A robust method for increasing Fc glycan high mannose level of recombinant antibodies

Biotechnology and Bioengineering

Volumn 112, Issue 6, 2015, Pages 1200-1209

  Huang, Chung Jr ^a   Lin, Henry ^a,b   Yang, Jerry Xiaoming ^a,c  

^a AMGEN INC   (United States)

^b Boehringer Ingelheim   (United States)

^c DZM Biotech Ltd   (United States)

Author keywords

Antibody fc glycans; Chinese hamster ovary (CHO) cells; High mannose level modulation; Recombinant antibody

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CARBON; CELL CULTURE; CELLS; CYTOLOGY; MODULATION; POLYSACCHARIDES; PROTEINS;

CHINESE HAMSTER OVARY CELLS; CRITICAL QUALITY; GLYCANS; N-ACETYLGLUCOSAMINE; PROCESS DEVELOPMENT; PROTEIN GLYCOSYLATION; RECOMBINANT ANTIBODY; THERAPEUTIC ANTIBODIES;

ANTIBODIES;

FC GLYCAN; FRUCTOSE; GALACTOSE; GLUCOSE; GLYCAN; GUANOSINE DIPHOSPHATE MANNOSE; HEXOSE; MALTOSE; MANNOSE; N ACETYLGLUCOSAMINE; RECOMBINANT ANTIBODY; UNCLASSIFIED DRUG; CARBON; CULTURE MEDIUM; IMMUNOGLOBULIN FC FRAGMENT; RECOMBINANT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY PRODUCTION; ANTIBODY TITER; ARTICLE; BIOREACTOR; BIOSYNTHESIS; CARBON SOURCE; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL VIABILITY; CHO CELL LINE; CONTROLLED STUDY; CULTURE MEDIUM; IMMUNOMODULATION; NONHUMAN; PROCESS DEVELOPMENT; PROTEIN ANALYSIS; PROTEIN GLYCOSYLATION; SCALE UP; ANIMAL; CHEMISTRY; CRICETULUS; GLYCOSYLATION; METABOLISM; PROTEIN PROCESSING;

CRICETULUS GRISEUS;

ANIMALS; CARBON; CHO CELLS; CRICETULUS; CULTURE MEDIA; GLUCOSE; GLYCOSYLATION; IMMUNOGLOBULIN FC FRAGMENTS; MANNOSE; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS;

EID: 84928429962     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25534     Document Type: Article

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