Synchronous culture of Plasmodium falciparum at high parasitemia levels

Nature Protocols

Volumn 4, Issue 12, 2009, Pages 1899-1915

  Radfar, Azar ^a,b   Méndez, Darío ^a   Moneriz, Carlos ^a   Linares, María ^a   Marín García, Patricia ^a   Puyet, Antonio ^a   Diez, Amalia ^a   Bautista, José M ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LIQUID NITROGEN; SORBITOL; CULTURE MEDIUM; PROTOZOAL PROTEIN;

ACCURACY; ARTICLE; BLOOD PARASITE; BLOOD SMEAR; CELL INVASION; CRYOPRESERVATION; ERYTHROCYTE LIFESPAN; HEMATOCRIT; MICROBIAL GROWTH; NONHUMAN; PARASITE CULTIVATION; PARASITEMIA; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; TROPHOZOITE; CULTURE MEDIUM; CULTURE TECHNIQUE; ERYTHROCYTE; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PARASITOLOGY;

PLASMODIUM FALCIPARUM;

CELL CULTURE TECHNIQUES; CULTURE MEDIA; ERYTHROCYTES; HUMANS; PLASMODIUM FALCIPARUM; PROTOZOAN PROTEINS;

EID: 73849120110     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.198     Document Type: Article

References (47)

