Monitoring autophagic flux by an improved tandem fluorescent-tagged LC3 (mTagRFP-mWasabi-LC3) reveals that high-dose rapamycin impairs autophagic flux in cancer cells

Autophagy

Volumn 8, Issue 8, 2012, Pages 1215-1226

  Zhou, Cuihong ^a,b   Zhong, Wu ^c   Zhou, Jun ^b   Sheng, Fugeng ^d   Fang, Ziyuan ^b   Wei, Yue ^b   Chen, Yingyu ^e   Deng, Xiaoyan ^a   Xia, Bin ^b   Lin, Jian ^b  

^a BEIHANG UNIVERSITY   (China)

^b PEKING UNIVERSITY   (China)

^c BEIJING INSTITUTE OF PHARMACOLOGY AND TOXICOLOGY   (China)

^d BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^e PEKING UNIVERSITY   (China)

Author keywords

Autolysosome; Autophagic flux; Autophagosome; Autophagy; Cisplatin; LC3; Lysosome; mRFP EGFP LC3; mTagRFP m Wasabi LC3; Rapamycin; Staurosporine; Tandem fluorescent tagged LC3; Z18

Indexed keywords

CISPLATIN; GREEN FLUORESCENT PROTEIN; LIGHT CHAIN 3 I PROTEIN; PEPTIDES AND PROTEINS; RAPAMYCIN; STAUROSPORINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; AUTOLYSOSOME; AUTOPHAGOSOME; AUTOPHAGY; DOSE RESPONSE; EMBRYO; HELA CELL; LIGHT CHAIN; LYSOSOME; MOUSE; NONHUMAN; PH; PROTEIN LOCALIZATION; WASABI;

EUTREMA WASABI;

EID: 84862273857     PISSN: 15548627     EISSN: 15548635     Source Type: Journal    
DOI: 10.4161/auto.20284     Document Type: Article

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