Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody

Malaria Journal

Volumn 14, Issue 1, 2015, Pages

  Kapelski, Stephanie ^a,b   Boes, Alexander ^a   Spiegel, Holger ^a   De Almeida, Melanie ^a,b   Klockenbring, Torsten ^a   Reimann, Andreas ^a   Fischer, Rainer ^a,b   Barth, Stefan ^a,b   Fendel, Rolf ^a,b  

^a FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Antibody characterization; Hybridoma antibody cloning; Nicotiana benthamiana; Plant based production; Plasmodium falciparum; Transient antibody expression; Vacuum infiltration

Indexed keywords

COMPLEMENTARY DNA; EPIDERMAL GROWTH FACTOR; GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORED PROTEIN; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 4; RECOMBINANT ANTIBODY; UNCLASSIFIED DRUG; IMMUNOGLOBULIN VARIABLE REGION; MEROZOITE SURFACE PROTEIN 4, PLASMODIUM; PARASITE ANTIGEN; PROTOZOAL PROTEIN; PROTOZOON ANTIBODY; RECOMBINANT PROTEIN;

ANTIBODY STRUCTURE; ANTIBODY VARIABLE DOMAIN; ARTICLE; DNA SYNTHESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; GENE AMPLIFICATION; HYBRIDOMA CELL LINE; IMMUNOFLUORESCENCE TEST; MOLECULAR CLONING; NICOTIANA BENTHAMIANA; NONHUMAN; PROTEIN DOMAIN; PROTEIN EXPRESSION; RNA ISOLATION; SCHIZONT; SEQUENCE ANALYSIS; SURFACE PLASMON RESONANCE; WESTERN BLOTTING; ANIMAL; BAGG ALBINO MOUSE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; TOBACCO; TRANSGENIC ORGANISM;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, PROTOZOAN; ANTIGENS, PROTOZOAN; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MICE, INBRED BALB C; MICROSCOPY, FLUORESCENCE; ORGANISMS, GENETICALLY MODIFIED; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SURFACE PLASMON RESONANCE; TOBACCO;

EID: 84928727494     PISSN: None     EISSN: 14752875     Source Type: Journal    
DOI: 10.1186/s12936-015-0577-7     Document Type: Article

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