The role of flagella in Clostridium difficile pathogenicity

Trends in Microbiology

Volumn 23, Issue 5, 2015, Pages 275-282

  Stevenson, Emma ^a   Minton, Nigel P ^a   Kuehne, Sarah A ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Flagellar gene regulation; Toxin expression

Indexed keywords

BACTERIAL TOXIN; BACTERIAL PROTEIN; ENTEROTOXIN; FLAD PROTEIN, BACTERIA; FLGE PROTEIN, BACTERIA; FLIA PROTEIN, BACTERIA; SIGMA FACTOR; TCDA PROTEIN, CLOSTRIDIUM DIFFICILE; TOXB PROTEIN, CLOSTRIDIUM DIFFICILE;

BACTERIAL COLONIZATION; BACTERIAL FLAGELLUM; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL VIRULENCE; FLIA GENE; FLIC GENE; FLID GENE; FLIF GENE; FLIG GENE; FLIM GENE; GENE CONTROL; GENE EXPRESSION; GENETIC VARIABILITY; GLYCOSYLATION; HUMAN; IMMUNE SYSTEM; NONHUMAN; PEPTOCLOSTRIDIUM DIFFICILE; PRIORITY JOURNAL; PROMOTER REGION; REVIEW; ANIMAL; BACTERIUM ADHERENCE; BIOSYNTHESIS; CLOSTRIDIUM INFECTION; FLAGELLUM; GENE EXPRESSION REGULATION; GENETICS; MICROBIOLOGY; MUTATION; PATHOGENICITY; PHYSIOLOGY; ULTRASTRUCTURE; VIRULENCE;

CLOSTRIDIUM DIFFICILE;

ANIMALS; BACTERIAL ADHESION; BACTERIAL PROTEINS; BACTERIAL TOXINS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; ENTEROTOXINS; FLAGELLA; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; MUTATION; SIGMA FACTOR; VIRULENCE;

EID: 84937638188     PISSN: 0966842X     EISSN: 18784380     Source Type: Journal    
DOI: 10.1016/j.tim.2015.01.004     Document Type: Review

References (49)

