Absolute and multiplex quantification of antibodies in serum using PSAQ™ standards and LC-MS/MS

Bioanalysis

Volumn 7, Issue 10, 2015, Pages 1237-1251

  Lebert, Dorothee ^a   Picard, Guillaume ^a   Beau Larvor, Charlotte ^b   Troncy, Lysiane ^b   Lacheny, Christine ^b   Maynadier, Bernadette ^c   Low, Walter ^d   Mouz, Nicolas ^a   Brun, Virginie ^e,f,g   Klinguer Hamour, Christine ^b   Jaquinod, Michel ^e,f,g   Beck, Alain ^b  

^a Promise Advanced Proteomics   (France)

^b INSTITUT DE RECHERCHE PIERRE FABRE   (France)

^c Centre d'Immunologie Pierre Fabre   (France)

^d PX Therapeutics   (France)

^e UNIV GRENOBLE ALPES   (France)

^f CEA GRENOBLE   (France)

^g INSERM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; IMMUNOGLOBULIN G4; LYSINE; MONOCLONAL ANTIBODY; PROLINE; IMMUNOGLOBULIN G;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DRUG BLOOD LEVEL; DRUG CLEARANCE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MEASUREMENT ACCURACY; NONHUMAN; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; RAT; SINGLE DRUG DOSE; STANDARD; TANDEM MASS SPECTROMETRY; TITRIMETRY; ANIMAL; BLOOD; CHEMISTRY; EVALUATION STUDY; MOLECULAR GENETICS; PROCEDURES;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, LIQUID; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN G; MOLECULAR SEQUENCE DATA; RATS; TANDEM MASS SPECTROMETRY;

EID: 84930581841     PISSN: 17576180     EISSN: 17576199     Source Type: Journal    
DOI: 10.4155/bio.15.56     Document Type: Article

References (32)

