Production of α1,3-galactosyltransferase-deficient pigs

Science

Volumn 299, Issue 5605, 2003, Pages 411-414

  Phelps, Carol J ^a   Koike, Chihiro ^c   Vaught, Todd D ^a   Boone, Jeremy ^a   Wells, Kevin D ^a   Chen, Shu Hung ^a   Ball, Suyapa ^a   Specht, Susan M ^c   Polejaeva, Irina A ^a   Monahan, Jeff A ^a   Jobst, Pete M ^a   Sharma, Sugandha B ^c   Lamborn, Ashley E ^a   Garst, Amy S ^a   Moore, Marilyn ^b   Demetris, Anthony J ^c   Rudert, William A ^c   Bottino, Rita ^c   Bertera, Suzanne ^c   Trucco, Massimo ^c   Starzl, Thomas E ^c   Dai, Yifan ^a   Ayares, David L ^a   more..

^a REVIVICOR INC   (United States)

^b PPL Therapeutics   (United Kingdom)

^c CHILDREN S HOSPITAL OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL ORGANS; CELLS; CLONING; GENES;

XENOANTIGENS;

ENZYMES;

ALPHA 1,3 FUCOSYLTRANSFERASE; ALPHA 1,3 GALACTOSE; ANTIGEN; BACTERIAL TOXIN; EPITOPE; UNCLASSIFIED DRUG;

ENZYME; GENE; PIG; TRANSPLANTATION;

ALLELE; ANIMAL EXPERIMENT; ANTIBIOTIC RESISTANCE; ARTICLE; CELL SELECTION; CONTROLLED STUDY; ENZYME DEFICIENCY; ENZYME SYNTHESIS; EXON; FEMALE; FETUS; GENE DISRUPTION; GENE MUTATION; GRAFT REJECTION; KNOCKOUT GENE; MOLECULAR CLONING; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; SWINE; XENOTRANSPLANTATION;

ALLELES; ANIMALS; BACTERIAL TOXINS; CELL LINE; CLONING, MOLECULAR; CLONING, ORGANISM; DNA, COMPLEMENTARY; EMBRYO TRANSFER; ENTEROTOXINS; FEMALE; FIBROBLASTS; GALACTOSYLTRANSFERASES; GENE TARGETING; GENETIC VECTORS; HELA CELLS; HUMANS; IMMUNOGLOBULIN M; ISLETS OF LANGERHANS TRANSPLANTATION; MICE; MICE, KNOCKOUT; POINT MUTATION; PREGNANCY; SWINE; TRANSFECTION; TRANSPLANTATION, HETEROLOGOUS; TRISACCHARIDES;

ANIMALIA; BACTERIA (MICROORGANISMS); MAMMALIA; SUS SCROFA;

EID: 0347367029     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1078942     Document Type: Article

References (20)

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13. 657A-I11 1-6 cells were electroporated with pPL680 and cultured for 5 days. Cells were exposed to C. difficile toxin A (2 μg/ml) (Techlab, Blacksburg, VA) in growth medium for 2 hours. Toxin A medium was then replaced with fresh growth medium. Medium was changed 7 and 11 days posttransfection to remove the detached cells. One colony (680B1) was harvested 13 days posttransfection for expansion and cryopreservation.
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20. Supported in part by a grant from the National Institute of Standards and Technology Advanced Technology Program to PPL Inc. and by National Institutes of Health grant DK29961 (T.E.S). We thank B. Gragg, W. Lucero, T. Akers, H. Bishop, and J. McPherson for technical contributions to embryo transfer and animal husbandry; J. Cowell-Lucero, J. Hencke, and V. Marshall for help in cell culture and transfection; D. Innes for help in complement lysis assay; J. Profozich and T. Libert at the University of Pittsburgh for technical support; and staff at the Virginia-Maryland Regional College of Veterinary Medicine for performing cesarean delivery and physical examination of piglets. All animal work was done following a protocol approved by the PPL, Inc. (Blacksburg, VA) institutional animal care and use committee.