Redesigning and characterizing the substrate specificity and activity of Vibrio fluvialis aminotransferase for the synthesis of imagabalin

Protein Engineering, Design and Selection

Volumn 26, Issue 1, 2013, Pages 25-33

  Midelfort, Katarina S ^a   Kumar, Rajesh ^a   Han, Seungil ^a   Karmilowicz, Michael J ^a   McConnell, Kevin ^a   Gehlhaar, Daniel K ^a   Mistry, Anil ^a   Chang, Jeanne S ^a   Anderson, Marie ^a   Villalobos, Alan ^b   Minshull, Jeremy ^b   Govindarajan, Sridhar ^b   Wong, John W ^a  

^a PFIZER INC   (United States)

^b DNA20 Inc   (United States)

Author keywords

ketoesters; crystal structure; enzyme engineering; protein GPS; Vibrio fluvialis aminotransferase

Indexed keywords

AMINOTRANSFERASE; ENZYME ENGINEERING; ENZYME PERFORMANCE; KETO ESTER; PROTEIN ENGINEERING; REACTION VELOCITIES; SUBSTRATE SPECIFICITY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; VIBRIO FLUVIALIS; WILD TYPES;

CRYSTAL STRUCTURE; GENETIC ENGINEERING; LIQUID CHROMATOGRAPHY;

ENZYMES;

(3,5)ETHYL 3 AMINO 5 METHYLOCTANOATE; ACETOPHENONE; AMINOTRANSFERASE; ETHYL 5 METHYL 3 OXOOCTANOATE; IMAGABALIN; OCTANOIC ACID; UNCLASSIFIED DRUG;

ARTICLE; BIOINFORMATICS; CATALYSIS; CRYSTAL STRUCTURE; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME SPECIFICITY; GENETIC VARIABILITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; TRANSAMINATION; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; VELOCITY; VIBRIO FLUVIALIS; WILD TYPE;

AMINO ACIDS; CAPRYLATES; KINETICS; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TRANSAMINASES; VIBRIO;

VIBRIO FLUVIALIS;

EID: 84871233538     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs065     Document Type: Article

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