Single active-site mutants are sufficient to enhance serine:pyruvate α-transaminase activity in an ω-transaminase

FEBS Journal

Volumn 282, Issue 13, 2015, Pages 2512-2526

  Deszcz, Dawid ^a   Affaticati, Pierre ^a   Ladkau, Nadine ^a   Gegel, Alex ^a   Ward, John M ^a   Hailes, Helen C ^a   Dalby, Paul A ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

aminotransferase; biocatalysis; directed evolution; substrate specificity; transaminase

Indexed keywords

ALPHA TRANSAMINASE TYPE SERINE PYRUVATE AMINOTRANSFERASE; AMINOTRANSFERASE; OMEGA TRANSAMINASE; SERINE; UNCLASSIFIED DRUG; SERINE-PYRUVATE AMINOTRANSFERASE;

ARTICLE; BINDING SITE; CHROMOBACTERIUM VIOLACEUM; CONTROLLED STUDY; CRYSTALLOGRAPHY; ENTROPY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; HIGH THROUGHPUT SCREENING; MOLECULAR DOCKING; MOLECULAR EVOLUTION; NONHUMAN; PHYLOGENY; POINT MUTATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN STRUCTURE; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; CHEMICAL STRUCTURE; CHEMISTRY; HYDROGEN BOND; METABOLISM; MUTATION;

CHROMOBACTERIUM VIOLACEUM;

CATALYTIC DOMAIN; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROGEN BONDING; MODELS, MOLECULAR; MUTATION; PHYLOGENY; SUBSTRATE SPECIFICITY; TRANSAMINASES;

EID: 84934286966     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.13293     Document Type: Article

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