Protective effect of spironolactone on endothelial-to-mesenchymal transition in HUVECS via notch pathway

Cellular Physiology and Biochemistry

Volumn 36, Issue 1, 2015, Pages 191-200

  Chen, Xiao ^a,b   Cai, Jiejie ^a   Zhou, Xi ^a   Chen, Lingzhi ^c   Gong, Yongsheng ^d   Gao, Zhan ^a   Zhang, Huaiqin ^a   Huang, Weijian ^a   Zhou, Hao ^a  

^a THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIVERSITY   (China)

^b NINGBO FIRST HOSPITAL   (China)

^c WENZHOU CENTRAL HOSPITAL   (China)

^d WENZHOU MEDICAL UNIVERSITY   (China)

Author keywords

EndMT; Fibrosis; HUVEC; Notch pathway; Spironolactone; TGF

Indexed keywords

CD31 ANTIGEN; NOTCH RECEPTOR; NOTCH1 RECEPTOR; SPIRONOLACTONE; TRANSFORMING GROWTH FACTOR BETA; VIMENTIN; VON WILLEBRAND FACTOR;

ARTICLE; CELL ADHESION; CELL ASSAY; CELL INVASION; CELL MIGRATION; CELL POLARITY; CELL STRUCTURE; CELL TRANSDIFFERENTIATION; CHEMOTAXIS; COMPARATIVE STUDY; CONTROLLED STUDY; DOWN REGULATION; EPITHELIAL MESENCHYMAL TRANSITION; FIBROSIS; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; UMBILICAL VEIN ENDOTHELIAL CELL; UPREGULATION; WESTERN BLOTTING; DRUG EFFECTS; GENE EXPRESSION; METABOLISM;

ANTIGENS, CD31; EPITHELIAL-MESENCHYMAL TRANSITION; GENE EXPRESSION; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; RECEPTORS, NOTCH; SIGNAL TRANSDUCTION; SPIRONOLACTONE; TRANSFORMING GROWTH FACTOR BETA; VIMENTIN;

EID: 84929877600     PISSN: 10158987     EISSN: 14219778     Source Type: Journal    
DOI: 10.1159/000374063     Document Type: Article

References (27)

