Clostridium difficile flagellin stimulates toll-like receptor 5, and toxin B promotes flagellin-induced chemokine production via TLR5

Life Sciences

Volumn 92, Issue 3, 2013, Pages 211-217

  Yoshino, Yusuke ^a,b   Kitazawa, Takatoshi ^b   Ikeda, Mahoko ^a   Tatsuno, Keita ^a   Yanagimoto, Shintaro ^a   Okugawa, Shu ^a   Yotsuyanagi, Hiroshi ^a   Ota, Yasuo ^b  

^a UNIVERSITY OF TOKYO   (Japan)

^b TEIKYO UNIVERSITY   (Japan)

Author keywords

Clostridium difficile; Flagellin; Toll like receptor 5; Toxin B

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN B; FLAGELLIN; INTERLEUKIN 8; MACROPHAGE INFLAMMATORY PROTEIN 3ALPHA; MITOGEN ACTIVATED PROTEIN KINASE P38; TOLL LIKE RECEPTOR 5;

ARTICLE; CELL STIMULATION; CELL STRAIN CACO 2; CELL STRAIN HEK293; CELL STRAIN HT29; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; CYTOKINE PRODUCTION; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM CELL; NONHUMAN; PROTEIN PHOSPHORYLATION; SALMONELLA TYPHIMURIUM; TRANSIENT TRANSFECTION;

BACTERIAL PROTEINS; BACTERIAL TOXINS; CACO-2 CELLS; CHEMOKINE CCL20; CLOSTRIDIUM DIFFICILE; EPITHELIAL CELLS; FLAGELLIN; HEK293 CELLS; HUMANS; INTERLEUKIN-8; INTESTINAL MUCOSA; NF-KAPPA B; P38 MITOGEN-ACTIVATED PROTEIN KINASES; TOLL-LIKE RECEPTOR 5; TRANSFECTION;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM DIFFICILE;

EID: 84873413568     PISSN: 00243205     EISSN: 18790631     Source Type: Journal    
DOI: 10.1016/j.lfs.2012.11.017     Document Type: Article

References (37)

