Biochemical characterization of recombinant enterovirus 71 3c protease with fluorogenic model peptide substrates and development of a biochemical assay

Antimicrobial Agents and Chemotherapy

Volumn 59, Issue 4, 2015, Pages 1827-1836

  Shang, Luqing ^a,b   Zhang, Shumei ^a,b   Yang, Xi ^a,b   Sun, Jixue ^a   Li, Linfeng ^a,b   Cui, Zhengjie ^a,b   He, Qiuhong ^c   Guo, Yu ^a   Sun, Yuna ^d   Yin, Zheng ^a,b  

^a NANKAI UNIVERSITY   (China)

^b SynBio Research Platform   (China)

^c Tianjin International Joint Academy of Biotechnology and Medicine   (China)

^d INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ENTEROVIRUS 71 3C PROTEASE; FLUOROGENIC PEPTIDE; PEPTIDE DERIVATIVE; PROTEINASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; VIRUS PROTEIN; 3C PROTEASES; ANTIVIRUS AGENT; CYSTEINE PROTEINASE; FLUORESCENT DYE; PEPTIDE; PROTEINASE INHIBITOR;

AMINO ACID SUBSTITUTION; ARTICLE; BIOCHEMICAL ANALYSIS; CELL PROLIFERATION; CONTROLLED STUDY; CYTOTOXIC CONCENTRATION; CYTOTOXICITY; EFFECTIVE CONCENTRATION; ENTEROVIRUS 71; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SPECIFICITY; HUMAN; HUMAN CELL; IN VITRO STUDY; MICHAELIS MENTEN KINETICS; MOLECULAR DOCKING; MOLECULAR DYNAMICS; NONHUMAN; PEPTIDE SYNTHESIS; PH; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RHABDOMYOSARCOMA; SUBSTRATE CONCENTRATION; TURNOVER NUMBER; VIRUS REPLICATION; AMINO ACID SEQUENCE; ANTAGONISTS AND INHIBITORS; BIOASSAY; BIOSYNTHESIS; CELL LINE; CELL SURVIVAL; DRUG EFFECTS; ENTEROVIRUS; ENZYMOLOGY; FOOT AND MOUTH DISEASE VIRUS; KINETICS; MOLECULAR GENETICS; SYNTHESIS;

AMINO ACID SEQUENCE; ANTIVIRAL AGENTS; BIOLOGICAL ASSAY; CELL LINE; CELL SURVIVAL; CYSTEINE ENDOPEPTIDASES; ENTEROVIRUS; FLUORESCENT DYES; FOOT-AND-MOUTH DISEASE VIRUS; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEASE INHIBITORS; SUBSTRATE SPECIFICITY; VIRAL PROTEINS;

EID: 84928910517     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.04698-14     Document Type: Article

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