Novel approach for achieving double-layered cell sheets co-culture: Overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature-responsive culture dishes

Journal of Biomedical Materials Research

Volumn 62, Issue 3, 2002, Pages 464-470

  Harimoto, Masami ^a   Yamato, Masayuki ^a   Hirose, Motohiro ^a   Takahashi, Chie ^a   Isoi, Yuki ^a   Kikuchi, Akihiko ^a   Okano, Teruo ^a  

^a TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

Author keywords

Double layered co culture; Endothelial cell sheet; Hepatocyte function; Thermo responsive culture surface

Indexed keywords

BIOLOGICAL ORGANS; CELL CULTURE; CELLS; GRAFTS; MONOLAYERS;

TISSUE ENGINEERING;

BIOMEDICAL ENGINEERING;

ARTICLE; CELL DIFFERENTIATION; CELL SHAPE; CELL TYPE; CYTOARCHITECTURE; ENDOTHELIUM CELL; KIDNEY EPITHELIUM; LIVER FUNCTION; LIVER PARENCHYMA; MONOLAYER CULTURE; THERMOREGULATION; TISSUE CULTURE CELL; TISSUE ENGINEERING;

COCULTURE TECHNIQUES; ENDOTHELIUM, VASCULAR; EQUIPMENT AND SUPPLIES; HEPATOCYTES; HUMANS; MICROSCOPY, FLUORESCENCE; TEMPERATURE;

EID: 0037114401     PISSN: 00219304     EISSN: None     Source Type: Journal    
DOI: 10.1002/jbm.10228     Document Type: Article

References (22)

1. Fawcett DW. A Textbook of Histology, 11 Ed. Philadelphia, PA: W. B. Sanders; 1986.
2. Bhatia SN, Yarmush ML, Toner M. Controlling cell interactions by micropatterning in co-cultures: Hepatocytes and 3T3 fibroblasts. J Biomed Mater Res 1997;34:189-199.
3. Yamada N, Okano T, Sakai H, Karikusa F, Sawasaki Y, Sakurai Y. Thermo-responsive polymeric surfaces; Control of attachment and detachment of cultured cells. Makromol Chem Rapid Commun 1990;11:571-576.
4. Yamato M, Kwon OH, Hirose M, Kikuchi A, Okano T. Novel patterned cell co-culture utilizing thermally responsive grafted polymer surfaces. J Biomed Mater Res 2001;55:137-140.
5. Yamato M, Konno C, Utsumi M, Kushida A, Hirose M, Kikuchi A, Okano T. Thermally responsive polymer-grafted surfaces allows patterned cell seeding and co-culture. Biomaterials, in press.
6. Okano T, Yamada N, Sakai H, Sakurai Y. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly (N-isopropylacrylamide). J Biomed Mater Res 1993;27:1243-1251.
7. Yamato M, Utsumi M, Kushida A, Konno C, Kikuchi A, Okano T. Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature. Tissue Eng. In press.
8. Shimizu T, Yamato M, Kikuchi A, Okano T. Two-dimensional manipulation of cardiac myocyte sheets utilizing temperature-responsive culture dishes augments the pulsatile activity. Tissue Eng. In press.
9. Kushida A, Yamato M, Konno C, Kikuchi A, Sakurai Y, Okano T. Temperature-responsive culture dishes allow non-enzymatic harvest of differentiated Madin-Darby canine kidey (MDCK) cell sheets. J Biomed Mater Res 2000;51:216-223.
10. Kushida A, Yamato M, Konno C, Kikuchi A, Sakurai Y, Okano T. Decrease in culture temperature releases monolayer endothelial cell sheets together with deposited fibronectin matrix from temperature-responsive culture surfaces. J Biomed Mater Res 1999;45:355-362.
11. Kushida A, Yamato M, Kikuchi A, Okano T. Two-dimensional manipulation of differentiated Madin-Darby canine kidney (MDCK) cell sheets: The noninvasive harvest from temperature-responsive culture dishes and transfer to other surfaces. J Biomed Mater Res 2001;54:37-46.
12. Hirose M, Kwon OH, Yamato M, Kikuchi A, Okano T. Creation of designed cell sheets that are noninvasively harvested and moved to another surface. Biomacromolecules 2000;1:377-381.
13. Tateno C, Yoshizato K. Long-term cultivation of adult rat hepatocytes that undergo multiple cell divisions and express normal parenchymal phenotypes. Am J Pathol 1996;148:383-392.
14. Bhatia SN, Balis UJ, Yarmush ML, Toner M. Effect of cell-cell interactions in preservation of cellular phenotype: Cocultivation of hepatocytes and nonparenchymal cells. FASEB J 1999;13:1883-1900.
15. Mesnil M, Fraslin JM, Piccoli C, Yamasaki H, Guguen- Guillouzo C. Cell contact but not junctional communication (dye coupling) with biliary epithelial cells is required for hepatocytes to maintain differentiated functions. Exp Cell Res 1987;173:524-533.
16. Bhatia SN, Toner M, Tompkins RG, Yarmush ML. Selective adhesion of hepatocytes on patterned surfaces. Ann N Y Acad Sci 1994;745:187-209.
17. Dunn JCY, Tompkins RG, Yarmush ML. Hepatocytes in collagen sandwich: Evidence for transcriptional and translational regulation. J Cell Biol 1992;116:1043-1053.
18. Ezzell RM, Toner M, Hendricks K, Dunn JCY, Tompkins RG, Yarmush ML. Effect of collagen gel configuration on the cytoskeleton in cultured rat hepatocytes. Exp Cell Res 1993;208:442-452.
19. Lee J, Morgan JR, Tompkins RG, Yarmush ML. Proline-mediated enhancement of hepatocyte function in a collagen gel sandwich culture configuration. FASEB J 1993;7:586-591.
20. Moghe PV, Berthiaume F, Ezzell RM, Toner M, Tompkins RG, Yarmush ML. Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function. Biomaterials 1996;17:373-385.
21. Schuppan D, Schmid M, Somasundaram R, Ackermann R, Ruehl M, Nakamura T, Riecken EO. Collagens in the liver extracellular matrix bind hepatocyte growth factor. Gastroenterology 1998;114:139-152.
22. Schonherr E, Hausser HJ. Extracellular matrix and cytokines: A functional unit. Dev Immunol 2000;7:89-101.