Physical supports from liver cancer cells are essential for differentiation and remodeling of endothelial cells in a HepG2-HUVEC co-culture model

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Chiew, Geraldine Giap Ying ^a   Fu, Afu ^a   Perng Low, Kar ^a   Luo, Kathy Qian ^a  

^a Nanyang Technological University   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ANGIOGENESIS INHIBITOR; COLLAGEN; CONDITIONED MEDIUM; DRUG COMBINATION; ENDOTHELIAL CELL GROWTH FACTOR; LAMININ; MATRIGEL; PROTEOGLYCAN; VASCULOTROPIN A;

ANGIOGENESIS; CELL COMMUNICATION; CELL DIFFERENTIATION; CELL LINE; COCULTURE; CONDITIONED MEDIUM; DRUG COMBINATION; DRUG EFFECTS; ENDOTHELIUM CELL; EXTRACELLULAR MATRIX; HEP-G2 CELL LINE; HUMAN; LIVER CELL CARCINOMA; LIVER TUMOR; METABOLISM; NEOVASCULARIZATION, PATHOLOGIC; PATHOLOGY; PHYSIOLOGY; PROCEDURES; TUMOR CELL LINE; UMBILICAL VEIN ENDOTHELIAL CELL; VASCULAR ENDOTHELIUM;

ANGIOGENESIS INHIBITORS; CARCINOMA, HEPATOCELLULAR; CELL COMMUNICATION; CELL DIFFERENTIATION; CELL LINE; CELL LINE, TUMOR; COCULTURE TECHNIQUES; COLLAGEN; CULTURE MEDIA, CONDITIONED; DRUG COMBINATIONS; ENDOTHELIAL CELLS; ENDOTHELIAL GROWTH FACTORS; ENDOTHELIUM, VASCULAR; EXTRACELLULAR MATRIX; HEP G2 CELLS; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; LAMININ; LIVER NEOPLASMS; NEOVASCULARIZATION, PATHOLOGIC; NEOVASCULARIZATION, PHYSIOLOGIC; PROTEOGLYCANS; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 84930965735     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep10801     Document Type: Article

References (45)