1. Schuster, F.L. Cultivation of Plasmodium spp. Clin. Microbiol. Rev. 15, 355-364 (2002).
2. Ferrer, J. et al. Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures. Malar. J. 7, 203 (2008).
3. Orjih, A.U. Requirements for maximal enrichment of viable intraerythrocytic Plasmodium falciparum rings by saponin hemolysis. Exp. Biol. Med. (Maywood) 233, 1359-1367 (2008).
4. Trager, W. & Jensen, J.B. Human malaria parasites in continuous culture. Science 193, 673-675 (1976).
5. Trager, W. & Jensen, J.B. Continuous culture of Plasmodium falciparum: its impact on malaria research. Int. J. Parasitol. 27, 989-1006 (1997).
6. Divo, A.A., Vande Waa, J.A., Campbell, J.R. & Jensen, J.B. Isolation and cultivation of Plasmodium falciparum using adult bovine serum. J. Parasitol. 71, 504-509 (1985).
7. Trager, W. & Jensen, J.B. Cultivation of erythrocytic stages. Bull. World Health Organ. 55, 363-365 (1977).
8. Lingnau, A., Margos, G., Maier, W.A. & Seitz, H.M. Serum-free cultivation of several Plasmodium falciparum strains. Parasitol. Res. 80, 84-86 (1994).
9. Flores, M.V., Berger-Eiszele, S.M. & Stewart, T.S. Long-term cultivation of Plasmodium falciparum in media with commercial non-serum supplements. Parasitol. Res. 83, 734-736 (1997).
10. Chin, W., Moss, D. & Collins, W.E. The continuous cultivation of Plasmodium fragile by the method of Trager-Jensen. Am. J. Trop. Med. Hyg. 28, 591-592 (1979).
11. Binh, V.Q., Luty, A.J. & Kremsner, P.G. Differential effects of human serum and cells on the growth of Plasmodium falciparum adapted to serum-free in vitro culture conditions. Am. J. Trop. Med. Hyg. 57, 594-600 (1997).
12. Zolg, J.W., MacLeod, A.J., Dickson, I.H. & Scaife, J.G. Plasmodium falciparum: modifications of the in vitro culture conditions improving parasitic yields. J. Parasitol. 68, 1072-1080 (1982).
13. Singh, K., Agarwal, A., Khan, S.I., Walker, L.A. & Tekwani, B.L. Growth, drug susceptibility, and gene expression profiling of Plasmodium falciparum cultured in medium supplemented with human serum or lipid-rich bovine serum albumin [corrected]. J. Biomol. Screen. 12, 1109-1114 (2007).
14. Trager, W. & Jensen, J.B. Cultivation of malarial parasites. Nature 273, 621-622 (1978).
15. Jensen, J.B. In vitro culture of Plasmodium falciparum parasites. in Malaria Methods and Protocols (ed. Doolan, D.L.) 477-488 (Humana Press, Totowa, New Jersey, 2002).
16. Raventos-Suarez, C. Plasmodium falciparum: invasion and development in highly parasitized cultures. In Vitro Cell Dev. Biol. 21, 161-164 (1985).
17. Vernot, J.P. & Wasserman, M. Plasmodium falciparum increased and multiple invasion during short periods of time. J. Protozool. 37, 47-49 (1990).
18. Radfar, A., Diez, A. & Bautista, J.M. Chloroquine mediates specific proteome oxidative damage across the erythrocytic cycle of resistant Plasmodium falciparum. Free Radic. Biol. Med. 44, 2034-2042 (2008).
19. Gardner, M.J. et al. Genome sequence of the human malaria parasite Plasmodium falciparum. Nature 419, 498-511 (2002).
20. Ley, T.J. et al. DNA sequencing of a cytogenetically normal acute myeloid leukaemia genome. Nature 456, 66-72 (2008).
21. Lasonder, E. et al. Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry. Nature 419, 537-542 (2002).
22. Florens, L. et al. A proteomic view of the Plasmodium falciparum life cycle. Nature 419, 520-526 (2002).
23. Sims, P.F. & Hyde, J.E. Proteomics of the human malaria parasite Plasmodium falciparum. Expert Rev. Proteomics 3, 87-95 (2006).
24. Ribacke, U. Growing Plasmodium falciparum cultures at high parasitemia. in Methods in Malaria Research (eds. Moll, K., Perlmann, H., Scherf, A. & Wahlgren, M.) 8 (American Type Culture Collection, Manassas, Virginia, 2008).
25. Li, T., Glushakova, S. & Zimmerberg, J. A new method for culturing Plasmodium falciparum shows replication at the highest erythrocyte densities. J. Infect. Dis. 187, 159-162 (2003).
26. Rivadeneira, E.M., Wasserman, M. & Espinal, C.T. Separation and concentration of schizonts of Plasmodium falciparum by Percoll gradients. J. Protozool. 30, 367-370 (1983).
27. Trager, W. & Lanners, H.N. Initial extracellular development in vitro of merozoites of Plasmodium falciparum. J. Protozool. 31, 562-567 (1984).
28. Fernandez, V. Enrichment of late-stage infected erythrocytes in 60% Percoll. in Methods in Malaria Research (eds. Moll, K., Perlmann, H., Scherf, A. & Wahlgren, M.) 25 (American Type Culture Collection, Manassas, Virginia, 2008).
29. Lelievre, J., Berry, A. & Benoit-Vical, F. An alternative method for Plasmodium culture synchronization. Exp. Parasitol. 109, 195-197 (2005).
30. Fernandez, V. Separation of Plasmodium falciparum mature stages in Percoll/sorbitol gradients. in Methods in Malaria Research (eds. Moll, K., Perlmann, H., Scherf, A. & Wahlgren, M.) 26-27 (American Type Culture Collection, Manassas, Virginia, 2008).
31. Ferrer, J. et al. Individual-based model and simulation of Plasmodium falciparum infected erythrocyte in vitro cultures. J. Theor. Biol. 248, 448-459 (2007).
32. Jensen, J.B. Concentration from continuous culture of erythrocytes infected with trophozoites and schizonts of Plasmodium falciparum. Am. J. Trop. Med. Hyg. 27, 1274-1276 (1978).
33. Jensen, J.B. & Trager, W. Plasmodium falciparum in culture: establishment of additional strains. Am. J. Trop. Med. Hyg. 27, 743-746 (1978). (Pubitemid 8398075)
34. Ofulla, A.V. et al. Cultivation of Plasmodium falciparum parasites in a serum-free medium. Am. J. Trop. Med. Hyg. 49, 335-340 (1993).
35. Zolg, J.W., Macleod, A.J., Scaife, J.G. & Beaudoin, R.L. The accumulation of lactic acid and its influence on the growth of Plasmodium falciparum in synchronized cultures. In Vitro 20, 205-215 (1984).
36. Jensen, M.D., Conley, M. & Helstowski, L.D. Culture of Plasmodium falciparum: the role of pH, glucose, and lactate. J. Parasitol. 69, 1060-1067 (1983).
37. Lambros, C. & Vanderberg, J.P. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J. Parasitol. 65, 418-420 (1979).
38. Wahlgren, M., Berzins, K., Perlmann, P. & Bjorkman, A. Characterization of the humoral immune response in Plasmodium falciparum malaria. I. Estimation of antibodies to P. falciparum or human erythrocytes by means of microELISA. Clin. Exp. Immunol. 54, 127-134 (1983).
39. Kutner, S., Breuer, W.V., Ginsburg, H., Aley, S.B. & Cabantchik, Z.I. Characterization of permeation pathways in the plasma membrane of human erythrocytes infected with early stages of Plasmodium falciparum: association with parasite development. J. Cell. Physiol. 125, 521-527 (1985).
40. Krugliak, M. & Ginsburg, H. The evolution of the new permeability pathways in Plasmodium falciparum-infected erythrocytes-a kinetic analysis. Exp. Parasitol. 114, 253-258 (2006).
41. Krugliak, M., Zhang, J. & Ginsburg, H. Intraerythrocytic Plasmodium falciparum utilizes only a fraction of the amino acids derived from the digestion of host cell cytosol for the biosynthesis of its proteins. Mol. Biochem. Parasitol. 119, 249-256 (2002).
42. Moody, A. Rapid diagnostic tests for malaria parasites. Clin. Microbiol. Rev. 15, 66-78 (2002).
43. World Health Organization. Staining Blood Films with Giemsa Stain (WHO Press, Geneva, Switzerland, 1991).
44. Goodyer, I.D., Johnson, J., Eisenthal, R. & Hayes, D.J. Purification of mature-stage Plasmodium falciparum by gelatine flotation. Ann. Trop. Med. Parasitol. 88, 209-211 (1994).
45. Pasvol, G., Weatherall, D.J. & Wilson, R.J. Effects of foetal haemoglobin on susceptibility of red cells to Plasmodium falciparum. Nature 270, 171-173 (1977).
46. Proudfoot, O., Drew, N., Scholzen, A., Xiang, S. & Plebanski, M. Investigation of a novel approach to scoring Giemsa-stained malaria-infected thin blood films. Malar. J. 7, 62 (2008).
47. Reilly, H.B., Wang, H., Steuter, J.A., Marx, A.M. & Ferdig, M.T. Quantitative dissection of clone-specific growth rates in cultured malaria parasites. Int. J. Parasitol. 37, 1599-1607 (2007).