Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde

Journal of Biotechnology

Volumn 134, Issue 3-4, 2008, Pages 240-245

  Hibbert, Edward G ^a   Senussi, Tarik ^a   Smith, Mark E B ^a   Costelloe, Sean J ^a   Ward, John M ^a   Hailes, Helen C ^a   Dalby, Paul A ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Biocatalysis; Directed evolution; Phylogenetics; Protein engineering; Saturation mutagenesis; Transketolase

Indexed keywords

BIOCATALYSIS; PHYLOGENETICS; PROTEIN ENGINEERING; SATURATION MUTAGENESIS; TRANSKETOLASE; TRANSKETOLASE SUBSTRATES;

ALDEHYDES; BIOCATALYSTS; CATALYSIS; ENZYME ACTIVITY; HYDROXYLATION; MUTAGENESIS;

SUBSTRATES;

ALDEHYDE; GLYCOALDEHYDE; MUTANT PROTEIN; PROPIONALDEHYDE; TRANSKETOLASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING;

ACETALDEHYDE; ALDEHYDES; AMINO ACID SEQUENCE; BINDING SITES; CATALYSIS; CONSERVED SEQUENCE; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENE LIBRARY; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY; TRANSKETOLASE;

EID: 41549158567     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.01.018     Document Type: Article

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