Two amino acid mutations in an anti-human CD3 single chain Fv antibody fragment that affect the yield on bacterial secretion but not the affinity

Protein Engineering

Volumn 10, Issue 4, 1997, Pages 445-453

  Kipriyanov, Sergey M ^a   Moldenhauer, Gerhard ^a   Martin, Andrew C R ^b   Kupriyanova, Olga A ^a   Little, Melvyn ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Affinity; Anti human CD3; Bacterial expression; Single chain Fv; Solubility

Indexed keywords

CD3 ANTIGEN; CYSTEINE; GLUTAMIC ACID; IMMUNOGLOBULIN FRAGMENT; MEMBRANE ANTIGEN; MONOCLONAL ANTIBODY CD3; OKT 3; SERINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTIBODY AFFINITY; ANTIBODY PRODUCTION; ANTIBODY STRUCTURE; ANTIGEN BINDING; ARTICLE; CONTROLLED STUDY; DRUG STABILITY; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE MUTATION; GENETIC ENGINEERING; IMMUNOBLOTTING; MOLECULAR MODEL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN FOLDING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MURINAE;

EID: 0030916353     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.4.445     Document Type: Article

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