Unbiased cell-based screening in a neuronal cell model of batten disease highlights an interaction between Ca2+ homeostasis, autophagy, and CLN3 protein function

Journal of Biological Chemistry

Volumn 290, Issue 23, 2015, Pages 14361-14380

  Chandrachud, Uma ^a   Walker, Mathew W ^b   Simas, Alexandra M ^a   Heetveld, Sasja ^a   Petcherski, Anton ^a,c   Klein, Madeleine ^a   Oh, Hyejin ^a   Wolf, Pavlina ^a   Zhao, Wen Ning ^a   Norton, Stephanie ^a   Haggarty, Stephen J ^a   Lloyd Evans, Emyr ^b   Cotman, Susan L ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b CARDIFF UNIVERSITY   (United Kingdom)

^c GOETHE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CATALYST ACTIVITY; CELL MEMBRANES; CELLS; CYTOLOGY; DIAGNOSIS; NEURODEGENERATIVE DISEASES; NEURONS; PROTEINS;

CEROID LIPOFUSCINOSIS; ENDOPLASMIC RETICULUM; HUMAN-INDUCED PLURIPOTENT STEM CELLS; INTRACELLULAR CA; MICROTUBULE-ASSOCIATED PROTEIN 1-LIGHT CHAIN 3; NEURAL PROGENITOR CELLS; PROTEIN FUNCTIONS; SCREENING ASSAYS;

STEM CELLS;

ARVANIL; BAFILOMYCIN B1; BATTENIN; CALCIMYCIN; CALCIUM CHANNEL BLOCKING AGENT; CALCIUM ION; CHELERYTHRINE; ETHYLENE GLYCOL 1,2 BIS(2 AMINOPHENYL) ETHER N,N,N',N' TETRAACETIC ACID; GREEN FLUORESCENT PROTEIN; IKARUGAMYCIN; MEMBRANE PROTEIN; MICROTUBULE ASSOCIATED PROTEIN; MICROTUBULE ASSOCIATED PROTEIN 1 LIGHT CHAIN 3; N (3 PROPYLCARBAMOYLOXIRANE 2 CARBONYL)ISOLEUCYLPROLINE; N [N (3 CARBOXYOXIRANE 2 CARBONYL)LEUCYL]AGMATINE; NIFEDIPINE; PEPSTATIN; PROTEIN CLN3; RAPAMYCIN; SARCOPLASMIC RETICULUM CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE; THAPSIGARGIN; TUNICAMYCIN; UNCLASSIFIED DRUG; CALCIUM; CHAPERONE; CLN3 PROTEIN, HUMAN; CLN3 PROTEIN, MOUSE;

ARTICLE; AUTOPHAGOSOME; AUTOPHAGY; BIOACCUMULATION; CALCIUM CELL LEVEL; CALCIUM HOMEOSTASIS; CALCIUM METABOLISM; CELL ASSAY; CELL SCREENING; CELLULAR DISTRIBUTION; CHELATION; CLN3 GENE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISEASE MODEL; DRUG SCREENING; DRUG SENSITIVITY; DRUG TARGETING; ENDOPLASMIC RETICULUM; GENE; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE IDENTIFICATION; GENE INTERACTION; GENE LOCATION; GENE MUTATION; GENETIC SCREENING; GENOTYPE PHENOTYPE CORRELATION; HOMOZYGOSITY; HUMAN; HUMAN CELL; LC3 GENE; LYSOSOME; MITOCHONDRION; MODIFIER GENE; MOLECULAR INTERACTION; NERVE CELL; NERVE CELL CULTURE; NEURAL STEM CELL; NEURONAL CEROID LIPOFUSCINOSIS; PATHOPHYSIOLOGY; PILOT STUDY; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; REPRODUCIBILITY; SCREENING TEST; TRANSGENE; UPREGULATION; ANIMAL; CELL CULTURE; CELL LINE; DRUG EFFECTS; GENETICS; METABOLISM; MOUSE; MUTATION; NEURONAL CEROID-LIPOFUSCINOSES; PATHOLOGY; SIGNAL TRANSDUCTION;

ANIMALS; AUTOPHAGY; CALCIUM; CELL LINE; CELLS, CULTURED; DRUG EVALUATION, PRECLINICAL; HUMANS; MEMBRANE GLYCOPROTEINS; MICE; MOLECULAR CHAPERONES; MUTATION; NEURAL STEM CELLS; NEURONAL CEROID-LIPOFUSCINOSES; SIGNAL TRANSDUCTION;

EID: 84930667740     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.621706     Document Type: Article

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