1. Folkman, J. Role of angiogenesis in tumor growth and metastasis. Seminars in oncology 29, 15-18, doi:10.1053/sonc.2002.37263 (2002).
2. Khodarev, N. N. et al. Tumour-endothelium interactions in co-culture: coordinated changes of gene expression profles and phenotypic properties of endothelial cells. Journal of cell science 116, 1013-1022 (2003).
3. Szot, C. S., Buchanan, C. F., Freeman, J. W. & Rylander, M. N. In vitro angiogenesis induced by tumor-endothelial cell co-culture in bilayered, collagen I hydrogel bioengineered tumors. Tissue engineering. Part C, Methods 19, 864-874 doi:10.1089/ten. TEC.2012.0684 (2013).
4. Carmeliet, P. VEGF as a key mediator of angiogenesis in cancer. Oncology 69 Suppl 3, 4-10, doi:10.1159/000088478 (2005).
5. Garber, K. Angiogenesis inhibitors sufer new setback. Nat Biotech 20, 1067-1068 (2002).
6. Bergers, G. & Hanahan, D. Modes of resistance to anti-angiogenic therapy. Nat Rev Cancer 8, 592-603 (2008).
7. Llovet, J. M. & Bruix, J. Molecular targeted therapies in hepatocellular carcinoma. Hepatology (Baltimore, Md.) 48, 1312-1327, doi:10.1002/hep.22506 (2008).
8. Liu, L. et al. Sorafenib Blocks the RAF/MEK/ERK Pathway, Inhibits Tumor Angiogenesis, and Induces Tumor Cell Apoptosis in Hepatocellular Carcinoma Model PLC/PRF/5. Cancer Research 66, 11851-11858, doi:10.1158/0008-5472.can-06-1377 (2006).
9. Yang, X.-M. et al. Role of PI3K/Akt and MEK/ERK in Mediating Hypoxia-Induced Expression of HIF-1α and VEGF in Laser-Induced Rat Choroidal Neovascularization. Investigative Ophthalmology & Visual Science 50, 1873-1879, doi:10.1167/iovs.08-2591 (2009).
10. Kleinman, H. K. & Martin, G. R. Matrigel: Basement membrane matrix with biological activity. Seminars in Cancer Biology 15, 378-386 (2005).
11. Stupack, D. G. & Cheresh, D. A. ECM remodeling regulates angiogenesis: endothelial integrins look for new ligands. Science's STKE: signal transduction knowledge environment 2002, pe7, doi:10.1126/stke.2002.119.pe7 (2002).
12. Weis, S. M. & Cheresh, D. A. Tumor angiogenesis: molecular pathways and therapeutic targets. Nat Med 17, 1359-1370 (2011).
13. Chwalek, K., Tsurkan, M. V., Freudenberg, U. & Werner, C. Glycosaminoglycan-based hydrogels to modulate heterocellular communication in in vitro angiogenesis models. Sci. Rep. 4, doi:10.1038/srep04414 (2014).
14. Lupo, G. et al. An in v i tro retinoblastoma human triple culture model of angiogenesis: A modulatory efect of TGF-β. Cance r Letters 354, 181-188, doi:10.1016/j.canlet.2014.08.004 (2014).
15. Zervantonakis, I. K. et al. Tree-dimensional microfuidic model for tumor cell intravasation and endothelial barrier function. Proceedings of the National Academy of Sciences 109, 13515-13520 (2012).
16. Zheng, C. et al. Quantitative Study of the Dynamic Tumor-Endothelial Cell Interactions through an Integrated Microfuidic Coculture System. Analytical Chemistry 84, 2088-2093, doi:10.1021/ac2032029 (2012).
17. Chen, Z. et al. In vitro angiogenesis by human umbilical vein endothelial cells (HUVEC) induced by three-dimensional co-culture with glioblastoma cells. J Neurooncol 92, 121-128, doi:10.1007/s11060-008-9742-y (2009).
18. Luo, K. Q., Yu, V. C., Pu, Y. & Chang, D. C. Application of the fuorescence resonance energy transfer method for studying the dynamics of caspase-3 activation during UV-induced apoptosis in living HeLa cells. Biochemical and biophysical research communications 283, 1054-1060, doi:10.1006/bbrc.2001.4896 (2001).
19. Zhu, X., Fu, A. & Luo, K. Q. A high-throughput fuorescence resonance energy transfer (FRET)-based endothelial cell apoptosis assay and its application for screening vascular disrupting agents. Biochemical and biophysical research communications 418, 641-646, doi:10.1016/j.bbrc.2012.01.066 (2012).
20. Anand, P., Fu, A., Teoh, S. H. & Luo, K. Q. Application of a Fluorescence Resonance Energy Transfer (FRET)-based Biosensor for Detection of Drug-induced Apoptosis in a 3D Breast Tumor Model. Biotechnology and Bioengineering, n/a-n/a, doi:10.1002/bit.25572 (2015).
21. Yu, J. Q., Liu, X. F., Chin, L. K., Liu, A. Q. & Luo, K. Q. Study of endothelial cell apoptosis using fuorescence resonance energy transfer (FRET) biosensor cell line with hemodynamic microfuidic chip system. Lab on a chip 13, 2693-2700, doi:10.1039/c3lc50105a (2013).
22. Liu, X. F., Yu, J. Q., Dalan, R., Liu, A. Q. & Luo, K. Q. Biological factors in plasma from diabetes mellitus patients enhance hyperglycaemia and pulsatile shear stress-induced endothelial cell apoptosis. Integrative biology: quantitative biosciences from nano to macro 6, 511-522, doi:10.1039/c3ib40265g (2014).
23. Trevor, K. T., McGuire, J. G. & Leonova, E. V. Association of vimentin intermediate flaments with the centrosome. Journal of cell science 108, 343-356 (1995).