1. Reichert JM, Beck A, Lugovskoy AA et al. 9th Annual European Antibody Congress, 11-13 November 2013, Geneva, Switzerland. MAbs 6(2), 309-326 (2014).
2. Maloney DG, Grillo-López AJ, White CA et al. Idec-c2b8 (rituximab) anti-cd20 monoclonal antibody therapy in patients with relapsed low-grade non-Hodgkin's lymphoma. Blood 90, 2188-2195 (1997).
3. Beck A, Wagner-Rousset E, Wurch T et al. Therapeutic antibodies and related products: choosing the right structure for success. Med. Sci. (Paris) 25, 1024-1032 (2009).
4. Beck A, Wurch T, Bailly C et al. Strategies and challenges for the next generation of therapeutic antibodies. Nat. Rev. Immunol. 10, 345-352 (2010).
5. Pan S, Aebersold R, Chen R et al. Mass spectrometry based targeted protein quantification: methods and applications. J. Proteome Res. 8, 787-797 (2009).
6. Gillette M. A, Carr SA. Quantitative analysis of peptides and proteins in biomedicine by targeted mass spectrometry. Nat. Methods 10, 28-34 (2013).
7. Hagman C, Ricke D, Ewert S et al. Absolute quantification of monoclonal antibodies in biofluids by liquid chromatography-tandem mass spectrometry. Anal. Chem. 80, 1290-1296 (2008).
8. Heudi O, Barteau S, Zimmer D et al. Towards absolute quantification of therapeutic monoclonal antibody in serum by LC-MS/MS using isotope-labeled antibody standard and protein cleavage isotope dilution mass spectrometry. Anal. Chem. 80, 4200-4207 (2008).
9. Furlong MT, Titsch C, Xu W et al. An exploratory universal lc-ms/ms assay for bioanalysis of hinge regionstabilized human IgG4 mabs in clinical studies. Bioanalysis 6, 1747-1758 (2014).
10. Jiang H, Zeng J, Titsch C et al. Fully validated LC-MS/MS assay for the simultaneous quantitation of coadministered therapeutic antibodies in cynomolgus monkey serum. Anal. Chem. 85, 9859-9867 (2013).
11. Liu G, Ji QC, Dodge R et al. Liquid chromatography coupled with tandem mass spectrometry for the bioanalysis of proteins in drug development: practical considerations in assay development and validation. J. Chromatogr. A 1284, 155-162 (2013).
12. Furlong MT, Zhao S, Mylott W et al. Dual universal peptide approach to bioanalysis of human monoclonal antibody protein drug candidates in animal studies. Bioanalysis 5, 1363-1376 (2013).
13. Brun V, Dupuis A, Adrait A et al. Isotope-labeled protein standards: toward absolute quantitative proteomics. Mol. Cell Proteomics 6, 2139-2149 (2007).
14. Picard G, Lebert D, Louwagie M et al. PSAQ™ standards for accurate MS-based quantification of proteins: from the concept to biomedical applications. J. Mass Spectrom. 47, 1353-1363 (2012).
15. Lebert D, Dupuis A, Garin J et al. Production and use of stable isotope-labeled proteins for absolute quantitative proteomics. Methods Mol. Biol. 753, 93-115 (2011).
16. Atmanene C, Wagner-Rousset E, Malissard M et al. Extending mass spectrometry contribution to therapeutic monoclonal antibody lead optimization: characterization of immune complexes using noncovalent ESI-MS. Anal. Chem. 81, 6364-6373 (2009).
17. Goetsch L, Haeuw J, Beau-Larvor C et al. A novel role for junctional adhesion molecule-a in tumor proliferation: modulation by an anti-JAM-A monoclonal antibody. Int. J. Cancer 132, 1463-1474 (2013).
18. Ayoub D, Diertaccini D, Diemer H et al. Characterization of the N-terminal heterogeneities of monoclonal antibodies by a new approach using TMPP in-gel derivatization and LC-MS/MS. Anal Chem. doi:10.1021/ac504427k (2015) (Epub ahead of print).
19. Boeuf A, Debaene F, Ayoub D et al. Mass spectrometry based strategies for therapeutic antibodies extensive characterization and optimization (optimabs). In: Structural Biology in Drug Discovery: Methods, Techniques, and Practices. Renaud JP (Ed.). John Wiley & Sons (2015) (In press).
20. MacLean B, Tomazela DM, Shulman N et al. Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics 26, 966-968 (2010).
21. Ezan E, Bitsch F. Critical comparison of MS and immunoassays for the bioanalysis of therapeutic antibodies. Bioanalysis 1, 1375-1388 (2009).
22. Chappell DL, Lassman ME, McAvoy T et al. Quantitation of human peptides and proteins via MS: review of analytically validated assays. Bioanalysis 6, 1843-1857 (2014).
23. Zhang Q, Spellman DS, Song Y et al. Generic automated method for liquid chromatography-multiple reaction monitoring mass spectrometry based monoclonal antibody quantitation for preclinical pharmacokinetic studies. Anal. Chem. 86, 8776-8784 (2014).
24. Xu K, Liu L, Maia M et al. A multiplexed hybrid LC-MS/MS pharmacokinetic assay to measure two co-administered monoclonal antibodies in a clinical study. Bioanalysis 6, 1781-1794 (2014).
25. Li H, Ortiz R, Tran LTB et al. Simultaneous analysis of multiple monoclonal antibody biotherapeutics by LC-MS/MS method in rat plasma following cassettedosing. AAPS J. 15, 337-346 (2013).
26. Li H, Ortiz R, Tran L et al. General LC-MS/MS method approach to quantify therapeutic monoclonal antibodies using a common whole antibody internal standard with application to preclinical studies. Anal. Chem. 84, 1267-1273 (2012).
27. Lössner C, Warnken U, Pscherer A et al. Preventing arginine-to-proline conversion in a cell-line-independent manner during cell cultivation under stable isotope labeling by amino acids in cell culture (silac) conditions. Anal. Biochem. 412, 123-125 (2011).
28. Louwagie M, Kieffer-Jaquinod S, Dupierris V et al. Introducing AAA-MS, a rapid and sensitive method for amino acid analysis using isotope dilution and high-resolution mass spectrometry. J. Proteome Res. 11, 3929-3936 (2012).
29. Brun V, Masselon C, Garin J et al. Isotope dilution strategies for absolute quantitative proteomics. J. Proteomics 72, 740-749 (2009).
30. Jaquinod M, Trauchessec M, Huillet C et al. Mass spectrometry-based absolute protein quantification: PSAQ™ strategy makes use of "noncanonical" proteotypic peptides. Proteomics 12, 1217-1221 (2012).
31. Beck A, Wagner-Rousset E, Ayoub D et al. Characterization of therapeutic antibodies and related products. Anal. Chem. 85, 715-736 (2013).
32. Raju TS, Strohl WR. Potential therapeutic roles for antibody mixtures. Expert Opin. Biol. Ther. 13, 1347-1352 (2013).