1. Harshey R.M. Bacterial motility on a surface: many ways to a common goal. Annu. Rev. Microbiol. 2003, 57:249-273.
2. Duan Q., et al. Flagella and bacterial pathogenicity. J. Basic Microbiol. 2013, 53:1-8.
3. Macnab R.M. How bacteria assemble flagella. Annu. Rev. Microbiol. 2003, 57:77-100.
4. Aubry A., et al. Modulation of toxin production by the flagellar regulon in Clostridium difficile. Infect. Immun. 2012, 80:3521-3532.
5. Nachamkin I., et al. Role of Campylobacter jejuni flagella as colonization factors for three-day-old chicks: analysis with flagellar mutants. Appl. Environ. Microbiol. 1993, 59:1269-1273.
6. Richardson K. Roles of motility and flagellar structure in pathogenicity of Vibrio cholerae: analysis of motility mutants in three animal models. Infect. Immun. 1991, 59:2727-2736.
7. Dunne C., et al. Factors that mediate colonization of the human stomach by Helicobacter pylori. World J. Gastroenterol. 2014, 20:5610-5624.
8. Postnova T., et al. Motility mutants of Vibrio cholerae O1 have reduced adherence in vitro to human small intestinal epithelial cells as demonstrated by ELISA. Microbiology 1996, 142:2767-2776.
9. Konkel M.E., et al. Secretion of virulence proteins from Campylobacter jejuni is dependent on a functional flagellar export apparatus. J. Bacteriol. 2004, 186:3296-3303.
10. Jones A.M., et al. Clostridium difficile: a European perspective. J. Infect. 2012, 66:115-128.
11. Tasteyre A., et al. Role of FliC and FliD flagellar proteins of Clostridium difficile in adherence and gut colonization. Infect. Immun. 2001, 69:7937-7940.
12. Baban S.T., et al. The role of flagella in Clostridium difficile pathogenesis: comparison between a non-epidemic and an epidemic strain. PLoS ONE 2013, 8:e73026.
13. Anderson J.K., et al. Sense and sensibility: flagellum-mediated gene regulation. Trends Microbiol. 2010, 18:30-37.
14. Smith T.G., Hoover T.R. Deciphering bacterial flagellar gene regulatory networks in the genomic era. Advances in Applied Microbiology 2009, Vol. 67:257-295. Academic Press.
15. Stabler R., et al. Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium. Genome Biol. 2009, 10:R102.
16. Mirel D.B., et al. Identification of flagellar synthesis regulatory and structural genes in a sigma D-dependent operon of Bacillus subtilis. J. Bacteriol. 1994, 176:4492-4500.
17. El Meouche I., et al. Characterization of the sigD regulon of C. difficile and its positive control of toxin production through the regulation of tcdR. PLoS ONE 2013, 8:e83748.
18. Twine S., et al. Motility and flagellar glycosylation in Clostridium difficile. J. Bacteriol. 2009, 191:7050-7062.
19. Stabler R.A., et al. Comparative phylogenomics of Clostridium difficile reveals clade specificity and microevolution of hypervirulent strains. J. Bacteriol. 2006, 188:7297-7305.
20. Faulds-Pain A., et al. The post-translational modification of the Clostridium difficile flagellin affects motility, cell surface properties and virulence. Mol. Microbiol. 2014, 94:272-289.
21. Aldridge P., Hughes K.T. Regulation of flagellar assembly. Curr. Opin. Microbiol. 2002, 5:160-165.
22. Martin M.J., et al. The agr locus regulates virulence and colonization genes in Clostridium difficile 027. J. Bacteriol. 2013, 195:3672-3681.
23. Valiente E., et al. Emergence of new PCR ribotypes from the hypervirulent Clostridium difficile 027 lineage. J. Med. Microbiol. 2012, 61:49-56.
24. Logan S.M. Flagellar glycosylation - a new component of the motility repertoire?. Microbiology 2006, 152:1249-1262.
25. Guerry P., et al. Changes in flagellin glycosylation affect Campylobacter autoagglutination and virulence. Mol. Microbiol. 2006, 60:299-311.
26. Heap J.T., et al. The ClosTron: a universal gene knock-out system for the genus Clostridium. J. Microbiol. Methods 2007, 70:452-464.
27. Heap J.T., et al. Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker. Nucleic Acids Res. 2012, 40:e59.
28. Ng Y.K., et al. Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles. PLoS ONE 2013, 8:e56051.
29. Dapa T., et al. Multiple factors modulate biofilm formation by the anaerobic pathogen Clostridium difficile. J. Bacteriol. 2013, 195:545-555.
30. Dingle T.C., et al. Mutagenic analysis of the Clostridium difficile flagellar proteins, FliC and FliD, and their contribution to virulence in hamsters. Infect. Immun. 2011, 79:4061-4067.
31. Tasteyre A., et al. Phenotypic and genotypic diversity of the flagellin gene (fliC) among Clostridium difficile isolates from different serogroups. J. Clin. Microbiol. 2000, 38:3179-3186.
32. Tasteyre A., et al. Molecular characterization of fliD gene encoding flagellar cap and its expression among Clostridium difficile isolates from different serogroups. J. Clin. Microbiol. 2001, 39:1178-1183.
33. Wilcox M.H., et al. Changing epidemiology of Clostridium difficile infection following the introduction of a national ribotyping-based surveillance scheme in England. Clin. Infect. Dis. 2012, 55:1056-1063.
34. Goorhuis A., et al. Clostridium difficile PCR ribotype 078: an emerging strain in humans and in pigs?. J. Clin. Microbiol. 2008, 46:1157-1158.
35. Cairns M.D., et al. The continually evolving Clostridium difficile species. Future Microbiol. 2012, 7:945-957.
36. Schneeberg A., et al. Presence of Clostridium difficile PCR ribotype clusters related to 033, 078 and 045 in diarrhoeic calves in Germany. J. Med. Microbiol. 2013, 62:1190-1198.
37. Barketi-Klai A., et al. The flagellin FliC of Clostridium difficile is responsible for pleiotropic gene regulation during in vivo infection. PLoS ONE 2014, 9:e96876.
38. Cartman S.T., et al. Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production. Appl. Environ. Microbiol. 2012, 78:4683-4690.
39. Dupuy B., et al. Clostridium difficile toxin synthesis is negatively regulated by TcdC. J. Med. Microbiol. 2008, 57:685689.
40. Burns D.A., et al. The diverse sporulation characteristics of Clostridium difficile clinical isolates are not associated with type. Anaerobe 2010, 16:618-622.
41. Solomon K. The host immune response to Clostridium difficile infection. Ther. Adv. Infect. Dis. 2013, 1:19-35.
42. Kelly C.P., Kyne L. The host immune response to Clostridium difficile. J. Med. Microbiol. 2011, 60:1070-1079.
43. Ghose C. Clostridium difficile infection in the twenty-first century. Emerg. Microbes Infect. 2013, 2:e62.
44. Hayashi F., et al. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature 2001, 410:1099-1103.
45. Péchiné S., et al. Variability of Clostridium difficile surface proteins and specific serum antibody response in patients with Clostridium difficile-associated disease. J. Clin. Microbiol. 2005, 43:5018-5025.
46. Péchiné S., et al. Diminished intestinal colonization by Clostridium difficile and immune response in mice after mucosal immunization with surface proteins of Clostridium difficile. Vaccine 2007, 25:3946-3954.
47. Jarchum I., et al. Toll-like receptor 5 stimulation protects mice from acute Clostridium difficile colitis. Infect. Immun. 2011, 79:1498-1503.
48. Yoshino Y., et al. Clostridium difficile flagellin stimulates Toll-like receptor 5, and toxin B promotes flagellin-induced chemokine production via TLR5. Life Sci. 2013, 92:211-217.
49. Rutherford K., et al. Artemis: sequence visualization and annotation. Bioinformatics 2000, 16:944-945.