1. M.T. Abreu, M. Fukata, and M. Arditi TLR signaling in the gut in health and disease J Immunol 174 2005 4453 4460
2. A. Bandyopadhaya, M. Sarkar, and K. Chaudhuri IL-1beta expression in Int407 is induced by flagellin of Vibrio cholerae through TLR5 mediated pathway Microb Pathog 44 2008 524 536
3. J.G. Bartlett, and D.N. Gerding Clinical recognition and diagnosis of Clostridium difficile infection Clin Infect Dis 46 Suppl. 1 2008 S12 S18
4. M. Delmee, V. Avesani, N. Delferriere, and G. Burtonboy Characterization of flagella of Clostridium difficile and their role in serogrouping reactions J Clin Microbiol 28 1990 2210 2214
5. M.A. Donnelly, and T.S. Steiner Two nonadjacent regions in enteroaggregative Escherichia coli flagellin are required for activation of toll-like receptor 5 J Biol Chem 277 2002 40456 40461
6. D. Drudy, S. Fanning, and L. Kyne Toxin A-negative, toxin B-positive Clostridium difficile Int J Infect Dis 11 2007 5 10
7. T. Du, and M.J. Alfa Translocation of Clostridium difficile toxin B across polarized Caco-2 cell monolayers is enhanced by toxin A Can J Infect Dis 15 2004 83 88
8. B. Fournier, I.R. Williams, A.T. Gewirtz, and A.S. Neish Toll-like receptor 5-dependent regulation of inflammation in systemic Salmonella enterica serovar Typhimurium infection Infect Immun 77 2009 4121 4129
9. A.T. Gewirtz, T.A. Navas, S. Lyons, P.J. Godowski, and J.L. Madara Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression J Immunol 167 2001 1882 1885
10. L.M. Harrison, P. Rallabhandi, J. Michalski, X. Zhou, S.R. Steyert, and S.N. Vogel Vibrio cholerae flagellins induce Toll-like receptor 5-mediated interleukin-8 production through mitogen-activated protein kinase and NF-kappaB activation Infect Immun 76 2008 5524 5534
11. F. Hayashi, K.D. Smith, A. Ozinsky, T.R. Hawn, E.C. Yi, and D.R. Goodlett The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5 Nature 410 2001 1099 1103
12. I. Jarchum, M. Liu, L. Lipuma, and E.G. Pamer Toll-like receptor 5 stimulation protects mice from acute Clostridium difficile colitis Infect Immun 79 2011 1498 1503
13. C.P. Kelly, and J.T. LaMont Clostridium difficile - more difficult than ever N Engl J Med 359 2008 1932 1940
14. M.A. Khan, J. Kang, and T.S. Steiner Enteroaggregative Escherichia coli flagellin-induced interleukin-8 secretion requires Toll-like receptor 5-dependent p38 MAP kinase activation Immunology 112 2004 651 660
15. S.A. Kuehne, S.T. Cartman, and N.P. Minton Both, toxin A and toxin B, are important in Clostridium difficile infection Gut Microbes 2 2011 252 255
16. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
17. S.E. Lee, S.Y. Kim, B.C. Jeong, Y.R. Kim, S.J. Bae, and O.S. Ahn A bacterial flagellin, Vibrio vulnificus FlaB, has a strong mucosal adjuvant activity to induce protective immunity Infect Immun 74 2006 694 702
18. D.M. Lyerly, H.C. Krivan, and T.D. Wilkins Clostridium difficile: its disease and toxins Clin Microbiol Rev 1 1988 1 18
19. S. Lyons, L. Wang, J.E. Casanova, S.V. Sitaraman, D. Merlin, and A.T. Gewirtz Salmonella Typhimurium transcytoses flagellin via an SPI2-mediated vesicular transport pathway J Cell Sci 117 2004 5771 5780
20. D. Lyras, J.R. O'Connor, P.M. Howarth, S.P. Sambol, G.P. Carter, and T. Phumoonna Toxin B is essential for virulence of Clostridium difficile Nature 458 2009 1176 1179
21. J.L. Madara, J. Stafford, D. Barenberg, and S. Carlson Functional coupling of tight junctions and microfilaments in T84 monolayers Am J Physiol 254 1988 G416 G423
22. K.G. Murthy, A. Deb, S. Goonesekera, C. Szabo, and A.L. Salzman Identification of conserved domains in Salmonella Muenchen flagellin that are essential for its ability to activate TLR5 and to induce an inflammatory response in vitro J Biol Chem 279 2004 5667 5675
23. C. Pontier, J. Pachot, R. Botham, B. Lenfant, and P. Arnaud HT29-MTX and Caco-2/TC7 monolayers as predictive models for human intestinal absorption: role of the mucus layer J Pharm Sci 90 2001 1608 1619
24. M.D. Redelings, F. Sorvillo, and L. Mascola Increase in Clostridium difficile-related mortality rates, United States, 1999-2004 Emerg Infect Dis 13 2007 1417 1419
25. J.M. Reichhart TLR5 takes aim at bacterial propeller Nat Immunol 4 2003 1159 1160
26. S.H. Rhee, E. Im, M. Riegler, E. Kokkotou, M. O'Brien, and C. Pothoulakis Pathophysiological role of Toll-like receptor 5 engagement by bacterial flagellin in colonic inflammation Proc Natl Acad Sci U S A 102 2005 13610 13615
27. S.P. Sambol, J.K. Tang, M.M. Merrigan, S. Johnson, and D.N. Gerding Infection of hamsters with epidemiologically important strains of Clostridium difficile J Infect Dis 183 2001 1760 1766
28. B. Schmeck, P.D. N'Guessan, M. Ollomang, J. Lorenz, J. Zahlten, and B. Opitz Legionella pneumophila-induced NF-kappaB- and MAPK-dependent cytokine release by lung epithelial cells Eur Respir J 29 2007 25 33
29. K.D. Smith, E. Andersen-Nissen, F. Hayashi, K. Strobe, M.A. Bergman, and S.L. Barrett Toll-like receptor 5 recognizes a conserved site on flagellin required for protofilament formation and bacterial motility Nat Immunol 4 2003 1247 1253
30. T.S. Steiner How flagellin and toll-like receptor 5 contribute to enteric infection Infect Immun 75 2007 545 552
31. K. Takeda, and S. Akira Toll-like receptors Curr Protoc Immunol 77 2007 1 14 [Chapter 14:Unit 14 2]
32. T. Tallant, A. Deb, N. Kar, J. Lupica, M.J. de Veer, and J.A. DiDonato Flagellin acting via TLR5 is the major activator of key signaling pathways leading to NF-kappa B and proinflammatory gene program activation in intestinal epithelial cells BMC Microbiol 4 2004 33
33. A. Tasteyre, M.C. Barc, T. Karjalainen, P. Dodson, S. Hyde, and P. Bourlioux A Clostridium difficile gene encoding flagellin Microbiology 146 Pt 4 2000 957 966
34. D. van der Waaij The ecology of the human intestine and its consequences for overgrowth by pathogens such as Clostridium difficile Annu Rev Microbiol 43 1989 69 87
35. D.E. Voth, and J.D. Ballard Clostridium difficile toxins: mechanism of action and role in disease Clin Microbiol Rev 18 2005 247 263
36. Y. Yu, H. Zeng, S. Lyons, A. Carlson, D. Merlin, and A.S. Neish TLR5-mediated activation of p38 MAPK regulates epithelial IL-8 expression via posttranscriptional mechanism Am J Physiol Gastrointest Liver Physiol 285 2003 G282 G290
37. Z. Zhang, W. Reenstra, D.J. Weiner, J.P. Louboutin, and J.M. Wilson The p38 mitogen-activated protein kinase signaling pathway is coupled to Toll-like receptor 5 to mediate gene regulation in response to Pseudomonas aeruginosa infection in human airway epithelial cells Infect Immun 75 2007 5985 5992