High-throughput screens in mammalian cells using the CRISPR-Cas9 system

FEBS Journal

Volumn 282, Issue 11, 2015, Pages 2089-2096

  Peng, Jingyu ^a   Zhou, Yuexin ^a   Zhu, Shiyou ^a   Wei, Wensheng ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

CRISPR Cas9 system; high throughput; knockout; screening; sgRNA

Indexed keywords

LENTIVIRUS VECTOR; LONG UNTRANSLATED RNA; MICRORNA; RNA; SHORT HAIRPIN RNA; SINGLE GUIDE RNA; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; ENDONUCLEASE;

CELL CLONE; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; DOUBLE STRANDED DNA BREAK; ENHANCER REGION; FUNCTIONAL GENOMICS; GENE FUNCTION; GENE INACTIVATION; GENE LIBRARY; GENETIC TRANSCRIPTION; HIGH THROUGHPUT SCREENING; HIGH THROUGHPUT SEQUENCING; HUMAN; INDEL MUTATION; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REVIEW; RNA INTERFERENCE; ANIMAL; BIOASSAY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENE SILENCING; GENETIC ENGINEERING; GENETICS;

MAMMALIA;

ANIMALS; BACTERIAL PROTEINS; BIOLOGICAL ASSAY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; ENDONUCLEASES; GENE KNOCKDOWN TECHNIQUES; GENE LIBRARY; GENETIC ENGINEERING; HUMANS; RNA INTERFERENCE;

EID: 84930628716     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.13251     Document Type: Review

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