1. Piera-Velazquez S, Li Z, Jimenez SA: Role of endothelial-mesenchymal transition (EndoMT) in the pathogenesis of fibrotic disorders. Am J Pathol 2011;179:1074-1080.
2. Yang T, Chen M, Sun T: Simvastatin attenuates TGF-1-induced epithelial-mesenchymal transition in human alveolar epithelial cells. Cell Physiol Biochem 2013;31:863-874.
3. Rieder F, Kessler SP, West GA, Bhilocha S, de la Motte C, Sadler TM, Gopalan B, Stylianou E, Fiocchi C: Inflammation-induced endothelial-to-mesenchymal transition: a novel mechanism of intestinal fibrosis. Am J Pathol 2011;179:2660-2673.
4. Lin F, Wang N, Zhang TC: The Role of Endothelial-Mesenchymal Transition in Development and Pathological Process. Cell Mol Biol 2012;64:717-723.
5. Zeisberg EM, Potenta SE, Sugimoto H, Zeisberg M, Kalluri R: Fibroblasts inkidney fibrosis emerge via endothelial-to-mesenchymaltransition. J Am SocNephrol JASN 2008;19:2282-2287.
6. Samarakoon R, Overstreet JM, Higgins P J: TGF- signaling in tissue fibrosis: Redox controls, target genes and therapeutic opportunities. Cell Signal 2013;25:264-268.
7. Samy Lamouille, Jian Xu, Rik Derynck: Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol 2014;15:178-196.
8. Chen YX, Zheng SP, Qi D, Zheng S, Guo J, Zhang SL, Weng Z-H: Inhibition of Notch Signaling by a -Secretase Inhibitor Attenuates Hepatic Fibrosis in Rats. Plos One 2012;7:e46512.
9. Wang L, Wang YC, Hu XB, Zhang BF, Dou GR, He F, Gao F, Feng F, Liang YM, Dou KF, Han H: Notch-RBP-J Signaling Regulates the Mobilization and Function of Endothelial Progenitor Cells by Dynamic Modulation of CXCR4 Expression in Mice. Plos One 2009;4:e7572.
10. P. Magni, M. Motta: Aldosterone receptor antagonists: Biology and novel therapeutical applications. J Endocrinol Invest 2003;26:788-798.
11. Nemir M, Metrich M, Plaisance I, Lepore M, Cruchet S, Berthonneche C, Sarre A, Radtke F, Pedrazzini T: The Notch pathway controls fibrotic and regenerativerepair in the adult heart. Eur. Heart J 2012;35:2174-2185.
12. Zeisberg EM, Tarnavski O, Zeisberg M, Dorfman AL, McMullen JR, Gustafsson E, Chandraker A, Yuan X, Pu WT, Roberts AB, Neilson EG, Sayegh MH, Izumo S, Kalluri R: Endothelial to mesenchymal transition contributes to cardiac fibrosis. Nat med 2007;8:952-961.
13. Zhang L, Hao JB, Ren L-S, Ding JL, Hao LR: The aldosterone receptor antagonist spironolactone prevents peritoneal inflammation and fibrosis. Lab Invest 2014;94:839-850.
14. Chen H, Sun F, Zhong X, Shao Y, Yoshimura A, Liu Y: Eplerenone-mediated aldosterone blockade prevents renal fibrosis by reducing renal inflammation, interstitial cell proliferation and oxidative stress. Kidney Blood Press Res 2013;37:557-566.
15. Luo JH, Gao XR, Peng LY, Sun HW, Dai G: Effects of hydrochlorothiazide on cardiac remodeling in a rat model of myocardial infarction-induced congestive heart failure. Eur J Pharmacol 2011;667:314-321.
16. Vardeny O, Cavallari LH, Claggett B, Desai AS, Anand I, Rossignol P, Zannad F, Pitt B, Solomon SD: Race Influences the Safety and Efficacy of Spironolactone in Severe Heart Failure. Circ Heart Fail 2013;6:970-976.
17. Katsuhito F, Ryozo N: Fibroblast-mediated pathways in cardiac hypertrophy. J Mol Cell Cardiol 2014;70:64-73.
18. Zhou H, Chen X, Chen LZ, Zhou X, Zheng GS, Zhang HQ, Huang WJ, Cai JJ: Anti-Fibrosis Effect of Scutellarin via Inhibition of Endothelial-Mesenchymal Transitionon Isoprenaline-Induced Myocardial Fibrosis in Rats. Molecules 2014;19:15611-15623.
19. Fan D, Abhijit T, Jiwon L, Zamaneh K: Cardiac fibroblasts, fibrosis and extracellular matrix remodeling in heart disease. Fibrogenesis& Tissue Repair 2012;5:15.
20. Zeisberg EM, Potenta S, Xie L, Zeisberg M, Kalluri R: Discovery of endothelial to mesenchymal transition as a source for carcinoma-associated fibroblasts. Cancer Res 2007;67:10123-10128.
21. Ruiz-Ortega M, Rodriguez-Vita J, Sanchez-Lopez E, Carvajal G, Egido J: TGF-beta signaling in vascular fibrosis. Cardiovasc Res 2007;74:196-206.
22. Barter MJ, Pybus L, Litherland GJ, Rowan AD, Clark IM, Edwards DR, Cawston TE, Young DA: HDAC-mediated control of ERK and PI3K-dependent TGF--induced extracellular matrix-regulating genes. Matrix Biol 2010;29:602-612.
23. Brilla CG, Matsubara LS, Weber KT: Antifibrotic effects of spironolactone in preventing myocardial fibrosis in systemic arterial hypertension. Am J Cardiol 1993;71:12A-16A.
24. Xiao Z, Zhang J, Peng X, Dong Y, Jia L, Li H, Du J: The Notch -secretase inhibitor ameliorates kidney fibrosis via inhibition of TGF-/Smad2/3 signaling pathway activation. Int J Biochem Cell Biol 2014;55:65-71.
25. Giani M, Mastrangelo A, Villa R, Turolo S, Marra G, Tirelli AS, Hopfer H, Edefonti A: Alport syndrome: the effects of spironolactone on proteinuria and urinary TGF-1. Pediatr Nephrol 2013;28:1837-1842.
26. Li C, Dong F, Jia Y, Du HY, Dong N, Xu YJ, Wang S, Wu HP, Liu ZG, Li W: Notch signal regulates corneal endothelial-to-mesenchymal transition. Am J Pathol 2013;183:786-795.
27. Edgley AJ, Krum H, Kelly DJ: Targeting Fibrosis for the Treatment of Heart Failure: A Role for Transforming Growth Factor-?. Cardiovasc Ther 2012;30:e30-40.