24. Mendez, M. G., Kojima, S.-I. & Goldman, R. D. Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. The FASEB Journal 24, 1838-1851, doi:10.1096/f.09-151639 (2010).
25. Jochems, C. E., van der Valk, J. B., Stafeu, F. R. & Baumans, V. The use of fetal bovine serum: ethical or scientifc problem? Alternatives to laboratory animals: ATLA 30, 219-227 (2002).
26. Graupera, M. et al. Angiogenesis selectively requires the p110[agr] isoform of PI3K to control endothelial cell migration. Nature 453, 662-666, doi:10.1038/nature06892 (2008).
27. Uchida, C., Gee, E., Ispanovic, E. & Haas, T. L. JNK as a positive regulator of angiogenic potential in endothelial cells. Cell biology international 32, 769-776, doi:10.1016/j.cellbi.2008.03.005 (2008).
28. Matsumoto, T., Turesson, I., Book, M., Gerwins, P. & Claesson-Welsh, L. p38 MAP kinase negatively regulates endothelial cell survival, proliferation, and diferentiation in FGF-2-stimulated angiogenesis. The Journal of cell biology 156, 149-160, doi:10.1083/jcb.200103096 (2002).
29. Issbrücker, K. et al. p38 MAP kinase-a molecular switch between VEGF-induced angiogenesis and vascular hyperpermeability. The FASEB Journal 17, 262-264, doi:10.1096/f.02-0329fe (2003).
30. Bryan, B. A. et al. RhoA/ROCK signaling is essential for multiple aspects of VEGF-mediated angiogenesis. The FASEB Journal 24, 3186-3195, doi:10.1096/f.09-145102 (2010).
31. Kroll, J. et al. Inhibition of Rho-dependent kinases ROCK I/II activates VEGF-driven retinal neovascularization and sprouting angiogenesis. American journal of physiology. Heart and circulatory physiology 296, H893-899, doi:10.1152/ajpheart.01038.2008 (2009).
32. Kaur, S. et al. RhoJ/TCL Regulates Endothelial Motility and Tube Formation and Modulates Actomyosin Contractility and Focal Adhesion Numbers. Arteriosclerosis, Trombosis, and Vascular Biology 31, 657-664, doi:10.1161/atvbaha.110.216341 (2011).
33. Xu, H. et al. Protein kinase C alpha promotes angiogenic activity of human endothelial cells via induction of vascular endothelial growth factor. Cardiovascular research 78, 349-355, doi:10.1093/cvr/cvm085 (2008).
34. Frisch, S. M. & Ruoslahti, E. Integrins and anoikis. Current opinion in cell biology 9, 701-706 (1997).
35. Abdollahi, A. et al. SU5416 and SU6668 Attenuate the Angiogenic Efects of Radiation-induced Tumor Cell Growth Factor Production and Amplify the Direct Anti-endothelial Action of Radiation in Vi tro. Cancer Research 63, 3755-3763 (2003).
36. Erber, R. et al. Combined inhibition of VEGF and PDGF signaling enforces tumor vessel regression by interfering with pericyte-mediated endothelial cell survival mechanisms. FASEB journal: ofcial publication of the Federation of American Societies for Experimental Biology 18, 338-340, doi:10.1096/f.03-0271fe (2004).
37. Kasuya, J., Sudo, R., Mitaka, T., Ikeda, M. & Tanishita, K. Hepatic stellate cell-mediated three-dimensional hepatocyte and endothelial cell triculture model. Tissue engineering. Part A 17, 361-370, doi:10.1089/ten.TEA.2010.0033 (2011).
38. Inamori, M., Mizumoto, H. & Kajiwara, T. An approach for formation of vascularized liver tissue by endothelial cell-covered hepatocyte spheroid integration. Tissue engineering. Part A 15, 2029-2037, doi:10.1089/ten.tea.2008.0403 (2009).
39. Harimoto, M. et al. Novel approach for achieving double-layered cell sheets co-culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature-responsive culture dishes. J Biomed Mater Res 62, 464-470, doi:10.1002/jbm.10228 (2002).
40. Takayama, G., Taniguchi, A. & Okano, T. Identifcation of diferentially expressed genes in hepatocyte/endothelial cell co-culture system. Tissue engineering 13, 159-166, doi:10.1089/ten.2006.0143 (2007).
41. Amoh, Y. et al. Visualization of nascent tumor angiogenesis in lung and liver metastasis by diferential dual-color fuorescence imaging in nestin-linked-GFP mice. Clinical & experimental metastasis 23, 315-322, doi:10.1007/s10585-006-9018-x (2006).
42. Jain, R. K., Munn, L. L. & Fukumura, D. Dissecting tumour pathophysiology using intravital microscopy. Nat Rev Cancer 2, 266-276 (2002).
43. Ma, J. & Waxman, D. J. Combination of antiangiogenesis with chemotherapy for more efective cancer treatment. Mol Cancer Ter 7, 3670-3684, doi:10.1158/1535-7163.mct-08-0715 (2008).
44. Donovan, D., Brown, N. J., Bishop, E. T. & Lewis, C. E. Comparison of three in vitro human 'angiogenesis' assays with capillaries formed in vivo. Angiogenesis 4, 113-121, doi:10.1023/A:1012218401036 (2001).
45. Carpentier G, M. M., Courty, J. & Cascone I. Angiogenesis Analyzer for ImageJ. 4th ImageJ User and Developer Conference Proceedings, Mondorf-les-Bains, Luxembourg. Luxembourg Institute of Science and Technology (2012, October 26-28).