A toolbox approach for the rapid evaluation of multi-step enzymatic syntheses comprising a 'mix and match' E. coli expression system with microscale experimentation

Biocatalysis and Biotransformation

Volumn 29, Issue 5, 2011, Pages 192-203

  Rios Solis, L ^a   Halim, M ^a   Cazares, A ^a   Morris, P ^a   Ward, J M ^a   Hailes, H C ^a   Dalby, P A ^a   Baganz, F ^a   Lye, G J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Biocatalyst; Chiral amino alcohols; De novo pathway; Multi step enzymatic synthesis; Transaminase; Transketolase

Indexed keywords

CHIRAL AMINO ALCOHOLS; DE NOVO PATHWAY; MULTI-STEP; TRANSAMINASE; TRANSKETOLASE;

ALCOHOLS; AMINES; BIOCATALYSTS; CHEMICAL BONDS; ENZYMES; ESCHERICHIA COLI; EXPERIMENTS; LIBRARIES; MICROCHANNELS;

SUBSTRATES;

2 AMINO 1,3,4 BUTANETRIOL; 2 AMINOPENTANE 1,3 DIOL; ALCOHOL; ALIPHATIC AMINE; AMINE; AMINOTRANSFERASE; ISOPROPYLAMINE; N METHYLBENZYLAMINE; TRANSKETOLASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; AUTOMATION; BIOCATALYST; BIOTRANSFORMATION; CATALYSIS; CELL LYSATE; CHROMOBACTERIUM VIOLACEUM; DIASTEREOISOMER; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXPERIMENTAL STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PLASMID; STEREOISOMERISM;

CHROMOBACTERIUM VIOLACEUM;

EID: 80053286493     PISSN: 10242422     EISSN: 10292446     Source Type: Journal    
DOI: 10.3109/10242422.2011.609589     Document Type: Article

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