692.
9. Cooper DK, Hara H, Ezzelarab M, et al.; The potential of genetically-engineered pigs in providing an alternative source of organs and cells for transplantation. J Biomed Res 2013; 27: 249-253.
10. Burdorf L, Stoddard T, Zhang T, et al.; Expression of human CD46 modulates inflammation associated with GalTKO lung xenograft injury. Am J Transplant 2014; 14: 1084-1095.
11. Le Bas-Bernardet S, Tillou X, Branchereau J, et al.; C1-inhibitor and plasma exchange do not prolong the survival of multi-transgenic GalT-KO pig kidney xenografts in baboons. Am J Transplant 2015; 15: 358-370.
12. Ezzelarab M, Garcia B, Azimzadeh A, et al.; The innate immune response and activation of coagulation in α1,3-galactosyltransferase gene-knockout xenograft recipients. Transplantation 2009; 87: 805-812.
13. Azimzadeh AM, Byrne GW, Ezzelarab M, et al.; Development of a consensus protocol to quantify primate anti-non-Gal xenoreactive antibodies using pig aortic endothelial cells. Xenotransplantation 2014; 21: 555-566.
14. Wu G, Pfeiffer S, Schröder C, et al.; Coagulation cascade activation triggers early failure of pig hearts expressing human complement regulatory genes. Xenotransplantation 2007; 14: 34-47.
15. Dalmasso AP, Vercellotti GM, Platt JL, Bach FH,. Inhibition of complement-mediated endothelial cell cytotoxicity by decay-accelerating factor. Potential for prevention of xenograft hyperacute rejection. Transplantation 1991; 52: 530-533.
16. White DJ, Oglesby T, Liszewski MK, et al.; Expression of human decay accelerating factor or membrane cofactor protein genes on mouse cells inhibits lysis by human complement. Transpl Int 1992; 5: 648-650.
17. Cozzi E, White DJG,. The generation of transgenic pigs as potential organ donors for humans. Nat Med 1995; 1: 964-969.
18. Cozzi E, Vial C, Ostlie D, et al.; Maintenance triple immunosuppression with cyclosporin A, mycophenolate sodium and steroids allows prolonged survival of primate recipients of hDAF porcine renal xenografts. Xenotransplantation 2003; 10: 300-310.
19. McGregor CG, Davies WR, Oi K, et al.; Cardiac xenotransplantation: recent preclinical progress with 3-month median survival. J Thorac Cardiovasc Surg 2005; 130: 844-851.
20. Cooper DKC, Koren E, Oriol R,. Genetically engineered pigs. Lancet 1993; 342: 682-683.
21. Phelps CJ, Koike C, Vaught TD, et al.; Production of α1,3-galactosyltransferase-deficient pigs. Science 2003; 299: 411-414.
22. Azimzadeh AM, Kelishadi S, Ezzelarab M, et al.; Early graft failure of GTKO pig organs in baboons is reduced by hCPRP expression. Am J Transplant 2010; 4 (Suppl. 4): 10. (Abstract #499).
23. Gollackner B, Goh SK, Qawi I, et al.; Acute vascular rejection of xenografts: roles of natural and elicited xenoreactive antibodies in activation of vascular endothelial cells and induction of procoagulant activity. Transplantation 2004; 77: 1735-1741.
24. Mohiuddin MM, Corcoran PC, Singh AK, et al.; B-cell depletion extends the survival of GTKO.hCD46Tg pig heart xenografts in baboons for up to 8 months. Am J Transplant 2012; 12: 763-771.
25. Zhou H, Iwase H, Wolf RF, et al.; Are there advantages in the use of specific pathogen-free baboons in pig organ xenotransplantation models? Xenotransplantation 2014; 21: 287-290.
26. Yazaki S, Iwamoto M, Onishi A, et al.; Production of cloned pigs expressing human thrombomodulin in endothelial cells. Xenotransplantation 2012; 19: 82-91.
27. Wuensch A, Baehr A, Bongoni AK, et al.; Regulatory sequences of the porcine THBD gene facilitate endothelial-specific expression of bioactive human thrombomodulin in single- and multitransgenic pigs. Transplantation 2014; 97: 138-147.
28. Wheeler DG, Joseph ME, Mahamud SD, et al.; Transgenic swine: expression of human CD39 protects against myocardial injury. J Mol Cell Cardiol 2012; 52: 958-961.
29. Harris DG, Quinn KJ, French BM, et al.; Meta-analysis of the independent and cumulative effects of multiple genetic modifications on pig lung xenograft performance during ex vivo perfusion with human blood. Xenotransplantation 2015; 22: 102-111; commentary 2015; 22: 112-113.
30. Mohiuddin MM, Singh AK, Corcoran PC, et al.; Genetically engineered pigs and target-specific immunomodulation provide significant graft survival and hope for clinical cardiac xenotransplantation. J Thorac Cardiovasc Surg 2014; 148: 1106-1113; discussion 1113-4.
31. Iwase H, Liu H, Wijkstrom M, et al.; Pig kidney graft survival in a baboon for 136 days: longest life-supporting organ graft survival to date. Xenotransplantation 2015; 22: 302-309.
32. Burdorf L, Zhang T, Stoddard T, et al.; Pilot evaluation of GalTKO.hCD39 lungs in a xenogeneic pig lung perfusion model. International Xenotransplantation Association Meeting (IXA), Venice, Italy, October 2009.
33. Burdorf L, Rybak E, Zhang T, et al.; Human EPCR Expression in GalTKO.hCD46 Lungs Extends Survival Time and Lowers PVR in a Xenogenic Lung Perfusion Model. J Heart Lung Transplant 2013; 32: S137.
34. Harris DG, Gao Z, Sievert EP, et al.; Transgenic Human Thrombomodulin Expression Reduces Xenogeneic Thrombosis: a Promising Means of Reducing Pig Lung Xenograft Thrombotic Injury. J Heart Lung Transplant 2014; 33: S108.